Tragia L. Genus: Ethnopharmacological Use, Phytochemical Composition and Biological Activity

Duarte-Casar, Rodrigo; Romero-Benavides, Juan Carlos

doi:10.3390/plants10122717

Cited by 6 publications

(3 citation statements)

References 77 publications

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“…Although there chemical composition ofT. hispida , is not known it has been shown that related species such as T. involucrata is rich in flavonoids, particularly in several glycosides and derivatives of quercetin 15,21 . Flavonoids have shown to inhibit sPLA2 activity, and has shown to inhibit sPLA2 in synovial fluid but not from pancreatic fluid, showing that T.hispidia is most likely to have compounds that inhibit sPLA2 II group of enzymes 22 .…”

Section: Characterization Secondary Metabolites Of Butanol Fraction O...mentioning

confidence: 99%

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

Hemalika,

Chandrika,

Abeysekera

et al. 2024

Preprint

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Background: As secretory phospholipase A2 (sPLA2) was shown to be elevated in patients who progress to severe dengue, it would be important to evaluate the usefulness of therapeutics that inhibit sPLA2 enzymes to prevent progression to severe dengue. Methods: Aqueous and butanol extracts of Tragia hispida, Justicia adathoda and tubers of Cyperus rotundus were screened for the presence of potential sPLA2 inhibitors using a commercial assay measuring sPLA2 activity. Results: Both the aqueous (THA) and butanol extracts (THB) of Tragia hispida had sPLA2 inhibition levels comparable to the levels seen with the commercial sPLA2 inhibitor CAY10590. THB at concentrations of 0.1 µg/µL and 0.2 µg/µL, significantly reduced the sPLA2 activity (p<0.0001) in the sera of dengue patients and the inhibitory activity was significantly higher (p<0.0001) than of CAY10590. Thin layer chromatography of THB showed that it was likely to contain a mixture of flavonoid and phenolic compounds. HPLC displayed peaks at 3.207 min (λmax 222 nm, 272 nm) and 7.972 min (λmax 224 nm, 272 nm) which were most likely to represent phenolics and peaks at 11.883 min (λmax 276 nm, 366 nm) and 16.898 min ( λmax 254 nm, 370 nm) which were most likely to represent flavonoids. Conclusions: T. hispida aqueous and butanol soluble fraction had potent sPLA2 inhibitory activities, which should be further explored for their potential to be used for treatment of dengue.

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Section: Characterization Secondary Metabolites Of Butanol Fraction O...mentioning

confidence: 99%

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

Hemalika,

Chandrika,

Abeysekera

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Although there chemical composition of T. hispida, is not known it has been shown that related species such as T. involucrata is rich in flavonoids, particularly in several glycosides and derivatives of quercetin [15,21]. Flavonoids have shown to inhibit sPLA2 activity, and has shown to inhibit sPLA2 in synovial fluid but not from pancreatic fluid, showing that T.hispidia is most likely to have compounds that inhibit sPLA2 II group of enzymes [22].…”

Section: Characterization Secondary Metabolites Of Butanol Fraction O...mentioning

confidence: 99%

Characterization of secretory phospholipase A2 inhibitory activity inTragia hispidaas potential therapeutic agent for treatment of dengue

Hemalika

Chandrika

Abeysekera

et al. 2022

Preprint

View full text Add to dashboard Cite

BackgroundAs secretory phospholipase A2 (sPLA2) was shown to be elevated in patients who progress to severe dengue, it would be important to evaluate the usefulness of therapeutics that inhibit sPLA2 enzymes to prevent progression to severe dengue.ObjectivesTo explore the presence of sPLA2 inhibitors in plant extracts used in traditional medicine for treatment of fever in Sri Lanka.Study designAqueous and butanol extracts ofTragia hispida, Justicia adathodaand tubers ofCyperus rotunduswere screened for the presence of potential sPLA2 inhibitors using a commercial assay measuring sPLA2 activity.ResultsBoth the aqueous (THA) and butanol extracts (THB) ofTragia hispidahad sPLA2 inhibition levels comparable to the levels seen with the commercial sPLA2 inhibitor CAY10590. THB at concentrations of 0.1 µg/µL and 0.2 µg/µL, significantly reduced the sPLA2 activity (p<0.0001) in the sera of dengue patients and the inhibitory activity was significantly higher (p<0.0001) than of CAY10590. Thin layer chromatography of THB showed that it was likely to contain a mixture of flavonoid and phenolic compounds. HPLC displayed peaks at 3.207 min (λmax 222 nm, 272 nm) and 7.972 min (λmax 224 nm, 272 nm) were most likely to represent phenolics and that the peaks at 11.883 min (λmax 276 nm, 366 nm) and 16.898 min (λmax 254 nm, 370 nm) most likely to represent flavonoids.ConclusionsT. hispidaaqueous and butanol soluble fraction had potent sPLA2 inhibitory activities, which should be further explored for their potential to be used for treatment of dengue.

show abstract

Section: Characterization Secondary Metabolites Of Butanol Fraction O...mentioning

confidence: 99%

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

Malavige

Hemalika

Chandrika

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: As secretory phospholipase A2 (sPLA2) was shown to be elevated in patients who progress to severe dengue, it would be important to evaluate the usefulness of therapeutics that inhibit sPLA2 enzymes to prevent progression to severe dengue. Methods: Aqueous and butanol extracts of Tragia hispida, Justicia adathoda and tubers of Cyperus rotundus were screened for the presence of potential sPLA2 inhibitors using a commercial assay measuring sPLA2 activity. Results: Both the aqueous (THA) and butanol extracts (THB) of Tragia hispida had sPLA2 inhibition levels comparable to the levels seen with the commercial sPLA2 inhibitor CAY10590. THB at concentrations of 0.1 µg/µL and 0.2 µg/µL, significantly reduced the sPLA2 activity (p<0.0001) in the sera of dengue patients and the inhibitory activity was significantly higher (p<0.0001) than of CAY10590. Thin layer chromatography of THB showed that it was likely to contain a mixture of flavonoid and phenolic compounds. HPLC displayed peaks at 3.207 min (λmax 222 nm, 272 nm) and 7.972 min (λmax 224 nm, 272 nm) which were most likely to represent phenolics and peaks at 11.883 min (λmax 276 nm, 366 nm) and 16.898 min ( λmax 254 nm, 370 nm) which were most likely to represent flavonoids. Conclusions:T. hispida aqueous and butanol soluble fraction had potent sPLA2 inhibitory activities, which should be further explored for their potential to be used for treatment of dengue.

show abstract

Tragia L. Genus: Ethnopharmacological Use, Phytochemical Composition and Biological Activity

Cited by 6 publications

References 77 publications

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

Characterization of secretory phospholipase A2 inhibitory activity inTragia hispidaas potential therapeutic agent for treatment of dengue

Characterization of secretory phospholipase A2 inhibitory activity in Tragia hispida as potential therapeutic agent for treatment of dengue

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