Tracking transplanted cells in live animal using upconversion fluorescent nanoparticles

Multifunctional Mesoporous Nanoellipsoids for Biological Bimodal Imaging and Magnetically Targeted Delivery of Anticancer Drugs

“…These kinds of multifunctional mesoporous ellipsoidal nanocarriers could be potentially employed for simultaneous MRI/fl uorescence imaging and therapeutic applications. Moreover, this synthetic approach could be extended to fabricate various kinds of multifunctional mesoporous nanocarriers simply by changing the negatively charged CdTe QDs with other functional nanocrystals, such as biocompatible/functional Au nanocrystals with plasma resonance/hyperthermia abilities [ 43 ] and NaFY4:Er 3 + /Yb 3 + nanocrystals with upconversion fl uorescent properties, [ 44 ] or replacing positively charged polyelectrolytes with negatively charged ones (such as poly(sodium styrenesulfonate)) to satisfy various bio-application requirements. [ 22 ] Therefore this strategy provides a versatile route for incorporating diverse functionalities into single composite nanoparticles.…”

Harmonic Nanoparticles for Regenerative Research

“…The approach described in this protocol is limited to optical tracking and, although the possibility of using infrared excitation leads to increased penetration depth with respect to fluorescence based techniques; optical imaging cannot be extended to whole body and therefore should be complemented by magnetic detection 21 . Previous published work showed several approaches to assess structure and movement of cardiac cell clusters, including labeling by fluorescent dyes 22 , quantum dots 23,24 , super-paramagnetic iron oxide nanoparticles 25 , and up-conversion fluorescent particles [26][27][28] . The advantages of HNPs with respect to these nanoprobes are associated with absence of bleaching over extended periods of time, increased imaging penetration, wavelength tunability for increased contrast with respect to autofluorescence.…”

Up-Conversion Nanoparticles for Gastric Cancer Targeted Imaging and Therapy

“…Li's group reported a series of articles related the UCNPs for lymphatic imaging [63][64][65]. Niagara et al applied silica/NaYF 4 : Yb, Er to dynamically track live myoblast cells in vitro and in a living mouse model of cryo-injured hind limb [66]. In vivo confocal imaging of nanoparticle-loaded cells intravenously injected into a mouse tail vein showed them flowing in the ear blood vessels.…”