Tracking Stem Cell Differentiation in the Setting of Automated Optogenetic Stimulation

Stroh, Albrecht; Tsai, Hsing-Chen; Wang, Li Ping; Zhang, Feng; Kressel, Jenny; Aravanis, Alex; Santhanam, Nandhini; Deisseroth, Karl; Konnerth, Arthur; Schneider, M. Bret

doi:10.1002/stem.558

Cited by 85 publications

(66 citation statements)

References 60 publications

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“…Oxidative stress induces cell-membrane depolarization by disturbing its assembly, which in turn causes changes in membrane fluidity and permeability, alterations of ion transport, and the inhibition of metabolic processes. 64 Depolarization of the cell membrane modulates differentiation processes in dividing neural cells, 65,66 and the regulation of ROS level seems to be critical for neuronal development. 67 As the control of stem cell migration and differentiation is a major concern in stem cell-based therapy, it would be advisable that NP applications for stem cell labeling are free of any unexpected perturbation in CMP and ROS production.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Pongrac¹,

Pavičić²,

Milić³

et al. 2016

IJN

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Biocompatibility, safety, and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in researching their application in biomedicine. One improvement in the biological properties of SPIONs may be achieved by different functionalization and surface modifications. This study aims to investigate how a different surface functionalization of SPIONs – uncoated, coated with d -mannose, or coated with poly- l -lysine – affects biocompatibility. We sought to investigate murine neural stem cells (NSCs) as important model system for regenerative medicine. To reveal the possible mechanism of toxicity of SPIONs on NSCs, levels of reactive oxygen species, intracellular glutathione, mitochondrial membrane potential, cell-membrane potential, DNA damage, and activities of SOD and GPx were examined. Even in cases where reactive oxygen species levels were significantly lowered in NSCs exposed to SPIONs, we found depleted intracellular glutathione levels, altered activities of SOD and GPx, hyperpolarization of the mitochondrial membrane, dissipated cell-membrane potential, and increased DNA damage, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly, indicating that mitochondrial homeostasis is their major cellular target. Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Pongrac¹,

Pavičić²,

Milić³

et al. 2016

IJN

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“…Optogenetics was used to validate blood oxygen level-dependent MRI signals via direct links to local electric events (79). More translational studies tackled questions related to epilepsy and termination of seizures (123), Parkinson's disease, and deep brain stimulation to counteract it (7,44,77), countering visual degeneration in retinitis pigmentosa (21), resuming respiratory control (43), better optical nerve stimulation of skeletal muscle (83), and optimized stem cell differentiation (119,131). This is a small subset of the wide spectrum of studies conducted thus far; recent more comprehensive reviews provide further information (41,136).…”

Section: Optogenetics In Neurosciencementioning

confidence: 99%

Cardiac optogenetics

Entcheva

2013

American Journal of Physiology-Heart and Circulatory Physiology

113

105

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Optogenetics is an emerging technology for optical interrogation and control of biological function with high specificity and high spatiotemporal resolution. Mammalian cells and tissues can be sensitized to respond to light by a relatively simple and well-tolerated genetic modification using microbial opsins (light-gated ion channels and pumps). These can achieve fast and specific excitatory or inhibitory response, offering distinct advantages over traditional pharmacological or electrical means of perturbation. Since the first demonstrations of utility in mammalian cells (neurons) in 2005, optogenetics has spurred immense research activity and has inspired numerous applications for dissection of neural circuitry and understanding of brain function in health and disease, applications ranging from in vitro to work in behaving animals. Only recently (since 2010), the field has extended to cardiac applications with less than a dozen publications to date. In consideration of the early phase of work on cardiac optogenetics and the impact of the technique in understanding another excitable tissue, the brain, this review is largely a perspective of possibilities in the heart. It covers the basic principles of operation of light-sensitive ion channels and pumps, the available tools and ongoing efforts in optimizing them, overview of neuroscience use, as well as cardiac-specific questions of implementation and ideas for best use of this emerging technology in the heart. channelrhodopsin; light-sensitive ion channels; optical mapping THIS ARTICLE is part of a collection on Physiological Basis of Cardiovascular Cell and Gene Therapies. Other articles appearing in this collection, as well as a full archive of all collections, can be found online at http://ajpheart.physiology.org/. Background

