Traffic pathways of Plasmodium vivax antigens during intraerythrocytic parasite development

Guzmán-Bracho, Carmen; Dunia, I.; Rosa, Mercedes de la; Benedetti, E.L.; Pérez, H.

doi:10.1007/s00436-001-0537-8

Cited by 6 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Pv-148 is synthesized by P. vivax parasites throughout the asexual development, and it becomes exported into the host cell cytosol when reaching the surface membrane of the infected erythrocyte via clefts (Bracho et al 2002). Results showed that antiflotillin-2 co-labeled the clefts and the cell surface domains where Pv-148 was present (Fig.…”

Section: Resultsmentioning

confidence: 92%

See 1 more Smart Citation

Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

et al. 2006

Self Cite

View full text Add to dashboard Cite

Blood stages of Plasmodium vivax induce the development of caveolae and caveola-vesicle complexes (CVC) in the membrane of their host erythrocyte. Caveolae are found in almost all types of cells and are involved in endogenous processes as calcium and cholesterol homeostasis, cell signalling, transporting, ligand internalization and transcytosis of serum components. Major structural components of caveolae are the proteins caveolins and flotillins. The functional role of caveolae in the P. vivax-infected erythrocyte is not properly understood. As these organelles have been shown to contain malaria antigens, it has been suggested that they are involved in the transport and release of specific parasite antigens from the infected erythrocyte and in the uptake of plasma proteins. Using specific antibodies to classical caveolae proteins and an immunolocalization approach, we found caveolin-2, caveolin-3, and flotillin-2 in the vesicle profiles and some CVC of P. vivax-infected erythrocytes. Caveolin-1-3 were not found in uninfected erythrocytes. This is the first report of identification and localization of caveolins in the CVC present in erythrocytes infected with P. vivax, thereby providing evidence of the role of this particular organelle in the protein-trafficking pathway that connect parasite-encoded proteins with the erythrocyte cytoplasm and the cell surface throughout the asexual blood cycle of vivax malaria parasite.

show abstract

Section: Resultsmentioning

confidence: 92%

“…The IgG1 monoclonal antibody (mAb) raised against flotillin-2 of human origin was obtained from BD Biosciences (San Diego, CA, USA). The IgM 7C5B71 mAb which identifies the 148-kDa P. vivax (Pv-148) protein was produced previously in our laboratory (Perez et al 1997;Bracho et al 2002).…”

Section: Primary Antibodiesmentioning

confidence: 99%

Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

et al. 2006

Self Cite

View full text Add to dashboard Cite

show abstract

“…Blocking the assembly of the network, blocks the delivery of exogenous lucifer yellow. Findings suggest that the TVM is part of the secretory pathway involved in lipid transport across the Plasmodium infected erythrocyte 12 .…”

Section: Lipid Transport Mechanisms In Plasmodiummentioning

confidence: 97%

Lipid Transport in Plasmodium: Role as Possible Target for Novel Chemotherapy Against Malaria

Nwobodo¹,

Okonkwo²,

Igwe³

2011

Biosci., Biotechnol. Res. Asia

View full text Add to dashboard Cite

Lipid trafficking pathways in malaria-infected erythrocytes are complex because the malaria parasite is separated from the serum by the erythrocyte and parasitophorous vacuolar membrane (PVM). The PVM lipids in malaria-infected erythrocytes are derived from host cells. Lipid rafts which are cholesterol and sphingolipid enriched membrane domains appear to be involved in malaria infection. Thus, perturbation of lipid raft specific lipids in the host erythrocyte membrane can influence the cell's ability to be infected. This paper attempts to discuss novel approaches in the treatment of malaria infection by targeting and manipulating host cell lipids based on recent discoveries on the role of lipid rafts in malaria pathogenesis.

show abstract

“…Because the mature erythrocyte has no intracellular organelles and none of the trafficking machinery used by most eukaryotic cells to transfer proteins to their correct destinations, the parasite needs to set up a completely novel system for trafficking of proteins and antigens to the surface of red blood cells [3], [4]. This system includes insertion of membrane profiles in the cytoplasm of the erythrocyte, such as membrane clefts, named Maurer's clefts in P. falciparum [5] or membrane clefts in other species such as the human parasite P. vivax [6] and rodent malaria parasite P. berghei [7], and a tubovesicular network, a term that has been introduced for a membranous network formed by projections of the parasitophorous vacuole membrane (PVM), described in P. falciparum [8], P. vivax [6] and P. berghei [7]. An association between these membranous structures with alterations on the surface of infected erythrocyte has been demonstrated by Bhattacharjee et al [9].…”

Section: Introductionmentioning

confidence: 99%

Visualizing the 3D Architecture of Multiple Erythrocytes Infected with Plasmodium at Nanoscale by Focused Ion Beam-Scanning Electron Microscopy

Medeiros

Souza

Jiao³

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

Different methods for three-dimensional visualization of biological structures have been developed and extensively applied by different research groups. In the field of electron microscopy, a new technique that has emerged is the use of a focused ion beam and scanning electron microscopy for 3D reconstruction at nanoscale resolution. The higher extent of volume that can be reconstructed with this instrument represent one of the main benefits of this technique, which can provide statistically relevant 3D morphometrical data. As the life cycle of Plasmodium species is a process that involves several structurally complex developmental stages that are responsible for a series of modifications in the erythrocyte surface and cytoplasm, a high number of features within the parasites and the host cells has to be sampled for the correct interpretation of their 3D organization. Here, we used FIB-SEM to visualize the 3D architecture of multiple erythrocytes infected with Plasmodium chabaudi and analyzed their morphometrical parameters in a 3D space. We analyzed and quantified alterations on the host cells, such as the variety of shapes and sizes of their membrane profiles and parasite internal structures such as a polymorphic organization of hemoglobin-filled tubules. The results show the complex 3D organization of Plasmodium and infected erythrocyte, and demonstrate the contribution of FIB-SEM for the obtainment of statistical data for an accurate interpretation of complex biological structures.

show abstract

Traffic pathways of Plasmodium vivax antigens during intraerythrocytic parasite development

Cited by 6 publications

References 32 publications

Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax

Lipid Transport in Plasmodium: Role as Possible Target for Novel Chemotherapy Against Malaria

Visualizing the 3D Architecture of Multiple Erythrocytes Infected with Plasmodium at Nanoscale by Focused Ion Beam-Scanning Electron Microscopy

Contact Info

Product

Resources

About