Tracking spatio-temporal distribution and transmission of antibiotic resistance in aquatic environments by using ESBL-producing Escherichia coli as an indicator

Li, Qi; Zou, Huiyun; Wang, Di; Zhao, Ling; Meng, Min; Wang, Zhongyi; Wu, Tianle; Wang, Shuang; Li, Xuewen

doi:10.1016/j.jenvman.2023.118534

Cited by 3 publications

(3 citation statements)

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“…Notably, discharge from bovine and poultry slaughterhouses and farms can introduce ESBL-producing microorganisms into aquatic ecosystems, facilitating their dissemination among animal and human populations [ 32 , 33 , 34 ]. Moreover, improper effluent disposal, particularly when contaminated with human feces, leads to high levels of aquatic contamination by ESBL-producing E. coli , underscoring the urgency of preventing the spread of these isolates to human and animal populations [ 35 ]. Of particular concern are wastewater discharges from hospitals and healthcare facilities, which have been strongly implicated in the dissemination of ESBL-producing enterobacteria [ 36 , 37 ] ( Figure 1 ).…”

Section: Esbl-producing E Coli In Farm Animalsmentioning

confidence: 99%

Exploring Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Food-Producing Animals and Animal-Derived Foods

Ribeiro,

Nespolo,

Rossi

et al. 2024

Pathogens

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Antimicrobials serve as crucial treatments in both veterinary and human medicine, aiding in the control and prevention of infectious diseases. However, their misuse or overuse has led to the emergence of antimicrobial resistance, posing a significant threat to public health. This review focuses on extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in animals and their associated food products, which contribute to the proliferation of antimicrobial-resistant strains. Recent research has highlighted the presence of ESBL-producing E. coli in animals and animal-derived foods, with some studies indicating genetic similarities between these isolates and those found in human infections. This underscores the urgent need to address antimicrobial resistance as a pressing public health issue. More comprehensive studies are required to understand the evolving landscape of ESBLs and to develop strategic public health policies grounded in the One Health approach, aiming to control and mitigate their prevalence effectively.

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Section: Esbl-producing E Coli In Farm Animalsmentioning

confidence: 99%

Exploring Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Food-Producing Animals and Animal-Derived Foods

Ribeiro,

Nespolo,

Rossi

et al. 2024

Pathogens

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“…e circulation of Escherichia coli ESBL is very widespread, including from domestic animals (Falodun et al, 2022), wildlife (Dalazen et al, 2023), agricultural-livestock environments (Al-Mustapha et al, 2023), and healthcare settings (Azuma et al, 2022), thus having the potential to spread resistance. Recent evidence shows that the large proportion of Escherichia coli ESBL in the aquatic environment is of great concern, and these bacteria are found in human fecal samples, which contributes to the presence of antibiotic resistance in the aquatic environment (Li et al, 2023). e majority of plasmid-encoded ESBLs are of the TEM, SHV, or CTX-M types, with CTX-M being the most common ESBL type in Escherichia coli and K. pneumonia (Rossolini et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL

Wiradana,

Permatasari,

Sari

et al. 2024

J. Appl. Vet. Sci. Technol

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Background: The marine environment is the main source of research on natural products in the future. In addition, marine microorganisms have been identified as a natural source capable of developing new antibiotic compounds, including controlling urinary tract infections caused by Escherichia coli ESBL. Purpose: This study aims to isolate, select, and test the potential of brown macroalgae endophytic fungi (Phaeophyceae) collected from the coastal waters of Sanur, Bali Province. Methods: Three types of brown algae were collected from Sanur Beach and their endophytic fungi were isolated using PDA media. Antibacterial activity was determined by measuring the inhibition zone and determining the inhibition category. The selected isolates were cultured and the phytochemical profile was determined qualitatively. In addition, molecular identification using the Internal Transcribed Spacer (ITS) primer set and comparison with the GenBank (NCBI) database were carried out in this study. Results: The results showed that 10 isolates were successfully isolated from three types of brown macroalgae. It was found that isolates T1, S1, and P3 had the highest inhibition in the very strong category. There were variations in mycelial weight, pH value, and inhibition shown by the culture filtrate of the three endophytic fungal isolates against Escherichia coli ESBL. Isolate S1 had the highest phytochemical profile, namely alkaloids, triterpenoids, saponins, and phenolics. The three endophytic fungal isolates showed isolate T1 (Phlebiopsis magnicystidiata) (MT5617191), isolate P3 (Neurospora crassa strain RT3M) (MT1028551), and isolate S1 (Peniophora sp.) (MH2680421). Conclusion: The results of this study provide initial information regarding the potential of bioprospecting brown macroalgal endophytic fungi as a source of new antibiotics against Escherichia coli ESBL.

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“…Measurement of antibiotic-resistant E. coli concentrations has been proposed to study resistance in surface waters due to its extensive characterization as a model organism, ubiquity in the environment, and relevance in food safety (Berendonk et al, 2015;Anjum et al, 2021;WHO, 2021;Liguori et al, 2022;Bengtsson-Palme et al, 2023;Li et al, 2023). Antibiotic resistance is also frequently assessed for specific pathogenic microorganisms .…”

Section: Introductionmentioning

confidence: 99%

On composite sampling for monitoring generic and antibiotic-resistant coliforms in irrigation ponds

Stocker,

Smith,

Pachepsky

2024

Front. Water

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The presence of fecal bacteria in irrigation waters is well documented in causing human and animal illnesses, with the potential for antibiotic-resistant pathogens to increase the seriousness of these infections. Approaches to sampling fecal and antibiotic-resistant bacteria (ARB) in irrigation waters used in raw food production require standardization to quantify and discern potential spatiotemporal trends in antibiotic-resistant bacteria. Composite sampling is widely used to reduce the cost and time of processing samples while estimating spatial or temporal variation in contaminant concentrations. The objectives of this work were to evaluate the spatial variation in generic and ARB in several irrigation ponds and assess the effectiveness of composite sampling in estimating the average of individual samples. In a grid-like fashion, five irrigation ponds were sampled for generic and antibiotic-resistant E. coli and total coliforms using the Colilert Quanti-Tray/2000 system with and without tetracycline and cefotaxime added. Individual samples were composited in sample sets including all samples, only bank samples, and only interior samples. Coefficients of variations in general were high (> 100%) for generic bacteria and higher for ARB (140%−290%). Concentrations of all measured bacteria were lower in the pond interior locations than the banks. The percentage of tetracycline-resistant E. coli varied among ponds from averages of 0% to 23%. No cefotaxime-resistant E. coli were detected in any of the ponds whereas cefotaxime-resistant total coliforms were detected at each site. The average percentage of cefotaxime-resistant total coliforms varied from 1.1 to 13.8% among ponds. E. coli concentrations in composite samples did not significantly differ from either the mean or median of the individual sample sets in 89% and 83% of cases, respectively, indicating composite sampling to be effective in capturing spatial variation of both generic and ARB. Results of this work can be used to aid in the development of better strategies for surveilling antibiotic resistance in aquatic environments.

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Tracking spatio-temporal distribution and transmission of antibiotic resistance in aquatic environments by using ESBL-producing Escherichia coli as an indicator

Cited by 3 publications

References 44 publications

Exploring Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Food-Producing Animals and Animal-Derived Foods

Exploring Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Food-Producing Animals and Animal-Derived Foods

Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL

On composite sampling for monitoring generic and antibiotic-resistant coliforms in irrigation ponds

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