Traditional Chinese medicine extraction method by ethanol delivers drug-like molecules

Miao, William Gang; Tang, Chunping; Yang, Ye; Quinn, Ronald J.; Ying, Feng

doi:10.1016/s1875-5364(19)30086-x

Cited by 10 publications

(11 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used the extraction method for Traditional Chinese medicine according to Ref. (Miao et al, 2019), with slight modification. The PML was first put into a decoction pot and soaked in distilled water for 20 min.…”

Section: Water Decoction Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Mulberry (Morus alba L.) Leaf Extracts on Growth, Immune Response, and Antioxidant Functions in Nile Tilapia (Oreochromis niloticus)

Tang

Gao

et al. 2022

Annals of Animal Science

View full text Add to dashboard Cite

This study evaluates how white mulberry (Morus alba L.) leaf extracts affect the growth, antioxidant activity, and immune response in Nile tilapia Oreochromis niloticus. Mulberry leaf extracts were obtained through aqueous extraction (AE) and ethanol extraction (EE). Powder of mulberry leaf (PML) was added directly to feed and compared with the effects of feeds supplemented with the different extracts. Fish were divided into eight groups for an 8-week feeding trial where they were fed the basal diet or supplementation with 10% PML, 10% AE, 20% AE, 40% AE, 10% EE, 20% EE, or 40% EE. The inclusion of mulberry leaf extract obtained with either method showed better effects on fish growth performance, antioxidant activities and acid phosphatase activity (ACP) in serum, immune cytokine expression, and intestinal morphology as compared with controls or fish fed the 10% PML diet. The specific growth rate was significantly higher in the 10% AE, 10% EE, and 20% EE groups compared with all other groups (P<0.05). Catalase activity was significantly greater in most groups fed an extract, and in the 10% PML group, when compared with controls. Similarly, ACP, interleukin (IL)-1, and IL-2 expression was significantly increased in groups fed an extract, and in the 10% PML group, when compared with controls (P<0.05). IL-1, IL-2, IL-10, and Toll-like receptor 2 expression was significantly greater in the 10% EE group than in the 10% PML and 10% AE groups (P<0.05). Villus length in the middle intestine was significantly increased in the 10% AE and 10% EE groups compared with controls and the 10% PML group (P<0.05). Thus, 10% mulberry leaf ethanol extract added to feed is recommended for enhancing the growth rate and health of cultured Nile tilapia.

show abstract

Section: Water Decoction Methodsmentioning

confidence: 99%

“…This is the traditional extraction method for traditional Chinese medicine according to Ref. (Miao et al, 2019) with slight modification. The PML was soaked in 70% ethanol (Guangzhou Hongzhou Chemical Co., Ltd.) and then slowly heated to 70℃ for 2 h; the liquid was poured through filter paper.…”

Section: Ethanol Extraction Methodsmentioning

confidence: 99%

Effects of Mulberry (Morus alba L.) Leaf Extracts on Growth, Immune Response, and Antioxidant Functions in Nile Tilapia (Oreochromis niloticus)

Tang

Gao

et al. 2022

Annals of Animal Science

View full text Add to dashboard Cite

show abstract

“…The decoctions were concentrated to 0.2 g/ml. The supernatant was collected as research objects after centrifugation, filtration, and sterilization ( Chen et al, 2019 ; Miao et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…Preparation of Qi-Invigorating Herbs All the Qi-invigorating herbs, including Ginseng Radix et Rhizoma, Codonopsis Radix, Pseudostellariae Radix, Astragali Radix, Glycyrrhizae Radix et Rhizoma, were purchased from the TCM pharmacy of Fujian Medical University Union Hospital, Fujian, China. Each herb (10 g) was placed in a beaker, then boiled water (100 ml) was added and the solution was kept boiling for 3 hours (Chen et al, 2019;Miao et al, 2019). The decoctions were concentrated to 0.2 g/ml.…”

Section: Performing Experimental Verificationmentioning

confidence: 99%

Impact of Qi-Invigorating Traditional Chinese Medicines on Diffuse Large B Cell Lymphoma Based on Network Pharmacology and Experimental Validation

