Traditional Mongolian Medicine: history and status quo

Pitschmann, Anna; Purevsuren, Sodnomtseren; Obmann, Astrid; Natsagdorj, Damdinsuren; Gunbilig, Disan; Narantuya, Samdan; Kletter, Christa; Glasl, Sabine

doi:10.1007/s11101-013-9321-5

Cited by 27 publications

(18 citation statements)

References 21 publications

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“…Traditional Mongolian medicine has been strongly influenced by Ayurveda and Tibetan medicine and, relying on herbal recipes, it is still the main medical care source in Mongolia (Pitschmann et al ., ). Today, officials in Mongolia are striving to scientifically prove the effectiveness of these traditional preparations.…”

Section: Introductionmentioning

confidence: 97%

Quantitation of phenylpropanoids and iridoids in insulin‐sensitising extracts of Leonurus sibiricus L. (Lamiaceae)

Pitschmann

Zehl

Heiss

et al. 2015

Phytochemical Analysis

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Section: Introductionmentioning

confidence: 97%

Quantitation of phenylpropanoids and iridoids in insulin‐sensitising extracts of Leonurus sibiricus L. (Lamiaceae)

Pitschmann

Zehl

Heiss

et al. 2015

Phytochemical Analysis

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“…1) Several chemical constituents have been identified from bulbs and other organs, most of them explaining why L. pumilum is used in traditional Mongolian medicine to cure liver diseases. 2,3) Unfortunately, vegetative reproduction is difficult. Since bulb scales are tightly appressed to the bulb, propagation by scales is impractical.…”

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confidence: 99%

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Zhang

Gai

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Somatic embryogenesis and organogenesis in Lilium pumilum were successfully regulated by picloram, α-naphthaleneacetic acid (NAA), and 6-benzyladenine (BA). In organogenesis, the highest shoot regeneration frequency (92.5%) was obtained directly from bulb scales on Murashige and Skoog (MS) medium containing 2.0 mg L(-1) BA and 0.2 mg L(-1) NAA, while organogenic callus (OC) formed from leaves on MS medium supplemented with 1.0 mg L(-1) BA and 0.5 mg L(-1) NAA. Following subculture, 76.7% of OC regenerated shoots. In somatic embryogenesis, the combination of picloram and NAA increased the amount of embryogenic callus (EC) that formed with a maximum on 90.7% of all explants which formed 11 somatic embryos (SEs) per explant. Differences between EC and OC in cellular morphology and cell differentiation fate were easily observed. SEs initially formed via an exogenous or an endogenous origin. The appearance of a protoderm in heart-shaped SE and the bipolar shoot-root development in oval-shaped SE indicated true somatic embryogenesis. This protocol provides a new and detailed regulation and histological examination of regeneration pattern in L. pumilum.

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“…Our data indicate that various expression levels of LpABCB21 may also influence auxin transport rate. Several studies have shown that SE is more easily induced when the explants contain high auxin levels [ 50 , 51 , 52 ], which indirectly indicates that the auxin content in the explants reaches a high level in advance may promote earlier formation of somatic embryos. However, the ratio of auxin to cytokinin determines the development fate of the reproductive organs [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Improvement of a Genetic Transformation System and Preliminary Study on the Function of LpABCB21 and LpPILS7 Based on Somatic Embryogenesis in Lilium pumilum DC. Fisch

Song

Yan

Wang

et al. 2020

IJMS

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Auxin transport mediates the asymmetric distribution of auxin that determines the fate of cell development. Agrobacterium-mediated genetic transformation is an important technical means to study gene function. Our previous study showed that the expression levels of LpABCB21 and LpPILS7 are significantly up-regulated in the somatic embryogenesis (SE) of Lilium pumilum DC. Fisch. (L. pumilum), but the functions of both genes remain unclear. Here, the genetic transformation technology previously developed by our team based on the L.pumilum system was improved, and the genetic transformation efficiency increased by 5.7–13.0%. Use of overexpression and CRISPR/Cas9 technology produced three overexpression and seven mutant lines of LpABCB21, and seven overexpression and six mutant lines of LpPILS7. Analysis of the differences in somatic embryo induction of transgenic lines confirmed that LpABCB21 regulates the early formation of the somatic embryo; however, excessive expression level of LpABCB21 inhibits somatic embryo induction efficiency. LpPILS7 mainly regulates somatic embryo induction efficiency. This study provides a more efficient method of genetic transformation of L. pumilum. LpABCB21 and LpPILS7 are confirmed to have important regulatory roles in L. pumilum SE thus laying the foundation for subsequent studies of the molecular mechanism of Lilium SE.

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Traditional Mongolian Medicine: history and status quo

Cited by 27 publications

References 21 publications

Quantitation of phenylpropanoids and iridoids in insulin‐sensitising extracts of Leonurus sibiricus L. (Lamiaceae)

Quantitation of phenylpropanoids and iridoids in insulin‐sensitising extracts of Leonurus sibiricus L. (Lamiaceae)

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Improvement of a Genetic Transformation System and Preliminary Study on the Function of LpABCB21 and LpPILS7 Based on Somatic Embryogenesis in Lilium pumilum DC. Fisch

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