Tracking SARS-CoV-2 in rivers as a tool for epidemiological surveillance

Maidana-Kulesza, María Noel; Poma, Hugo Ramiro; Sanguino-Jorquera, Diego Gastón; Reyes, Sarita Isabel; Said-Adamo, María del Milagro; Mainardi-Remis, Juan Martín; Gutiérrez-Cacciabue, Dolores; Cristóbal, Héctor Antonio; Cruz, Mercedes Cecilia; González, María Belén Hernández; Rajal, Verónica Beatriz

doi:10.1016/j.scitotenv.2022.157707

Cited by 10 publications

(4 citation statements)

References 83 publications

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“…Surface waters heavily contaminated by sewage may also exhibit an epidemiological indicator function in terms of wastewater surveillance (e.g. (Kolarević et al 2022 , Maidana-Kulesza et al 2022 ).…”

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

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The impacts on faecal pollution analysis using nucleic acid-based methods, such as PCR and sequencing, in health-related water quality research were assessed by rigorous literature analysis. A wide range of application areas and study designs has been identified since the first application more than 30 years ago (>1,100 publications). Given the consistency of methods and assessment types, we suggest defining this emerging part of science as a new discipline: genetic faecal pollution diagnostics (GFPD) in health-related microbial water quality analysis. Undoubtedly, GFPD has already revolutionised faecal pollution detection and microbial source tracking, the current core applications. GFPD is also expanding to many other research areas, including infection and health risk assessment, evaluation of microbial water treatment, and support of wastewater surveillance. In addition, storage of DNA extracts allows for biobanking, which opens up new perspectives. The tools of GFPD can be combined with cultivation-based standardised faecal indicator enumeration, pathogen detection, and various environmental data types, in an integrated data analysis approach. This comprehensive meta-analysis provides the scientific status quo of this field, including trend analyses and literature statistics, outlining identified application areas, and discussing the benefits and challenges of nucleic acid-based analysis in GFPD.

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Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

View full text Add to dashboard Cite

show abstract

“…The present work is part of a federal research program aimed at examining wastewater to evaluate the circulation of the SARS-CoV-2 virus in Argentina during the pandemic. The investigations provided epidemiological data concerning different provinces; Buenos Aires [41,52], Mendoza [47], Santa Fé [53], Córdoba [54], and Salta [55,56]. It is the first and so far the only surveillance program carried out in the Province of Tucumán to develop a strategy for detecting SARS-CoV-2 in urban wastewater.…”

Section: Discussionmentioning

confidence: 99%

Wastewater monitoring of SARS‐CoV‐2 gene for COVID‐19 epidemiological surveillance in Tucumán, Argentina

D'Arpino,

Sineli,

Goroso

et al. 2024

J Basic Microbiol

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Wastewater‐based epidemiology provides temporal and spatial information about the health status of a population. The objective of this study was to analyze and report the epidemiological dynamics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in the province of Tucumán, Argentina during the second and third waves of coronavirus disease 2019 (COVID‐19) between April 2021 and March 2022. The study aimed to quantify SARS‐CoV‐2 RNA in wastewater, correlating it with clinically reported COVID‐19 cases. Wastewater samples (n = 72) were collected from 16 sampling points located in three cities of Tucumán (San Miguel de Tucumán, Yerba Buena y Banda del Río Salí). Detection of viral nucleocapsid markers (N1 gene) was carried out using one‐step reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Viral loads were determined for each positive sample using a standard curve. A positive correlation (p < 0.05) was observed between viral load (copies/mL) and the clinically confirmed COVID‐19 cases reported at specific sampling points in San Miguel de Tucumán (SP4, SP7, and SP8) in both months, May and June. Indeed, the high viral load concurred with the peaks of COVID‐19 cases. This method allowed us to follow the behavior of SARS‐CoV‐2 infection during epidemic outbreaks. Thus, wastewater monitoring is a valuable epidemiological indicator that enables the anticipation of increases in COVID‐19 cases and tracking the progress of the pandemic. SARS‐CoV‐2 genome‐based surveillance should be implemented as a routine practice to prepare for any future surge in infections.

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“…The present article is part of a federal research program aimed at examining wastewater to evaluate the circulation of the SARS-CoV-2 virus in Argentina during the pandemic. The investigations provided epidemiological data concerning different provinces; Buenos Aires (Barrios et al, 2021; Iglesias et al, 2021), Santa Fé (Reno et al, 2022), Mendoza (Giraud-Billoud, et al, 2021), Córdoba (Masachessi et al, 2022), Salta (Cruz et al, 2023; Maidana-Kulesza, et al, 2022). It is the first surveillance report carried out in the Province of Tucumán to develop a strategy for detecting SARS-CoV-2 at the city level from wastewater.…”

Section: Discussionmentioning

confidence: 99%

Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán Argentina

D’Arpino,

Sineli,

Goroso

et al. 2023

Preprint

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Epidemiology based on the detection of pathogens in wastewater is extremely useful in providing information about a population’s health status. This study aimed to analyze and report the epidemiological dynamics of SARS-CoV-2 in the province of Tucumán, Argentina during the second and third surges of COVID-19 between April 2021 and March 2022. The study aimed to quantify SARS-CoV-2 RNA in wastewater, correlating it with clinically reported COVID-19 cases. Wastewater samples (n=72) were collected from 16 sampling points located in 3 cities of Tucumán (San Miguel de Tucumán, Yerba Buena y Banda del Río Salí). Detection of viral nucleocapsid markers (N1 gene) was carried out using one-step RT-qPCR. Viral loads were determined for each positive sample using a standard curve. A positive correlation (p<0.05) was observed between viral load (copies/mL) and the clinically confirmed COVID-19 cases reported during the sampling period in San Miguel de Tucumán. Our research findings provided a crucial insight into the dynamics of SARS-CoV-2 infection during epidemic outbreaks. The implementation of wastewater monitoring has proven to be an invaluable epidemiological tool, facilitating early detection of potential surges in COVID-19 cases, and enabling a comprehensive tracking of the pandemic. Our study underscores the significance of incorporating SARS-CoV-2 genome-based surveillance as a standard practice which will contribute to anticipating any future spikes in infections.

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Tracking SARS-CoV-2 in rivers as a tool for epidemiological surveillance

Cited by 10 publications

References 83 publications

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Wastewater monitoring of SARS‐CoV‐2 gene for COVID‐19 epidemiological surveillance in Tucumán, Argentina

Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán Argentina

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