Tracking the Orchestration of the Tricarboxylic Acid Pathway in Plants, 80 Years After the Discovery of the Krebs Cycle

Tcherkez, Guillaume

doi:10.1007/978-3-319-68703-2_14

Cited by 6 publications

(6 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other species such as rapeseed ( Brassica napus ) contain citrate and have a very small amount of fumarate, suggesting that PEPC activity in the light might be lower. Nutrient availability (N, P and K) is also likely to reorchestrate respiratory metabolism (Rivas‐Ubach et al ., ) and change PEPC activity accordingly, but this has never been examined quantitatively (for a review, see Tcherkez, ). The effect of light would also be of great interest, particularly at low light (i.e.…”

Section: Discussionmentioning

confidence: 99%

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates

Abadie

Tcherkez

2018

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary Phosphenolpyruvate carboxylase (PEPC)‐catalysed fixation of bicarbonate to C4 acids is commonly believed to represent a rather small flux in illuminated leaves. In addition, its potential variation with O2 and CO2 is not documented and thus is usually neglected in gas‐exchange studies. Here, we used quantitative NMR analysis of sunflower leaves labelled with 13CO2 (99% 13C) under controlled conditions and measured the amount of 13C found in the four C‐atom positions in malate, the major product of PEPC activity. We found that amongst malate 13C‐isotopomers present after labelling, most molecules were labelled at both C‐1 and C‐4, showing the incorporation of 13C at C‐4 by PEPC fixation and subsequent redistribution to C‐1 by fumarase (malate–fumarate equilibrium). In addition, absolute quantification of 13C content showed that PEPC fixation increased at low CO2 or high O2, and represented up to 1.8 μmol m−2 s−1, that is, 40% of net assimilation measured by gas exchange under high O2/CO2 conditions. Our results show that PEPC fixation represents a quantitatively important CO2‐fixing activity that varies with O2 and/or CO2 mole fraction and this challenges the common interpretation of net assimilation in C3 plants, where PEPC activity is often disregarded or considered to be constant at a very low rate.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates

Abadie

Tcherkez

2018

New Phytologist

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to temperature, nutrient availability is also a fundamental parameter that dictates respiration rate, and this not only includes N but also phosphorus (P), sulphur (S) or potassium (Tcherkez, 2017). Using a survey across many species of different life forms grown under contrasted N/P conditions, Crous et al (2017) demonstrated the relationship between R light (measured with the Kok method and expressed per leaf surface area) and N and P elemental content (per leaf surface area).…”

Section: How Does Day Respiration Respond To Environmental Constraints?mentioning

confidence: 99%

Section: How Does Day Respiration Respond To Environmental Constraints?mentioning

confidence: 99%

Unravelling mechanisms and impacts of day respiration in plant leaves: an introduction to a Virtual Issue

Tcherkez

Atkin

2021

New Phytologist

Self Cite

View full text Add to dashboard Cite

Unravelling mechanisms and impacts of day respiration in plant leaves: an introduction to a Virtual Issue Leaf respiration plays a key role in plant primary production due to its roles in determining rates of daily net carbon gain, nutrient acquisition, and growth. It is now well-accepted that leaf respiration differs considerably between darkness and light, not only in rate but also in its metabolic pathways and biological functions (Tcherkez et al., 2012). As such, it is common practice to use different terms: light (day) and dark (night) respiration, with associated variables describing their rates, R light (R d) and R dark (R n). While leaf dark respiration has been documented for a long time, including its well-known relationship with growth, substrate supply and energy demand (O'Leary et al., 2019), there is still considerable uncertainty about many aspects of day respiration including: (1) how to measure the rate R light ; (2) metabolic mechanisms underlying carbon dioxide (CO 2) release and oxygen (O 2) consumption; and (3) its impact on photosynthesis. This persistent uncertainty is highly problematic because it affects calculations of photosynthetic parameters, our understanding of nitrogen (N) assimilation and how it interacts with photorespiration, and calculations of carbon use efficiency or gross primary production at the canopy and/or ecosystem scale. This Virtual Issue compiles 33 articles that provide significant advances in addressing these challenges, propose new techniques to measure R light , quantitatively assess the impact of R light on calculated rates of carbon exchange in leaves, or enhance our understanding about day respiration through the documentation of diel patterns in R dark. A specific review on day respiration can be found in Tcherkez et al. (2017b). Key aspects related to day respiration that are discussed in this Editorial are summarized in Fig. 1.

show abstract

“…In phenomenological terms, respiratory temperature responses are readily captured via three parameters in an extended Arrhenius-type model (Equation 4). Nonetheless, the above scenarios illustrate complex underlying physiology (i.e., Tcherkez, 2017). In particular, assumption of entirely temperature-invariant UQ red /UQ ox in vivo might be an oversimplification (Kruse et al, 2011).…”

Section: Temperature-dependent Changes In Activation Energy and Undermentioning

confidence: 99%

Plasticity of Leaf Respiratory and Photosynthetic Traits in Eucalyptus grandis and E. regnans Grown Under Variable Light and Nitrogen Availability

Kruse

Turnbull

Rennenberg³

et al. 2020

Front. For. Glob. Change

View full text Add to dashboard Cite

Tracking the Orchestration of the Tricarboxylic Acid Pathway in Plants, 80 Years After the Discovery of the Krebs Cycle

Cited by 6 publications

References 58 publications

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates

Unravelling mechanisms and impacts of day respiration in plant leaves: an introduction to a Virtual Issue

Plasticity of Leaf Respiratory and Photosynthetic Traits in Eucalyptus grandis and E. regnans Grown Under Variable Light and Nitrogen Availability

Contact Info

Product

Resources

About

Tracking the Orchestration of the Tricarboxylic Acid Pathway in Plants, 80 Years After the Discovery of the Krebs Cycle

Cited by 6 publications

References 58 publications

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO2 and O2 mole fractions and represents a major flux at high photorespiration rates

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO2 and O2 mole fractions and represents a major flux at high photorespiration rates

Unravelling mechanisms and impacts of day respiration in plant leaves: an introduction to a Virtual Issue

Plasticity of Leaf Respiratory and Photosynthetic Traits in Eucalyptus grandis and E. regnans Grown Under Variable Light and Nitrogen Availability

Contact Info

Product

Resources

About

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates

In vivo phosphoenolpyruvate carboxylase activity is controlled by CO₂ and O₂ mole fractions and represents a major flux at high photorespiration rates