Tracking Se Assimilation and Speciation through the Rice Plant – Nutrient Competition, Toxicity and Distribution

Nothstein, Alexandra Kelly; Eiche, Elisabeth; Riemann, Michael; Nick, Peter; Winkel, Lenny H. E.; Göttlicher, Jörg; Steininger, Ralph; Brendel, Rita; Brasch, Matthias von; Konrad, Gabriele; Neumann, Thomas

doi:10.1371/journal.pone.0152081

Cited by 38 publications

(34 citation statements)

References 29 publications

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“…An increase in Se concentration in the shoots and grains of rice plants subjected to the application of this element was demonstrated by Hu et al (2002), Boldrin et al (2013), Hu et al (2014) and Nothstein et al (2016). This increase is related to the Se concentration mainly in the soil.…”

Section: Discussionmentioning

confidence: 91%

Selenium protects rice plants from water deficit stress

Andrade

Silva

Guimarães

et al. 2018

Ecotoxicology and Environmental Safety

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Selenium (Se) is essential to humans and animals due to its antioxidant properties. Although it is not considered an essential nutrient for higher plants. Many studies show that Se in low concentrations (up to 0.5 mg kg) provides beneficial effects to non-hyperaccumulating plants by participating in antioxidant defense systems and enhancing tolerance to abiotic stress. Therefore, this study aimed to evaluate the effects of Se application rates on rice plants under different soil water conditions. The experiment was conducted on an Oxisol using four Se rates (0, 0.5, 1.0 and 2.0 mg kg) and two soil water conditions (irrigated and water deficit). Selenium application via soil up to 0.5 mg kg increased the plant height, chlorophyll index, sulfur and copper accumulation in shoots, carbon dioxide assimilation, superoxide dismutase (EC 1.15.1.1) activity and decreased the hydrogen peroxide concentration in rice leaves. The accumulation of Se in shoot biomass and Se concentration in seeds increased linearly with the applied rates. Water deficit strongly decreased the plant growth and yield. However, rice plants treated with Se showed higher net photosynthesis, water use efficiency and antioxidant system. This study provides useful information about the roles of Se in protecting rice plants from water deficit stress.

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Section: Discussionmentioning

confidence: 91%

Selenium protects rice plants from water deficit stress

Andrade

Silva

Guimarães

et al. 2018

Ecotoxicology and Environmental Safety

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“…Se content in food is determined by the occurrence and bioavailability of this element in the soil and by the efficiency of the soileplant transfer system, both of which are highly variable worldwide. Thus, the Se nutritional status of human populations is usually geographically correlated, and agronomic biofortification has been proposed for various crops and regions to alleviate Se deficiency in humans (Winkel et al, 2015;Nothstein et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Natural variation of selenium in Brazil nuts and soils from the Amazon region

Wadt²,

et al. 2017

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Brazil nut tree (Bertholletia excelsa) is native of the Amazon rainforest. Brazil nuts are consumed worldwide and are known as the richest food source of selenium (Se). Yet, the reasoning for such Se contents is not well stablished. We evaluated the variation in Se concentration of Brazil nuts from Brazilian Amazon basin, as well as soil properties, including total Se concentration, of the soils sampled directly underneath the trees crown, aiming to investigate which soil properties influence Se accumulation in the nuts. The median Se concentration in Brazil nuts varied from 2.07 mg kg (in Mato Grosso state) to 68.15 mg kg (in Amazonas state). Therefore, depending on its origin, a single Brazil nut could provide from 11% (in the Mato Grosso state) up to 288% (in the Amazonas state) of the daily Se requirement for an adult man (70 μg). The total Se concentration in the soil also varied considerably, ranging from <65.76 to 625.91 μg kg, with highest Se concentrations being observed in soil samples from the state of Amazonas. Se accumulation in Brazil nuts generally increased in soils with higher total Se content, but decreased under acidic conditions in the soil. This indicates that, besides total soil Se concentration, soil acidity plays a major role in Se uptake by Brazil nut trees, possibly due to the importance of this soil property to Se retention in the soil.

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“…The minimum parameter plant cultivation is a modified approach after [ 28 ]. Sterilized nutrient-free phytoagar (Duchefa powder, 0.4 wt.%) was poured into sterilized Magenta boxes and doped homogeneously with sodium selenate and sodium selenite in concentrations of 500 and 1000 μg L -1 respectively.…”

Section: Methodsmentioning

confidence: 99%

Preparation and purification of organic samples for selenium isotope studies

et al. 2018

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Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms. As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere. Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone. Plant cultivation setups enable systematic controlled investigations, but samples derived from them–plant tissue and phytoagar–are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS. In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated. A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum. Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices. All these methods efficiently remove critical matrix elements, but differ in Se recovery and organic residues. Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements. Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰. For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰. The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.

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Tracking Se Assimilation and Speciation through the Rice Plant – Nutrient Competition, Toxicity and Distribution

Cited by 38 publications

References 29 publications

Selenium protects rice plants from water deficit stress

Selenium protects rice plants from water deficit stress

Natural variation of selenium in Brazil nuts and soils from the Amazon region

Preparation and purification of organic samples for selenium isotope studies

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