Tracking tick-borne diseases in Mongolian livestock using next generation sequencing (NGS)

Chaorattanakawee, Suwanna; Wofford, Rachel N.; Takhampunya, Ratree; Poole-Smith, B. Katherine; Boldbaatar, Bazartseren; Lkhagvatseren, Sukhbaatar; Altantogtokh, Doniddemberel; Musih, Elisha; Nymadawa, Pagbajabyn; Davidson, Silas A.; Hertz, Jeffrey C.; Fiorenzano, Jodi M.; Gray, Gregory C.; Fricken, Michael E. von

doi:10.1016/j.ttbdis.2021.101845

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…This also held true when examining previous exposure to Anaplasma spp., which was detected in 37% of nomadic herders and over 40% of livestock ( von Fricken et al, 2018 ). We also have detected Anaplasma ovis infection rates as high as 80% in sheep and 69% in goats, which aligns with what has previously been detected in ticks from the same region ( Ochirkhuu et al, 2017 ; von Fricken et al, 2018 , 2020a ; Enkhtaivan et al, 2019 ; Fischer et al, 2020 ; Chaorattanakawee et al, 2022 ). Anaplasmosis in livestock can result in anoxia, abortions, infertility, significant weight loss, and even death, all of which can impact economic security in pastoralist communities.…”

Section: Introductionsupporting

confidence: 90%

“…Human infections with this microbe have been reported, resulting in flu-like symptoms, bite-site lesion, neurological symptoms, and heart irregularities ( Maggi et al, 2009 ). Although our group has previously reported B. melophagi within Mongolian sheep, to our knowledge, this is the first report of B. melophagi within ticks collected from Mongolia ( Chaorattanakawee et al, 2022 ). Of note, this Bartonella species has been reported in Dermacentor, Hyalomma, and Rhipicephalus ticks collected from Xinjiang, China ( Ni et al, 2021 ).…”

Section: Discussionmentioning

confidence: 65%

“…Nested PCR was performed as described in Chaorattanakawee et al, 2022 to amplify both the V1-V6 region and V3-V4 region of the bacterial 16S rDNA. Each round of PCR included both an ultrapure DNA/RNA-free water negative control and a mock DNA extraction control.…”

Section: Methodsmentioning

confidence: 99%

“…Each round of PCR included both an ultrapure DNA/RNA-free water negative control and a mock DNA extraction control. The nested PCR amplicon products were isolated using AMPure XP magnetic beads and the quality of the products was assessed as previously described ( Chaorattanakawee et al, 2022 ). Amplicon products were stored at −20°C until further analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The variety of tick-borne pathogens found within Mongolia complicates attempts to fully characterize pathogens found in samples collected within the country. Our research group has recently used an analytical workflow on livestock blood samples from three aimags in Mongolia, initially applying next-generation sequencing (NGS) to obtain a snapshot of pathogen groups present, followed by conventional PCR and Sanger sequencing for confirmation and species characterization ( Chaorattanakawee et al, 2022 ). In this study, we deploy Next-Generation Sequencing for on Dermacentor ticks collected from a wide geographic range of Mongolia to further our understanding of tick-borne pathogens in this region.…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic profiles of Dermacentor tick pathogens from across Mongolia, using next generation sequencing

et al. 2022

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Tick-borne diseases are a major public health concern in Mongolia. Nomadic pastoralists, which make up ~ 26% of Mongolia’s population, are at an increased risk of both tick bite exposure and economic loss associated with clinical disease in herds. This study sought to further characterize tick-borne pathogens present in Dermacentor ticks (n = 1,773) sampled in 2019 from 15 of Mongolia’s 21 aimags (provinces). The ticks were morphologically identified and sorted into 377 pools which were then screened using Next-Generation Sequencing paired with confirmatory PCR and DNA sequence analysis. Rickettsia spp. were detected in 88.33% of pools, while Anaplasma spp. and Bartonella spp. were detected in 3.18 and 0.79% of pools, respectively. Khentii had the highest infection rate for Rickettsia spp. (76.61%; CI: 34.65–94.79%), while Arkhangai had the highest infection rate for Anaplasma spp. (7.79%; CI:4.04–13.72%). The exclusive detection of Anaplasma spp. in tick pools collected from livestock supports previous work in this area that suggests livestock play a significant role in disease maintenance. The detection of Anaplasma, Bartonella, and Rickettsia demonstrates a heightened risk for infection throughout Mongolia, with this study, to our knowledge, documenting the first detection of Bartonella melophagi in ticks collected in Mongolia. Further research deploying NGS methods is needed to characterize tick-borne pathogens in other endemic tick species found in Mongolia, including Hyalomma asiaticum and Ixodes persulcatus.

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