Trafficking in blood vessel development

Francis, Caitlin R.; Kushner, Erich J.

doi:10.1007/s10456-022-09838-5

Cited by 12 publications

(8 citation statements)

References 141 publications

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“…1) are dependent on unique cellular processes within the ECs themselves, e.g. protein trafficking, expression of proteases, cellular migration, proliferation and differentiation [26][27][28]. In tumors, several non-angiogenic mechanisms of vascularization have also been recognized and may operate in parallel to canonical angiogenesis mechanisms.…”

Section: Mechanisms Of Building a Vasculaturementioning

confidence: 99%

Pathological angiogenesis: mechanisms and therapeutic strategies

Dudley

2023

Angiogenesis

112

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In multicellular organisms, angiogenesis, the formation of new blood vessels from pre-existing ones, is an essential process for growth and development. Different mechanisms such as vasculogenesis, sprouting, intussusceptive, and coalescent angiogenesis, as well as vessel co-option, vasculogenic mimicry and lymphangiogenesis, underlie the formation of new vasculature. In many pathological conditions, such as cancer, atherosclerosis, arthritis, psoriasis, endometriosis, obesity and SARS-CoV-2(COVID-19), developmental angiogenic processes are recapitulated, but are often done so without the normal feedback mechanisms that regulate the ordinary spatial and temporal patterns of blood vessel formation. Thus, pathological angiogenesis presents new challenges yet new opportunities for the design of vascular-directed therapies. Here, we provide an overview of recent insights into blood vessel development and highlight novel therapeutic strategies that promote or inhibit the process of angiogenesis to stabilize, reverse, or even halt disease progression. In our review, we will also explore several additional aspects (the angiogenic switch, hypoxia, angiocrine signals, endothelial plasticity, vessel normalization, and endothelial cell anergy) that operate in parallel to canonical angiogenesis mechanisms and speculate how these processes may also be targeted with anti-angiogenic or vascular-directed therapies.

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Section: Mechanisms Of Building a Vasculaturementioning

confidence: 99%

Pathological angiogenesis: mechanisms and therapeutic strategies

Dudley

2023

Angiogenesis

112

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“…This is best illustrated by the dependency of critical receptors such as vascular endothelial growth factor receptor 2 (VEGFR2), or other receptor tyrosine kinase family members, on endocytic processes for activation [1][2][3][4]. Specifically, internalization of these receptors propagates a signaling response as well as timely quenching of receptor activity [5]. This removal of proteins from the plasma membrane is largely carried out by a well-characterized process termed clathrin-mediated endocytosis (CME) [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Arf6 Regulates Endocytosis and Angiogenesis by Promoting Filamentous Actin Assembly

Francis

Bell

Skripnichuk

et al. 2023

Preprint

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Clathrin-mediated endocytosis (CME) is a process vital to angiogenesis as well as general vascular homeostasis. In pathologies where supraphysiological growth factor signaling underlies disease etiology, such as in diabetic retinopathy and solid tumors, strategies to limit chronic growth factor signaling by way of CME have been shown to have tremendous clinical value. ADP ribosylation factor 6 (Arf6) is a small GTPase that promotes the assembly of actin necessary for CME. In its absence, growth factor signaling is greatly diminished, which has been shown to ameliorate pathological signaling input in diseased vasculature. However, it is less clear if there are bystander effects related to loss of Arf6 on angiogenic behaviors. Our goal was to provide a analysis of Arf6s function in angiogenic endothelium, focusing on its role in lumenogenesis as well as its relation to actin and CME. We found that Arf6 localized to both filamentous actin and sites of CME in 2-dimensional culture. Loss of Arf6 distorted both apicobasal polarity and reduced the total cellular filamentous actin content, and this may be the primary driver underlying gross dysmorphogenesis during angiogenic sprouting in its absence. Our findings highlight that endothelial Arf6 is a potent mediator of both actin regulation and CME.

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“…Similarly, no one has yet described any features in the molecular organization of EC transport routes. A review describing the intracellular transport during angiogenesis was published by Francis and Kushner [ 20 ]. Description and images of the GC in ECs are presented by Neumuller and Ellinger [ 21 ].…”

Section: Compartments Of Intracellular Transport In Ecsmentioning

confidence: 99%

“…Some CD63/lamp3 is also present in WPBs [ 40 ]. VWF and WPBs are used as markers of ECs in culture and in situ [ 20 ]. The tetraspanin CD63/lamp3 distributes predominantly to the internal membranes of late endosomes, which contain the unique lipid lyso-bis-phosphatidic acid, and cycles between endocytic and secretory compartments.…”

Section: Compartments Of Intracellular Transport In Ecsmentioning

confidence: 99%

Intracellular Membrane Transport in Vascular Endothelial Cells

Mirоnоv

Mironov

Sanavio

et al. 2023

IJMS

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The main component of blood and lymphatic vessels is the endothelium covering their luminal surface. It plays a significant role in many cardiovascular diseases. Tremendous progress has been made in deciphering of molecular mechanisms involved into intracellular transport. However, molecular machines are mostly characterized in vitro. It is important to adapt this knowledge to the situation existing in tissues and organs. Moreover, contradictions have accumulated within the field related to the function of endothelial cells (ECs) and their trans-endothelial pathways. This has induced necessity for the re-evaluation of several mechanisms related to the function of vascular ECs and intracellular transport and transcytosis there. Here, we analyze available data related to intracellular transport within ECs and re-examine several hypotheses about the role of different mechanisms in transcytosis across ECs. We propose a new classification of vascular endothelium and hypotheses related to the functional role of caveolae and mechanisms of lipid transport through ECs.

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Trafficking in blood vessel development

Cited by 12 publications

References 141 publications

Pathological angiogenesis: mechanisms and therapeutic strategies

Pathological angiogenesis: mechanisms and therapeutic strategies

Arf6 Regulates Endocytosis and Angiogenesis by Promoting Filamentous Actin Assembly

Intracellular Membrane Transport in Vascular Endothelial Cells

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