Traditional Chinese Medicine Formula Kang Shuai Lao Pian Improves Obesity, Gut Dysbiosis, and Fecal Metabolic Disorders in High-Fat Diet-Fed Mice

Shouliang, Gong; Ye, Tingting; Wang, Meixia; Wang, Mengying; Li, Yufei; Ma, Lina; Yang, Yuhang; Wang, Yi; Zhao, Xiaoping; Liu, Li; Yang, Min; Chen, Huan; Qian, Jing

doi:10.3389/fphar.2020.00297

Cited by 50 publications

(35 citation statements)

References 60 publications

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“…The depletion of intestinal Lactobacillus is frequently associated with the disease. Oscillibacter, a beneficial bacterium, is significantly reduced in patients (Fang et al, 2016), piglets with intrauterine growth retardation (Zhang et al, 2019), and obese mice (Gong et al, 2020), indicating that the intestinal microflora constitution was disturbed. Ruminococcaceae is significantly higher in the intestinal flora of a high-risk colorectal cancer population than in a low-risk population (Moore and Moore, 1995).…”

Section: Discussionmentioning

confidence: 99%

Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

Wen

Wang

et al. 2021

Front. Microbiol.

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BackgroundHeat stress has negative effects on the intestinal health of humans and animals. However, the impact of heat stress on intestinal microbial and metabolic changes remains elusive. Here, we investigated the cecal microbial and metabolic profiles in mice in response to heat stress.MethodsThe mouse heat stress model was constructed by simulating a high-temperature environment. Twenty mice were randomly assigned to two groups, the control group (CON, 25°C) and the heat treatment group (HS, 40°C from 13:00 to 15:00 every day for 7 days). Serum and cecal contents were collected from the mice for serum biochemical analysis, 16S rRNA high-throughput sequencing, and non-targeted metabolomics.ResultsBoth core body temperature and water intake were significantly increased in the HS group. Serum biochemical indicators were also affected, including significantly increased triglyceride and decreased low-density lipoprotein in the heat stress group. The composition and structure of intestinal microbiota were remarkably altered in the HS group. At the species level, the relative abundance of Candidatus Arthromitus sp. SFB-mouse-Japan and Lactobacillus murinus significantly reduced, while that of Lachnospiraceae bacterium 3-1 obviously increased after HS. Metabolomic analysis of the cecal contents clearly distinguished metabolite changes between the groups. The significantly different metabolites identified were mainly involved in the fatty acid synthesis, purine metabolism, fatty acid metabolism, cyanoamino acid metabolism, glyceride metabolism, and plasmalogen synthesis.ConclusionIn summary, high temperature disrupted the homeostatic balance of the intestinal microbiota in mice and also induced significant alterations in intestinal metabolites. This study provides a basis for treating intestinal disorders caused by elevated temperature in humans and animals and can further formulate nutritional countermeasures to reduce heat stress-induced damage.

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Section: Discussionmentioning

confidence: 99%

Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

Wen

Wang

et al. 2021

Front. Microbiol.

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“…These families include Celastraceae, Zingiberaceae, Theaceae, Magnoliaceae, and Solanaceae ( 88 ). Traditional Chinese medicine delivers unique solutions to treat obesity, such as regulating fat metabolism, enhancing hormone level, regulating intestinal microflora, among other pathways ( 89 ). These findings are helpful for selection of herbal medicine or traditional Chinese medicine for further research.…”

Section: Therapeutics Of Obesitymentioning

confidence: 99%

Obesity: Epidemiology, Pathophysiology, and Therapeutics

2021

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Obesity is a complex multifactorial disease that accumulated excess body fat leads to negative effects on health. Obesity continues to accelerate resulting in an unprecedented epidemic that shows no significant signs of slowing down any time soon. Raised body mass index (BMI) is a risk factor for noncommunicable diseases such as diabetes, cardiovascular diseases, and musculoskeletal disorders, resulting in dramatic decrease of life quality and expectancy. The main cause of obesity is long-term energy imbalance between consumed calories and expended calories. Here, we explore the biological mechanisms of obesity with the aim of providing actionable treatment strategies to achieve a healthy body weight from nature to nurture. This review summarizes the global trends in obesity with a special focus on the pathogenesis of obesity from genetic factors to epigenetic factors, from social environmental factors to microenvironment factors. Against this background, we discuss several possible intervention strategies to minimize BMI.

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“…Kang-Shuai-Lao-Pian was found to affect the numbers of Intestinimonas, Oscillibacter, Christensenellaceae_R-7_group, and Lachnoclostridium_UCG-010, Aliihoeflea as well as regulate lysine, dipeptide, fatty acid and purine metabolism in obese mice (Gong et al, 2020).…”

Section: Introductionmentioning

confidence: 96%

Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism

Cui

Wang

et al. 2020

Front. Pharmacol.

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The dysbiosis in gut microbiota could affect host metabolism and contribute to the development of nonalcoholic fatty liver disease (NAFLD). Da-Chai-Hu decoction (DCH) has demonstrated protective effects on NAFLD, however, the exact mechanisms remain unclear. In this study, we established a NAFLD rat model using a high fat diet (HFD) and provided treatment with DCH. The changes in gut microbiota post DCH treatment were then investigated using 16S rRNA sequencing. Additionally, serum untargeted metabolomics were performed to examine the metabolic regulations of DCH on NAFLD. Our results showed that DCH treatment improved the dyslipidemia, insulin resistance (IR) and ameliorated pathological changes in NAFLD model rats. 16S rRNA sequencing and untargeted metabolomics showed significant dysfunction in gut microbiota community and serum metabolites in NAFLD model rats. DCH treatment restored the dysbiosis of gut microbiota and improved the dysfunction in serum metabolism. Correlation analysis indicated that the modulatory effects of DCH on the arachidonic acid (AA), glycine/serine/threonine, and glycerophospholipid metabolic pathways were related to alterations in the abundance of Romboutsia, Bacteroides, Lactobacillus, Akkermansia, Lachnoclostridium and Enterobacteriaceae in the gut microflora. In conclusion, our study revealed the ameliorative effects of DCH on NAFLD and indicated that DCH’s function on NAFLD may link to the improvement of the dysbiosis of gut microbiota and the modulation of the AA, glycerophospholipid, and glycine/serine/threonine metabolic pathways.

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Traditional Chinese Medicine Formula Kang Shuai Lao Pian Improves Obesity, Gut Dysbiosis, and Fecal Metabolic Disorders in High-Fat Diet-Fed Mice

Cited by 50 publications

References 60 publications

Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

Obesity: Epidemiology, Pathophysiology, and Therapeutics

Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism

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