Trafficking of cysteine proteinase to Leishmania lysosomes: lack of involvement of glycosylation

Aziridine-2,3-Dicarboxylate-Based Cysteine Cathepsin Inhibitors Induce Cell Death inLeishmania majorAssociated with Accumulation of Debris in Autophagy-Related Lysosome-Like Vacuoles

“…Similar phenotypes were also described by other groups for L. major and L. mexicana after treatment with cysteine cathepsin inhibitors (31,44) and resemble alterations seen in lysosomal storage diseases caused by the deficiency or absence of specific acidic hydrolases. Localization of cysteine cathepsins in lysosome-like vacuoles of L. major promastigotes was described by Boukai and coworkers (4). However, the lysosomal compartment is not only the end point of the endocytic pathway; it is also associated with the crucial autodegradative system, the autophagic pathway (35).…”

Special organelles of some pathogenic protozoa

“…Subsequently the protein is accumulated in the reservosomes (Fig. 24) (Huete-Pe´rez et al 1999;Boukai et al 2000).…”

Ultrastructural and cytochemical identification of megasome in Leishmania (Leishmania) chagasi

“…More recent data have described the antigenicity of Leishmania cysteine proteinases and their involvement with partially protective cellular immune responses against homologous infection (Wolfram et al 1995;Beydrot et al 1997;Rafati et al 2000Rafati et al , 2001. Cysteine proteinases associated with megasomes have been described in L. (L.) mexicana, L. (L.) amazonensis, and L. (L.) pifanoi (Pupkis et al 1986;Duboise et al 1994;Beyrodt et al 1997) and this close association between cysteine proteinase and megasome has provided a very suitable system for studies of processing and trafficking of cysteine proteinases in Leishmania (Boukai et al 2000;Brooks et al 2000;Costa-Pinto et al 2001). Visceralizing species of Leishmania present very low cysteine proteinase activity (Coombs 1982;Pinto et al 2000) and megasomes have not been reported in these species.…”