Tracking verocytotoxigenic Escherichia coli O157, O26, O111, O103 and O145 in Irish cattle

Thomas, Kate M.; McCann, M.S.; Collery, Mark M.; Logan, Anna; Whyte, Paul; McDowell, D.A.; Duffy, Geraldine

doi:10.1016/j.ijfoodmicro.2011.11.012

Cited by 65 publications

(54 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was noteworthy that the proportion of STEC among the recovered E. coli strains depended on the targeted serotype and was high for serotype O157:H7 but much lower for serotypes O26:H11 and O103:H2. These results are in agreement with previous studies evaluating stx carriage in top five serogroups of E. coli strains isolated from Irish beef slaughter chains and Scottish and Swiss cattle (14,16,43). Whether these results reflect a higher stability of stx bacteriophages within strains of serotype O157:H7 remains to be investigated.…”

Section: Discussionsupporting

confidence: 92%

“…Concerning the non-O157:H7 STEC serotypes, prevalence data directly comparable to our results are lacking in the literature. Recent studies focused on the detection of the top five STEC in cattle feces, but none of them led to the estimation of their prevalence, due either to a limited number of serogroup-specific strain isolations performed (14,15) or to a sampling strategy that did not allow a national estimation of the prevalence (16,18). In our study, the four non-O157:H7 STEC serotypes were detected in slaughtered categories at a low prevalence, ranging from 0.0% to 1.0%.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies focused on the specific detection of the top five STEC in cattle feces using PCR screening approaches. Nevertheless, the low number of cattle screened and the fact that serogroup-specific strain isolation was restricted to only a few samples did not allow to obtain a reliable estimate of the prevalence of the top five EHEC (14)(15)(16)(17)(18).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Bibbal

Loukiadis

Kérourédan

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

The main pathogenic enterohemorrhagic Escherichia coli (EHEC) strains are defined as Shiga toxin (Stx)-producing E. coli (STEC) belonging to one of the following serotypes: O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28. Each of these five serotypes is known to be associated with a specific subtype of the intimin-encoding gene (eae). The objective of this study was to evaluate the prevalence of bovine carriers of these "top five" STEC in the four adult cattle categories slaughtered in France. Fecal samples were collected from 1,318 cattle, including 291 young dairy bulls, 296 young beef bulls, 337 dairy cows, and 394 beef cows. A total of 96 E. coli isolates, including 33 top five STEC and 63 atypical enteropathogenic E. coli (aEPEC) isolates, with the same genetic characteristics as the top five STEC strains except that they lacked an stx gene, were recovered from these samples. O157:H7 was the most frequently isolated STEC serotype. The prevalence of top five STEC (all serotypes included) was 4.5% in young dairy bulls, 2.4% in young beef bulls, 1.8% in dairy cows, and 1.0% in beef cows. It was significantly higher in young dairy bulls (P < 0.05) than in the other 3 categories. The basis for these differences between categories remains to be elucidated. Moreover, simultaneous carriage of STEC O26:H11 and STEC O103:H2 was detected in one young dairy bull. Lastly, the prevalence of bovine carriers of the top five STEC, evaluated through a weighted arithmetic mean of the prevalence by categories, was estimated to 1.8% in slaughtered adult cattle in France.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Bibbal

Loukiadis

Kérourédan

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In one of the studies, which did not include IMS as part of the protocol, only EHEC O26 was detected, and this serogroup was found in only 2 (0.4%) of 450 carcasses and 0 of 450 hides (49). In the other study from Ireland, IMS was used for EHEC O26, O103, O111, and O145, and of 402 hides and carcasses cultured, 1 hide was positive for O26 (0.2%) but no other EHEC-6 serogroups were found (65). In one study that was conducted on cattle at an abattoir in Poland but did not specify whether the animals were of beef or dairy origin, 400 cm 2 of hide and carcass surface area were sampled, but the protocol did not include non-O157 IMS and no EHEC-6 isolates were obtained (74).…”

Section: Discussionmentioning

confidence: 95%

Prevalence and Level of Enterohemorrhagic Escherichia coli in Culled Dairy Cows at Harvest