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“…Из них 24, выполнен-ных на МСККМ [12,; 2 -на цельном кост-ном мозге (КМ) [53,54]; 9 -на МСКЖТ [17,31,[55][56][57][58][59][60][61]; 5 -на гематопоэтических СК (ГСК) [62][63][64][65][66]; 4 -на СК нервной ткани (НСК) [67][68][69][70]; 2 -на ЭСК [71,72]; 7 -на СК пульпы зуба (СКпЗ) [73][74][75][76][77][78] и перидонта [79]; 4 -на МСК пупочного канатика, печени, сердца и эпидермиса [49,[80][81][82] соответственно] и 2 -на необластах планарий Dugesia tigrina [83,84]. В анализ были включены 6 ра-бот, выполненных на фетальных (кальвариальных) клетках свода черепа крысы (ФКК) [85][86][87][88][89][90], счита-ющихся недифференцированными СК [91]; 8 -на сателлитных клетках поперечнополосатых мышц (СатК) [92][93][94]…”

Section: материал и методыunclassified

The application of stem cells, visible and infrared light in regenerative medicine. Part 1

Emel'yanov

Kiryanova

2015

Vopr. kurortol. fizioter. lech. fiz. kul't.

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51Основной задачей регенеративной медицины (РМ) является восстановление утраченных в резуль-тате тех или иных болезней или повреждений струк-туры и функции органов и тканей. человеческий организм может регенерировать на уровне клеточ-ного пласта, состоящего из одного или, может быть, нескольких видов близкородственных клеток. Дан-ное свойство используется в медицине для восста-новления кожного эпителия, при переломах костей, разрывах сухожилий, мышц, повреждении нервных стволов и пр. Однако после повреждения несколь-ких слоев тканей (например, кожного эпителия и подлежащей дермы) или нарушения структуры тка-ней и органов всегда остаются рубцы, что является наглядным свидетельством неполной регенерации. при подобных повреждениях для полного восста- Health, ul. kirochnaya, 41, Sankt-Peterburg, Russia, 191015 Несмотря на длительную историю фотохромотерапии, до сих пор не существует достаточной теоретической базы для вы-бора таких параметров облучения, как плотность мощности, доза облучения и время экспозиции. Также не существует обобщений применения света в области регенеративной медицины. Цель обзора -обобщить данные экспериментальных исследований, связанных с воздействием света видимого и инфракрасного диапазонов на стволовые клетки (Ск) и как следствие уточнить рекомендации по выбору параметров облучения и углубить их научное обоснование. Результаты. Проанализированы 78 оригинальных статей, в которых представлены результаты исследований, выполненных на различ-ных типах Ск. их качество, количество и структура свидетельствуют о начальном (инициальном) этапе развития данной области медицины. Анализ выявил значимость типа Ск при выборе параметров облучения. Наибольших прибавок скоро-стей пролиферации и дифференцировки авторы добивались при высоких плотностях мощности (более 50 мвт/см 2 ) и низ-ких дозах (около 1 Дж/см 2 ) и времени экспозиции (секунды). Заключение. общие выводы для 1-й и 2-й частей данного обзора будут представлены в следующем номере данного журнала. Ключевые слова: фотохромотерапия, свет, стволовые клетки, фототерапия, регенеративная медицина.Objective: the present article was designed to overview the experimental studies of visible and infrared light irradiation of human and animal stem cells (SC) in vitro and in vivo for the evaluation of its photobiomodulatory effects. The results will be used to elaborate substantiation for the choice of the parameters of SC light irradiation and to develop recommendations for the application of this method in regenerative medicine (Rm). Background: the clinical application of light irradiation is a matter of contrsy, in the first place due to the difficulties encountered in the rational choice of irradiation parameters. The theoretical substantiation of such choice remains a stumbling block too despite the long history of photoghromotherapy. There is thus far no reliable theoretical basis for the adequate choice of such irradiation parameters as power density, radiation dose, and exposure time. The experiences with the light application for the purpose of ...

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Tracking Stem Cell Differentiation in the Setting of Automated Optogenetic Stimulation

Cited by 85 publications

References 60 publications

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Cardiac optogenetics

The application of stem cells, visible and infrared light in regenerative medicine. Part 1

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