Traction force and its regulation during cytokinesis in Dictyostelium cells

Jahan, Md. Golam Sarowar; Yumura, Shigehiko

doi:10.1016/j.ejcb.2017.06.004

Cited by 18 publications

(29 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypo-osmolarity and mechanical stretch also are activators of TRPV4 [30]. Given that cells generate traction force during division [19] and the mechanically gated ion channel Piezo 1 can respond to such force in neural stem cells [36], TRPV4 may be open while OPCs are dividing and increase their proliferation. TRPV4 is also activated by white matter components.…”

Section: Discussionmentioning

confidence: 99%

TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation

Ohashi

Deyashiki

Miyake

et al. 2018

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Oligodendrocytes, which differentiate from oligodendrocyte precursor cells (OPCs), ensheath axons with myelin, play an essential role in rapid conduction of action potentials and metabolically support neurons. Elucidation of the mechanisms underlying the proliferation, migration, differentiation, and survival of OPCs is considered indispensable for determining the causes of central nervous system diseases. However, the relationship between these functions of OPCs and their intracellular Ca signaling has not been fully elucidated. Here, we investigated the function of transient receptor potential vanilloid 4 (TRPV4), a Ca-permeable channel that responds to hypo-osmolarity, mild temperature, mechanical stimulation, and endogenous arachidonic acid metabolites, in OPCs. Trpv4 mRNA was detected in OPCs in vivo and in primary cultured rat OPCs. In Ca imaging experiments, treatment with the selective TRPV4 agonist GSK1016790A induced sustained elevation of the intracellular Ca concentration in OPCs in a concentration-dependent manner, which was almost completely suppressed by co-treatment with the selective TRPV4 antagonist HC067047. Stimulation of TRPV4 by GSK1016790A augmented OPC proliferation, which was abolished by co-treatment with HC067047, the intracellular Ca chelator BAPTA-AM, and the protein kinase C inhibitor bisindolylmaleimide II. By contrast, GSK1016790A did not significantly affect the migration or differentiation of OPCs. Taken together, these results suggest that TRPV4 is functionally expressed in OPCs and increases the proliferation of these cells without affecting their ability to differentiate into oligodendrocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation

Ohashi

Deyashiki

Miyake

et al. 2018

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…To normalize the cell division stage, MSI was used as described previously (Jahan and Yumura, 2017). MSI was computed from the long axis (L) and short axis (l), where the short axis represents the width of the furrow, using the following formula:…”

Section: Microscopymentioning

confidence: 99%

“…The thickness of the cells under the agar overlay remained at about 2 µm during cell division ( Figure 1F). Because the division time varied between cells, we used the mitosis stage index (MSI; calculated from the long axis and short axis) to normalize the cell division time (Jahan and Yumura, 2017). When the MSI is 0, the cell shape is round, corresponding to the metaphase; when the MSI is 1, the cell separates into two daughter cells.…”

Section: Total Cell Surface Area During Cell Divisionmentioning

confidence: 99%

“…As described above, membrane surface area increase is necessary for cytokinesis; we therefore examined whether cytokinesis is required for the membrane surface area increase in Dictyostelium. Myosin II null (HS1) cells can divide by binary fission in surface culture depending on the opposite traction forces of the daughter halves (Neujahr et al, 1997;Jahan and Yumura, 2017). However, when cultured in suspension, the cell size increases and multiple nuclei are formed by normal nuclear division accompanying spindle formation (Zang et al, 1997;Taira and Yumura, 2017).…”

Section: Proper Furrowing Is Required For the Surface Area Increasementioning

confidence: 99%

See 1 more Smart Citation

Regulation of the Total Cell Surface Area in Dividing Dictyostelium Cells

Tanaka

Fujimoto

Yumura

2020

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

When a cell divides into two daughter cells, the total cell surface area should increase. There are two models for membrane supply to support cell division: (1) unfolding of small surface membrane reservoirs such as microvilli or wrinkles and (2) exocytosis of intracellular vesicles. Here, we precisely measured the total cell surface area in dividing Dictyostelium cells, flattened by the agar overlay that eliminated the complexity of unfolding surface membrane reservoirs. Because the cells divided normally under the agar overlay, unfolding of surface membrane reservoirs was not required for cell division. Under the agar overlay, the total cell surface area slightly decreased from the interphase to the metaphase and then increased about 20% during cytokinesis. Both endocytosis and exocytosis were suppressed in the early mitotic phase but recovered during cytokinesis. The imbalance of endocytosis and exocytosis could contribute to the changes observed in the cell surface area. Clathrin-dependent endocytosis was also substantially suppressed during cytokinesis, but contrary to previous reports in cultured animal cells, it did not significantly contribute to the regulation of the cell surface area. Furrowing during cytokinesis was indispensable for the cell membrane increase, and vice versa.

show abstract

“…Organisms from two of the five eukaryotic supergroups (Burki, 2014), Amoebozoa and Opisthokonta (which includes fungi and animals), assemble an actin cytoskeleton-and myosin motor protein-based contractile ring, or 'cytokinetic ring' (CR), to accomplish cytokinesis (see Box 1) (Gu and Oliferenko, 2015;Willet et al, 2015;Balasubramanian, 2016;Srivastava et al, 2016;Bhavsar-Jog and Bi, 2017;Glotzer, 2017;Hardin et al, 2017;Jahan and Yumura, 2017), whereas the other supergroups rely on alternative mechanisms, such as other cytoskeletal proteins, motility-based mechanisms, or vesicle trafficking of membrane and cell wall to the division site (Farr and Gull, 2012;Hardin et al, 2017;Smertenko, 2018;Müller, 2019). This Review focuses on advances in understanding CR-mediated cytokinesis.…”

Section: Introductionmentioning

confidence: 99%

Molecular form and function of the cytokinetic ring

Mangione

Gould

2019

Journal of Cell Science

View full text Add to dashboard Cite

Animal cells, amoebas and yeast divide using a force-generating, actin-and myosin-based contractile ring or 'cytokinetic ring' (CR). Despite intensive research, questions remain about the spatial organization of CR components, the mechanism by which the CR generates force, and how other cellular processes are coordinated with the CR for successful membrane ingression and ultimate cell separation. This Review highlights new findings about the spatial relationship of the CR to the plasma membrane and the arrangement of molecules within the CR from studies using advanced microscopy techniques, as well as mechanistic information obtained from in vitro approaches. We also consider advances in understanding coordinated cellular processes that impact the architecture and function of the CR.

show abstract

Traction force and its regulation during cytokinesis in Dictyostelium cells

Cited by 18 publications

References 63 publications

TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation

TRPV4 is functionally expressed in oligodendrocyte precursor cells and increases their proliferation

Regulation of the Total Cell Surface Area in Dividing Dictyostelium Cells

Molecular form and function of the cytokinetic ring

Contact Info

Product

Resources

About