Stromberg

Lewis

Aly

et al. 2016

Journal of Food Protection

View full text Add to dashboard Cite

The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) O26, O45, O103, O111, O121, and O145 (collectively EHEC-6) plus EHEC O157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n =300) were collected from 100 cows at harvest and tested by a culture-based method and two molecular methods: NeoSEEK STEC (NS) and Atlas STEC EG2 Combo. Both the culture and NS methods can be used to discriminate among the seven EHEC types (EHEC-7), from which the cumulative prevalence was inferred, whereas the Atlas method can discriminate only between EHEC O157 and non-O157 EHEC, without discrimination of the serogroup. The EHEC-7 prevalence in feces, hides, and carcass surfaces was 6.5, 15.6, and 1.0%, respectively, with the culture method and 25.9, 64.9, and 7.0%, respectively, with the NS method. With the Atlas method, the prevalence of non-O157 EHEC was 29.1, 38.3, and 28.0% and that of EHEC O157 was 29.1, 57.0, and 3.0% for feces, hides, and carcasses, respectively. Only two samples (a hide sample and a fecal sample) originating from different cows contained quantifiable EHEC. In both samples, the isolates were identified as EHEC O157, with 4.7 CFU/1,000 cm2 in the hide sample and 3.9 log CFU/g in the fecal sample. Moderate agreement was found between culture and NS results for detection of EHEC O26 (κ =0.58, P < 0.001), EHEC O121 (κ = 0.50, P < 0.001), and EHEC O157 (κ = 0.40, P < 0.001). No significant agreement was observed between NS and Atlas results or between culture and Atlas results. Detection of an EHEC serogroup in fecal samples was significantly associated with detection of the same EHEC serogroup in hide samples for EHEC O26 (P =0.001), EHEC O111 (P =0.002), EHEC O121 (P < 0.001), and EHEC-6 (P = 0.029) based on NS detection and for EHEC O121 (P < 0.001) based on detection by culture. This study provides evidence that non-O157 EHEC are ubiquitous on hides of culled dairy cattle and that feces are an important source of non-O157 EHEC hide contamination.

show abstract

“…En la década de los noventa, también se presentaron brotes en Canadá, Reino Unido, Bélgica, Irlanda, África y Nueva Zelanda (5)(6)(7)(8). Dos de los mayores brotes se han registrado en Japón e Inglaterra.…”

unclassified

Synthesis of antibiotic loaded polylactic acid nanoparticles and their antibacterial activity against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus

et al. 2017

View full text Add to dashboard Cite

Introducción. Las nanopartículas poliméricas constituyen una herramienta nanotecnológica que podría ayudar a combatir los microorganismos patógenos que han desarrollado resistencia a los antibióticos convencionales.Objetivo. Sintetizar nanopartículas de ácido poliláctico cargadas con ofloxacina y vancomicina, y determinar su actividad antibacteriana frente a Escherichia coli O157:H7 y Staphylococcus aureus resistente a la meticilina (SARM).Materiales y métodos. Las nanopartículas de ácido poliláctico cargadas con ofloxacina y vancomicina se sintetizaron utilizando el método de emulsión y evaporación de solvente. Se caracterizaron mediante dispersión de luz en modo dinámico, electroforesis Doppler con láser y microscopía electrónica de barrido (S-TEM). Se evaluó la actividad antibacteriana in vitro de las nanopartículas de ácido poliláctico con ofloxacina contra E. coli O157:H7 y nanopartículas de ácido poliláctico con vancomicina contra SARM, mediante el método de microdilución en caldo.Resultados. Se obtuvieron nanopartículas poliméricas con tamaños inferiores a 379 nm y carga superficial positiva de hasta 21 mV. Las nanopartículas cargadas con ofloxacina presentaron una concentración inhibitoria mínima (CIM50) de 0,001 μg/ml frente a E. coli O157:H7, valor 40 veces menor que la concentración de antibiótico libre necesaria para lograr el mismo efecto (CIM50=0,04 μg/ml). Para SARM, las nanopartículas mejoraron la potencia farmacológica in vitro de la vancomicina alexhibir una MIC50 de 0,005 μg/ml, comparada con la de 0,5 μg/ml del antibiótico libre.Conclusiones. Se mejoró el efecto antibacteriano de la ofloxacina y la vancomicina incorporadas en la matriz polimérica de ácido poliláctico. Las nanopartículas poliméricas constituirían una alternativa para el control de cepas bacterianas de interés en salud pública.

show abstract

Tracking verocytotoxigenic Escherichia coli O157, O26, O111, O103 and O145 in Irish cattle

Cited by 65 publications

References 46 publications

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Prevalence of Carriage of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among Slaughtered Adult Cattle in France

Prevalence and Level of Enterohemorrhagic Escherichia coli in Culled Dairy Cows at Harvest

Synthesis of antibiotic loaded polylactic acid nanoparticles and their antibacterial activity against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus

Contact Info

Product

Resources

About