Trails of green alga hydrogen research — from Hans Gaffron to new frontiers

Melis, Anastasios; Happe, Thomas

doi:10.1007/1-4020-3324-9_60

Cited by 7 publications

(17 citation statements)

References 70 publications

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“…, 1989; Boichenko et al. , 2004; Melis & Happe, 2004), and the precise physiological function of hydrogenase and H 2 synthesis in algal cells is not absolutely resolved. However, H 2 production is likely to have significant impacts on redox poising, photoprotection, and fermentative energy production.…”

Section: Hydrogenase In Algaementioning

confidence: 99%

“…, 2007) and the development of molecular tools applicable to studies of this alga (Harris, 2001; Grossman, 2007; Purton, 2007), and because of its ability to grow on a fixed carbon source in the absence of photosynthesis, C. reinhardtii has become an attractive reference system for dissecting various biological processes including flagellar function and assembly, cell cycle control, photosynthesis, chloroplast biogenesis and acclimation to changing nutrient conditions. Moreover, this green alga can synthesize molecular hydrogen (H 2 ) under anoxic conditions, which suggests its potential use for biofuel production (Melis & Happe, 2001, 2004; Ghirardi et al. , 2007).…”

Section: Introductionmentioning

confidence: 99%

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Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii

et al. 2010

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SummaryMany microbes in the soil environment experience micro-oxic or anoxic conditions for much of the late afternoon and night, which inhibit or prevent respiratory metabolism. To sustain the production of energy and maintain vital cellular processes during the night, organisms have developed numerous pathways for fermentative metabolism. This review discusses fermentation pathways identified for the soil-dwelling model alga Chlamydomonas reinhardtii, its ability to produce molecular hydrogen under anoxic conditions through the activity of hydrogenases, and the molecular flexibility associated with fermentative metabolism that has only recently been revealed through the analysis of specific mutant strains.

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Section: Hydrogenase In Algaementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii

et al. 2010

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“…Hydrogenase activity is well documented in eukaryotic algae [15,[31][32][33], with the green alga C. reinhardtii being the primary model system for investigating algal H 2 photoproduction [7,34]. Hydrogen producing conditions in both the light and the dark have been described.…”

Section: Hydrogen Production In Green Algaementioning

confidence: 99%

“…Intriguingly, the [FeFe]-hydrogenase enzymes evolved at some point to effectively interact with the photosynthetic electron transport chain in many eukaryotic algae, and this has been explored extensively for the sustained photoproduction of H 2 in these organisms [7,34]. Intriguingly, the [FeFe]-hydrogenase enzymes evolved at some point to effectively interact with the photosynthetic electron transport chain in many eukaryotic algae, and this has been explored extensively for the sustained photoproduction of H 2 in these organisms [7,34].…”

Section: Core Anaerobic Metabolisms In Eukaryotesmentioning

confidence: 99%

7 Hydrogenase evolution and function in eukaryotic algae

D’Adamo¹,

Posewitz²

2015

Biohydrogen

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“…‐[Ni-Fe]-hydrogenase: membrane bound, mainly for uptake of hydrogen, found in aerobic, anaerobic and facultative anaerobic bacteria [6, 3]. …”

Section: Introductionmentioning

confidence: 99%

Structure prediction and molecular simulation of gases diffusion pathways in hydrogenase

Sundaram¹,

Tripathi²,

Gupta³

2010

Bioinformation

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Although hydrogen is considered to be one of the most promising future energy sources and the technical aspects involved in using it have advanced considerably, the future supply of hydrogen from renewable sources is still unsolved. The [Fe]- hydrogenase enzymes are highly efficient H2 catalysts found in ecologically and phylogenetically diverse microorganisms, including the photosynthetic green alga, Chlamydomonas reinhardtii. While these enzymes can occur in several forms, H2 catalysis takes place at a unique [FeS] prosthetic group or H-cluster, located at the active site. 3D structure of the protein hydA1 hydrogenase from Chlamydomonas reinhardtti was predicted using the MODELER 8v2 software. Conserved region was depicted from the NCBI CDD Search. Template selection was done on the basis NCBI BLAST results. For single template 1FEH was used and for multiple templates 1FEH and 1HFE were used. The result of the Homology modeling was verified by uploading the file to SAVS server. On the basis of the SAVS result 3D structure predicted using single template was chosen for performing molecular simulation. For performing molecular simulation three strategies were used. First the molecular simulation of the protein was performed in solvated box containing bulk water. Then 100 H2 molecules were randomly inserted in the solvated box and two simulations of 50 and 100 ps were performed. Similarly 100 O2 molecules were randomly placed in the solvated box and again 50 and 100 ps simulation were performed. Energy minimization was performed before each simulation was performed. Conformations were saved after each simulation. Analysis of the gas diffusion was done on the basis of RMSD, Radius of Gyration and no. of gas molecule/ps plot.

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Trails of green alga hydrogen research — from Hans Gaffron to new frontiers

Cited by 7 publications

References 70 publications

Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii

Multiple facets of anoxic metabolism and hydrogen production in the unicellular green alga Chlamydomonas reinhardtii

7 Hydrogenase evolution and function in eukaryotic algae

Structure prediction and molecular simulation of gases diffusion pathways in hydrogenase

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