Tract microflora in Saudi patients with cholelithiasis

Harbi, Mamdouh Al; Osoba, Abimbola O.; Mowallad, Abdulfattah; Alahmadi, Khaled Mohammad

doi:10.1046/j.1365-3156.2001.00748.x

Cited by 25 publications

(34 citation statements)

References 13 publications

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“…The predominant bacterium according to AlHarbi et al [6] is E.Coli while Wayne and Whelan [7] reported Klebsiella as predominant organism in the biliary tract. Other organisms found in the bile include: Hence the various studies done on bacterial flora of biliary tract present a confusing picture, the present study has been aimed at evaluating the bacteriological profile, both aerobic and anaerobic of patients undergoing cholecystectomy and to proceed accordingly.…”

Section: Spreading Infection Via Lymphaticsmentioning

confidence: 97%

Bacteriological Profile of Bile in Patients Undergoing Cholecystectomy

Gill¹,

Sandhu²,

Luna³

et al. 2016

SAJB

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Cholecystitis and cholelithiasis are frequently met surgical conditions in North India. The presence of bacteria in bile at the time of surgery or invasive diagnostic procedures predisposes to complications like septicaemia. Aim of this study was to study the bacteriological profile -aerobic & anaerobic in the bile of patients undergoing cholecystectomy. The present study included 50 patients admitted in the department of general surgery undergoing cholecystectomy with preoperative diagnosis of biliary tract disease. Details of the operative findings were recorded. The bile from gallbladder was cultured for the presence of aerobic and anaerobic bacteria. The bacteriological examination of the bile yielded positive culture in 16 out of 50 cases (32%). Aerobic bactobilia was seen in 15 out of 16 patients (93.75%). Anaerobic bactobilia was seen in 4 out of 16 patients (25%). The infection was mostly due to single microorganism. In 9 out of 16 cases (56.25%) the growth was purely aerobic. E.Coli was the most commonly isolated aerobic bacteria (36%).

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Section: Spreading Infection Via Lymphaticsmentioning

confidence: 97%

Bacteriological Profile of Bile in Patients Undergoing Cholecystectomy

Gill¹,

Sandhu²,

Luna³

et al. 2016

SAJB

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“…The most commonly involved age group for cholelithiasis (32.5%) is found to be 30-39 years with a female predominance (M: F=1:3.2). 6 Taher MA et al did a prospective study in 75 patients with gallstone (66 females and 9 males). The ratio of females: males was (7.3:1).…”

Section: Age and Sex Prevalencementioning

confidence: 99%

Microflora of gall bladder bile in patients undergoing laparoscopic cholecystectomy

Prabhu¹,

Chandan²,

Sudarsan³

2018

Int Surg J

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Background: Gallstone disease is one of the most common problem affecting the digestive tract. The conditions that predispose to the development of gallstones are obesity, pregnancy, dietary factors, Crohn's disease, gastric surgery, terminal resection, hereditary spherocytosis, sickle cell disease, and thalassemia. The incidence is four times higher in women than in men with high prevalence among the age group of 30-40 years. Bacteria are commonly found in inflamed gallbladder and in patients with cholelithiasis, whereas evidence suggests that normal bile is sterile. So, exploring and identifying the microflora of gallbladder bile helps to choose appropriate antibiotics. Methods: Minimum of 50 Patients admitted under General Surgery, who are scheduled to undergo laparoscopic cholecystectomy. Five ml of bile will be aspirated from the gall bladder during laparoscopic cholecystectomy. The samples will be transferred onto a plain sterile container and then transferred at room temperature in an hour to the laboratory for culture. Results: In the present study 50 patients were evaluated for culture and gall bladder bile. Out of which maximum number of patients were clustered between 31-40 years. In the present study out of 50 patients, 34 (68%) were females and 16 (32%) were males. In the present study We found total of 33 (66%) yellow stones, 13 (26%) were black in colour and 4(8%) were brown stones. In this study bile culture was showing growth of the following organisms. The commonest organism was E. coli (64%) followed by Klebsiella (25%) and Enterococcus (11%). Conclusions: Bactibilia has long been known to be associated with biliary tract diseases and culturable bacteria in bile can represent a state of asymptomatic bactibilia which can disseminate after any intervention causing infective complication. Exploring the microflora of gall bladder bile important role in choosing the appropriate antibiotic to prevent complications.

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“…and Enterobacter spp (3). So far, the most common bacteria isolated from gallstones are aerobes (3,4), which include E. coli (5)(6)(7), Klebsiella spp. (6,7), Enterobacter fecalis (8,9), Streptococcus spp., Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

“…(6,7), Enterobacter fecalis (8,9), Streptococcus spp., Pseudomonas spp. (4,8,9) and Salmonella (10). However, anaerobes were also isolated in few cases (4) such as Clostridium spp., Bacteroids fragilis and Propioni bacteria (1,11).…”

Section: Introductionmentioning

confidence: 99%

“…(4,8,9) and Salmonella (10). However, anaerobes were also isolated in few cases (4) such as Clostridium spp., Bacteroids fragilis and Propioni bacteria (1,11). Infection was found to increase with age (> 60 years) and certain diseases like acute cholecystitis, but sex and the number of common bile duct stones had no influence on positive cultures (11).…”

Section: Introductionmentioning

confidence: 99%

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Urease Positive and Slime Producing Bacterial Activity: Results in Gallstone Precipitation and Solidification

Sharma

Sachan

Sharma

2013

Arch Clin Infect Dis

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Background: Bacteria are commonly isolated from gallstones. Presence of bacteria in gallstones might play a role in their formation. Objectives: We aimed to study different properties of isolates from gallstones and their relationship with the mechanism of gallstone formation. Patients and Methods:This study included 526 subjects. Gallstones and bile samples of the patients were cultured. Isolates were identified by different biochemical tests and examined for their β-glucuronidase activity, slime production and Urease activity. Biostatistical analyses were done to determine the relationship between the biliary bacterial factors and the types of gallstones. Role of Slime and urease activity in mechanism of gallstone formation were tested in vitro. Results: Overall, 94.77% of the gallstones were found to be infected, of which 85% of the cholesterol stones, 97% of pigmented stones and 100% of mixed stones showed the presence of bacterial isolates (P < 0.0001). β-glucuronidase activity was associated with isolates from pigmented or mixed stones and completely absent in cholesterol gallstones (P = 0.018). Urease activity was basically associated with cholesterol gallstones (P < 0.0001) while slime (P < 0.0001) activity was not specific for any single type of stone formation yet isolates of bile had a significant association with slime activity. In vitro analysis demonstrated that bacteria with urease activity are involved in CaCO 3 precipitation and slime-producing isolates solidify the precipitated CaCO 3 . Conclusions: β-glucuronidase activity is mainly responsible for pigmented gallstone formation. Urease activity was predicted to be involved in nucleation and slime production during solidification of gallstones, as observed during in vitro studies. Hence, presence of these bacterial factors may enhance gallstone formation.

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Tract microflora in Saudi patients with cholelithiasis

Cited by 25 publications

References 13 publications

Bacteriological Profile of Bile in Patients Undergoing Cholecystectomy

Bacteriological Profile of Bile in Patients Undergoing Cholecystectomy

Microflora of gall bladder bile in patients undergoing laparoscopic cholecystectomy

Urease Positive and Slime Producing Bacterial Activity: Results in Gallstone Precipitation and Solidification

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