Trafficking SNARE SYP132 Partakes in Auxin-Associated Root Growth

Xia, Lingfeng; Marquès-Bueno, María Mar; Karnik, Rucha

doi:10.1104/pp.19.01301

Cited by 13 publications

(20 citation statements)

References 17 publications

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“…We suspected therefore that partial suppression of SYP132 , such as exhibited by the haplo-impaired syp132 T line, would likely be difficult to interpret. In p CaMV 35S: RFP-SYP132 Arabidopsis over-expressing RED FLUORESCENT PROTEIN (RFP)-tagged SYP132 (SYP132-OX; Xia et al, 2019 , 2020 ) the background SYP132 expression is almost 12-folds higher compared with the wild-type plants, thus opposing the natural order ( Xia et al, 2019 , 2020 ). Therefore, to study this SNARE during pathogenesis, we used SYP132-OX plants as a tool, and for comparing with wild-type and aha1-7 mutants.…”

Section: Resultsmentioning

confidence: 99%

“…In parallel, membrane traffic modulates the availability of these transporters at the plasma membrane for active function ( Hager, 2003 ; Hashimoto-Sugimoto et al, 2013 ; Xia et al, 2019 ). Recent studies demonstrate that SYNTAXIN OF PLANTS132 (SYP132), a soluble N -ethylamide-sensitive factor attachment protein receptor (SNARE) protein, promotes PM H + -ATPase traffic ( Xia et al, 2019 , 2020 ). Thereby, over-expression of the SYP132 reduces PM H + -ATPase density and suppresses plant growth ( Richardson, 2019 ; Xia et al, 2019 , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies demonstrate that SYNTAXIN OF PLANTS132 (SYP132), a soluble N -ethylamide-sensitive factor attachment protein receptor (SNARE) protein, promotes PM H + -ATPase traffic ( Xia et al, 2019 , 2020 ). Thereby, over-expression of the SYP132 reduces PM H + -ATPase density and suppresses plant growth ( Richardson, 2019 ; Xia et al, 2019 , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis

et al. 2022

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The vesicle trafficking SYNTAXIN OF PLANTS132 (SYP132) drives hormone-regulated endocytic traffic to suppress the density and function of plasma membrane H+-ATPases. In response to bacterial pathogens, it also promotes secretory traffic of antimicrobial pathogenesis-related (PR) proteins. These seemingly opposite actions of SYP132 raise questions about the mechanistic connections between the two, likely independent, membrane trafficking pathways intersecting plant growth and immunity. To study SYP132 and associated trafficking of plasma membrane H+-ATPase 1 (AHA1) and PATHOGENESIS-RELATED PROTEIN1 (PR1) during pathogenesis, we used the virulent Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) bacteria for infection of Arabidopsis (Arabidopsis thaliana) plants. SYP132 overexpression suppressed bacterial infection in plants through the stomatal route. However, bacterial infection was enhanced when bacteria were infiltrated into leaf tissue to bypass stomatal defences. Tracking time-dependent changes in native AHA1 and SYP132 abundance, cellular distribution, and function, we discovered that bacterial pathogen infection triggers AHA1 and SYP132 internalisation from the plasma membrane. AHA1 bound to SYP132 through its regulatory SNARE Habc domain, and these interactions affected plasma membrane H+-ATPase traffic. Remarkably, using the Arabidopsis aha1 mutant, we discovered that AHA1 is essential for moderating SYP132 abundance and associated secretion of PR1 at the plasma membrane for pathogen defence. Thus, we show that, during pathogenesis, SYP132 coordinates AHA1 with opposing effects on the traffic of AHA1 and PR1.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis

et al. 2022

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“…Auxin regulates SYP132 in root growth and the geotropic response. The expression of SYP132 is tightly regulated by auxin, and increased expression of SYP132 reduces the content of H + -ATPase protein on the plasma membrane and is involved in the endocytosis of plasma membrane H + -ATPase proteins, reducing their density and activity on the PM (Xia et al, 2019(Xia et al, , 2020. The loss of function of SYP123 and SYP132 leads to serious defects in root hair elongation.…”

Section: Pm-associated Snares In the Rootmentioning

confidence: 99%

SNAREs Regulate Vesicle Trafficking During Root Growth and Development

2022

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SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins assemble to drive the final membrane fusion step of membrane trafficking. Thus, SNAREs are essential for membrane fusion and vesicular trafficking, which are fundamental mechanisms for maintaining cellular homeostasis. In plants, SNAREs have been demonstrated to be located in different subcellular compartments and involved in a variety of fundamental processes, such as cytokinesis, cytoskeleton organization, symbiosis, and biotic and abiotic stress responses. In addition, SNAREs can also contribute to the normal growth and development of Arabidopsis. Here, we review recent progress in understanding the biological functions and signaling network of SNAREs in vesicle trafficking and the regulation of root growth and development in Arabidopsis.

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“…A total of 65, 70, and 151 SNARE genes are present in Arabidopsis, rice, and soybean, respectively (Won and Kim, 2020). By mediating vesicle fusion, SNAREs transport cargos and fulfill indispensable roles in plant development (Richardson, 2019; Xia et al ., 2019, 2020) and in innate immune responses (Pant et al ., 2015; Wang et al ., 2018; Zhu et al ., 2019). In addition, SNAREs serve as important regulatory factors in abiotic stress responses.…”

Section: Introductionmentioning

confidence: 99%

Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor‐like kinases CRCK1s to modulate salt stress responses

et al. 2020

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Summary Plants have evolved numerous receptor‐like kinases (RLKs) that modulate environmental stress responses. However, little is known regarding soybean (Glycine max) RLKs. We have previously identified that Glycine soja Ca2+/CAM‐binding RLK (GsCBRLK) is involved in salt tolerance. Here, we report that soluble NSF attachment protein receptor proteins BET1s mediate subcellular localization of calmodulin‐binding receptor‐like cytoplasmic kinases CRCK1s to modulate salt stress responses. Direct interaction between GsCBRLK and GsBET11a was initially identified via yeast two‐hybrid and bimolecular fluorescence complementation assays. Further analysis demonstrated conserved interaction between BET1s and CRCK1s. GsCBRLK interacted with all BET1 proteins in wild soybean (Glycine soja) and Arabidopsis, and GsBET11a strongly associated with GsCRCK1a–1d, but slightly with AtCRCK1. In addition, GsBET11a interacted with GsCBRLK via its C‐terminal transmembrane domain (TMD), where the entire TMD, not the sequence, was critical for the interaction. Moreover, the N‐terminal variable domain (VD) of GsCBRLK was responsible for interacting with GsBET11a, and the intensity of interaction between GsCBRLK/AtCRCK1 and GsBET11a was dependent on VD. Furthermore, GsBET11a was able to mediate the GsCBRLK subcellular localization via direct interaction with VD. Additionally, knockout of AtBET11 or AtBET12 individually did not alter GsCBRLK localization, while GsBET11a expression caused partial internalization of GsCBRLK from the plasma membrane (PM). We further suggest the necessity of GsCBRLK VD for its PM localization via N‐terminal truncation assays. Finally, GsBET11a was shown to confer enhanced salt stress tolerance when overexpressed in Arabidopsis and soybean. These results revealed the conserved and direct interaction between BET1s and CRCK1s, and suggested their involvement in salt stress responses.

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Trafficking SNARE SYP132 Partakes in Auxin-Associated Root Growth

Cited by 13 publications

References 17 publications

SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis

SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis

SNAREs Regulate Vesicle Trafficking During Root Growth and Development

Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor‐like kinases CRCK1s to modulate salt stress responses

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