Traditional herbal formula Jakyakgamcho-tang (Paeonia lactiflora and Glycyrrhiza uralensis) impairs inflammatory chemokine production by inhibiting activation of STAT1 and NF-κB in HaCaT cells

Jeong, Soo‐Jin; Lim, Hye‐Sun; Seo, Chang‐Seob; Kim, Jung Hoon; Jin, Seong-Eun; Yoo, Sae‐Rom; Shin, Hyeun‐Kyoo

doi:10.1016/j.phymed.2014.12.002

Cited by 50 publications

(42 citation statements)

References 32 publications

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“…Activation of JNK/ERK/p38 and STAT-1/Jak-2 are closely associated with chemokine production in TNF-α/IFN-γ-stimulated HaCaT cells [8]. Therefore, we investigated whether WSR influences TNF-α/IFN-γ-stimulated HaCaT cells by Western blotting.…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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BackgroundSanguisorbae Radix (SR) is a well-known herbal medicine used to treat inflammatory disease and skin burns in Asia. In addition, it is used to treat many types of allergic skin diseases, including urticaria, eczema, and allergic dermatitis. SR has been reported to exhibit anti-wrinkle, anti-oxidant, and anti-contact dermatitis bioactivities.MethodsIn this study, we investigated the mechanism underlying the anti-inflammatory effects of SR water extract (WSR) using human keratinocyte (HaCaT) cells and BALB/c mouse bone marrow-derived mast cells (BMMCs). Viability assays were used to evaluate non-cytotoxic concentrations of WSR in both BMMCs and HaCaT cells. To investigate the effect of WSR treatment on the degranulation of IgE/Ag-activated BMMCs, we measured the release of β-hexosaminidase (β-HEX). We determined the production of pro-inflammatory chemokines including thymus and activation regulated chemokine (TARC; CCL17), regulated on activation, normal T-cell expressed and secreted (RANTES; CCL5), macrophage-derived chemokine (MDC; CCL22), and interleukin 8 (IL-8; CXCL8) in stimulated human keratinocytes. The ability of WSR to reduce the expression of pro-inflammatory marker proteins was evaluated by Western blotting in HaCaT cells stimulated with tumor necrosis factor (TNF)-α/interferon (IFN)-γ.ResultWSR inhibited IgE/Ag-activated mast cell degranulation in BMMCs. Treatment with various concentrations of WSR decreased β-HEX release in a dose-dependent manner with an IC50 of 27.5 μg/mL. In keratinocytes, WSR suppressed TNF-α/IFN-γ-induced chemokine production and pro-inflammatory molecules via a blockade STAT-1, Jak-2, p38, and JNK activation.ConclusionsThis results demonstrate that WSR inhibits degranulation of IgE/Ag-activated mast cells and inhibits the production of pro-inflammatory chemokines by suppressing the phosphorylation of p38 and JNK in HaCaT cells.

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Section: Resultsmentioning

confidence: 99%

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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“…NF‐κB, a family of transcription factors, is a key regulator in various inflammatory responses. It is activated through phosphorylation, and can be stimulated by a variety of stimuli, including lipopolysaccharide of gram‐negative bacteria . NF‐κB signaling can crosstalk with other inflammation‐related pathways and regulate cascades that contribute to periodontal damage .…”

mentioning

confidence: 99%

Mangiferin ameliorates Porphyromonas gingivalis‐induced experimental periodontitis by inhibiting phosphorylation of nuclear factor‐κB and Janus kinase 1–signal transducer and activator of transcription signaling pathways

Wang

Ding

et al. 2016

J of Periodontal Research

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“…Recent studies show that it has a potential as a therapeutic drug candidate for management of inflammatory skin diseases. It significantly attenuated TNF‐α and interferon‐γ‐mediated chemokine production in HaCaT keratinocytes and caused no cytotoxicity (Jeong et al, ). The main active ingredients glycyrrhetinic acid and paeoniflorin displayed a synergistic effect in transdermal pharmacokinetic/pharmacodynamic tests (Ding et al, ).…”

Section: Discussionmentioning

confidence: 99%

Herbal medicine for hand–foot syndrome induced by fluoropyrimidines: A systematic review and meta‐analysis

Deng

Sun

2018

Phytotherapy Research

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The aims of this study were to evaluate the efficacy of herbal medicine for the prevention and management of hand-foot syndrome (HFS) induced by fluoropyrimidines and to identify herbs associated with HFS alleviation for further research. The PubMed, Cochrane, Springer, China National Knowledge Infrastructure, and Wanfang databases were searched up to May 2017 for randomized controlled trials (RCTs) that evaluated herbal medicine for relieving HFS in patients undergoing fluoropyrimidine-based chemotherapy. Study evaluation and synthesis methods were in accordance with the Cochrane Handbook, and data were analyzed using RevMan 5.3. In total, 35 RCTs (2,668 participants) were included. Meta-analysis showed that the addition of herbal medicine significantly reduced the incidences of all-grade and high-grade HFS. The total effective rate and complete remission rate of HFS patients increased significantly with herbal medicine arm. Further sensitivity analysis identified Paeoniae Radix Alba, Carthami Flos, Cinnamomi Ramulus, and Glycyrrhizae Radix et Rhizoma as being consistently associated with significant reductions in HFS incidence without important heterogeneity. However, the lack of blinding in most studies may have led to overestimation of these effects. More high-quality RCTs and experimental research are needed to confirm and investigate the efficacy of the herbs identified in this study.

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Traditional herbal formula Jakyakgamcho-tang (Paeonia lactiflora and Glycyrrhiza uralensis) impairs inflammatory chemokine production by inhibiting activation of STAT1 and NF-κB in HaCaT cells

Cited by 50 publications

References 32 publications

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Mangiferin ameliorates Porphyromonas gingivalis‐induced experimental periodontitis by inhibiting phosphorylation of nuclear factor‐κB and Janus kinase 1–signal transducer and activator of transcription signaling pathways

Herbal medicine for hand–foot syndrome induced by fluoropyrimidines: A systematic review and meta‐analysis

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