et al. 2021

View full text Add to dashboard Cite

Background: It has been verified that deficiency of Qi, a fundamental substance supporting daily activities according to the Traditional Chinese Medicine theory, is an important symptom of cancer. Qi-invigorating herbs can inhibit cancer development through promoting apoptosis and improving cancer microenvironment. In this study, we explored the potential mechanisms of Qi-invigorating herbs in diffuse large B cell lymphoma (DLBCL) through network pharmacology and in vitro experiment.Methods: Active ingredients of Qi-invigorating herbs were predicted from the Traditional Chinese Medicine Systems Pharmacology Database. Potential targets were obtained via the SwissTargetPrediction and STITCH databases. Target genes of DLBCL were obtained through the PubMed, the gene-disease associations and the Malacards databases. Overlapping genes between DLBCL and each Qi-invigorating herb were collected. Hub genes were subsequently screened via Cytoscape. The Gene Ontology and pathway enrichment analyses were performed using the DAVID database. Molecular docking was performed among active ingredients and hub genes. Hub genes linked with survival and tumor microenvironment were analyzed through the GEPIA 2.0 and TIMER 2.0 databases, respectively. Additionally, in vitro experiment was performed to verify the roles of common hub genes.Results: Through data mining, 14, 4, 22, 22, 35, 2, 36 genes were filtered as targets of Ginseng Radix et Rhizoma, Panacis Quinquefolii Radix, Codonopsis Radix, Pseudostellariae Radix, Astragali Radix, Dioscoreae Rhizoma, Glycyrrhizae Radix et Rhizoma for DLBCL treatment, respectively. Then besides Panacis Quinquefolii Radix and Dioscoreae Rhizoma, 1,14, 10, 14,13 hub genes were selected, respectively. Molecular docking studies indicated that active ingredients could stably bind to the pockets of hub proteins. CASP3, CDK1, AKT1 and MAPK3 were predicted as common hub genes. However, through experimental verification, only CASP3 was considered as the common target of Qi-invigorating herbs on DLBCL apoptosis. Furthermore, the TIMER2.0 database showed that Qi-invigorating herbs might act on DLBCL microenvironment through their target genes. Tumor-associated neutrophils may be main target cells of DLBCL treated by Qi-invigorating herbs.Conclusion: Our results support the effects of Qi-invigorating herbs on DLBCL. Hub genes and immune infiltrating cells provided the molecular basis for each Qi-invigorating herb acting on DLBCL.

show abstract

“…However, people, especially Asians, are fascinated with the medicinal values of these woody mushrooms and have been using them traditionally for remedy or treatment of various ailments [ 1 ]. In ancient times, the wild fruiting body of Ganoderma was prepared as a tonic, tincture in alcohol, or decoction in water for strengthening body resistance, improving eyesight and vital essence and also nourishing spleen and liver [ 2 ]. Over centuries, the Ganoderma mushroom has been historically accredited as “Mushroom of Immortality”, and it is believed it can enhance health, well-being, and longevity [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity

Chan

Chong

2022

Molecules

View full text Add to dashboard Cite

Some species of Ganoderma, such as G. lucidum, are well-known as traditional Chinese medicine (TCM), and their pharmacological value was scientifically proven in modern days. However, G. boninense is recognized as an oil palm pathogen, and its biological activity is scarcely reported. Hence, this study aimed to investigate the antibacterial properties of G. boninense fruiting bodies, which formed by condensed mycelial, produced numerous and complex profiles of natural compounds. Extract was cleaned up with normal-phase SPE and its metabolites were analyzed using liquid chromatography–mass spectrometry (LCMS). From the disc diffusion and broth microdilution assays, strong susceptibility was observed in methicillin-resistant Staphylococcus aureus (MRSA) in elute fraction with zone inhibition of 41.08 ± 0.04 mm and MIC value of 0.078 mg mL−1. A total of 23 peaks were detected using MS, which were putatively identified based on their mass-to-charge ratio (m/z), and eight compounds, which include aristolochic acid, aminoimidazole ribotide, lysine sulfonamide 11v, carbocyclic puromycin, fenbendazole, acetylcaranine, tigecycline, and tamoxifen, were reported in earlier literature for their antimicrobial activity. Morphological observation via scanning electron microscope (SEM), cell membrane permeability, and integrity assessment suggest G. boninense extract induces irreversible damage to the cell membrane of MRSA, thus causing cellular lysis and death.

show abstract

Traditional Chinese medicine extraction method by ethanol delivers drug-like molecules

Cited by 10 publications

References 45 publications

Effects of Mulberry (Morus alba L.) Leaf Extracts on Growth, Immune Response, and Antioxidant Functions in Nile Tilapia (Oreochromis niloticus)

Effects of Mulberry (Morus alba L.) Leaf Extracts on Growth, Immune Response, and Antioxidant Functions in Nile Tilapia (Oreochromis niloticus)

Impact of Qi-Invigorating Traditional Chinese Medicines on Diffuse Large B Cell Lymphoma Based on Network Pharmacology and Experimental Validation

Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity

Contact Info

Product

Resources

About