Tracking the gastrointestinal digestive and metabolic behaviour of <i>Dendrobium officinale</i> polysaccharides by fluorescent labelling

Wu, Zhijing; Zhang, Yu; Nie, Gang; Liu, Junxi; Mei, Hao; He, Zihao; Dou, Pengfei; Wang, Kaiping

doi:10.1039/d2fo01506d

Cited by 13 publications

(2 citation statements)

References 48 publications

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“…Modern pharmacological studies have reported that the polysaccharides of P. cyrtonema have various pharmacological activities such as hypoglycemic [4], hypolipidemic [5], anti-in ammatory [6], immune-regulating [7], antioxidant, and antiaging effects [8]. The complex structure of polysaccharides, as well as the lack of easily detectable ultraviolet (UV) absorbing groups and chromophores, has made quantitative analysis di cult [9,10]. Therefore, studies on the pharmacokinetics, tissue distribution, and mechanisms of action of polysaccharides are limited.…”

mentioning

confidence: 99%

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

Yong,

Zhang,

Cao

et al. 2024

Preprint

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Polygonatum cyrtonema is a medicinal plant and its polysaccharides are used for immunomodulation and the treatment of hypoglycemia. Investigation of the tissue distribution and pharmacokinetics of P. cyrtonema polysaccharide can further elucidate its pharmacological mechanism. A fluorescence labeling approach using rhodamine B (RhB) as a fluorescent molecular probe was used for the quantitative assessment of the polysaccharide from dried P. cyrtonema (DPC1) samples, and the pharmacokinetics and tissue distribution of DPC1 were evaluated in mice after intraperitoneal or oral administration. DPC1 was successfully labeled with RhB, showing degrees of fluorescence labeling at 0.453% and 0.568% as determined by the ultraviolet and enzyme marker methods, respectively. DPC1-RhB was rapidly absorbed into the bloodstream after oral and intraperitoneal administration. The relative bioavailability of DPC1-RhB was as high as 48.648%, showing linear pharmacokinetic characteristics. After administration, DPC1-RhB was primarily distributed in the tissues of the heart, spleen, and lung, indicating that the drug has a targeted effect on these tissues. Overall, the findings provide a comprehensive reference for the in vivo distribution of DPC1, together with a foundation for further elucidation of its pharmacological mechanism and the development and application of DPC1 formulations.

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mentioning

confidence: 99%

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

Yong,

Zhang,

Cao

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Modern pharmacological studies have reported that the polysaccharides of P. cyrtonema have various pharmacological activities such as hypoglycemic [ 6 ], hypolipidemic [ 7 ], anti-inflammatory [ 8 ], immune-regulating [ 9 ], antioxidant, and antiaging effects [ 10 ]. The complex structure of polysaccharides, as well as the lack of easily detectable ultraviolet (UV) absorbing groups and chromophores, has made quantitative analysis difficult [ 11 , 12 ]. Therefore, studies on the pharmacokinetics, tissue distribution, and mechanisms of action of polysaccharides are limited.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Pharmacokinetic Study of Polygonatum cyrtonema Polysaccharide DPC1 after Oral and Intraperitoneal Administration

Yong,

Zhang,

Cao

et al. 2024

Pharmaceuticals

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(1) Background: Polygonatum cyrtonema is a medicinal plant, and its polysaccharides are used for immunomodulation and the treatment of hyperglycemia. Investigation of the tissue distribution and pharmacokinetics of P. cyrtonema polysaccharide can further elucidate its pharmacological mechanisms. (2) Methods: A fluorescence-labeling approach using rhodamine B (RhB) as a fluorescent molecular probe was used for the quantitative assessment of the polysaccharide from dried P. cyrtonema (DPC1) samples, and the pharmacokinetics and tissue distribution of DPC1 were evaluated in mice after intraperitoneal or oral administration. (3) Results: DPC1 was successfully labeled with RhB, showing degrees of fluorescence labeling at 0.453% and 0.568% as determined by the ultraviolet and enzyme marker methods, respectively. DPC1-RhB was rapidly absorbed into the bloodstream after oral and intraperitoneal administration. Pharmacokinetic characteristics showed that oral administration and intraperitoneal administration were consistent with the features of a two-compartment model. (4) Conclusion: After administration, DPC1-RhB was primarily distributed in the tissues of the heart, spleen, and lung, indicating that the drug has a targeted effect on these tissues. Overall, the findings provide a comprehensive reference for the in vivo distribution of DPC1, together with a foundation for further elucidation of its pharmacological mechanisms and the development and application of DPC1 formulations.

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Degradation of Angelica sinensis polysaccharide: Structures and protective activities against ethanol-induced acute liver injury

Zhang,

Wang,

Zheng

et al. 2024

Carbohydrate Polymers

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Tracking the gastrointestinal digestive and metabolic behaviour of Dendrobium officinale polysaccharides by fluorescent labelling

Abstract: Recently, Dendrobium officinale polysaccharide (DOP), a typical acetylated glucomannan, has been widely applied in functional foods owing to its excellent bioactivity. However, the insufficiency of studies on in vivo process...

Cited by 13 publications

References 48 publications

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

In Vivo Pharmacokinetic Study of Polygonatum cyrtonema Polysaccharide DPC1 after Oral and Intraperitoneal Administration

Degradation of Angelica sinensis polysaccharide: Structures and protective activities against ethanol-induced acute liver injury

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