Tracking phototactic responses and modeling motility of Synechocystis sp. strain PCC6803

Burriesci, Matthew S.; Bhaya, Devaki

doi:10.1016/j.jphotobiol.2008.01.012

Cited by 42 publications

(70 citation statements)

References 19 publications

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“…For instance, motility, biofilm formation, and cell sedimentation of Synechocystis are dependent on both type IV pili and EPSs (Bhaya et al, 1999(Bhaya et al, , 2000Burriesci and Bhaya, 2008;Jittawuttipoka et al, 2013). Thus, ethylene may alter various physiological responses through alterations in extracellular characteristics via changes in EPSs and type IV pili.…”

Section: Discussionmentioning

confidence: 99%

“…Ethylene had no detectable effect on the transcript levels of these genes in SynEtr1DTM2 Synechocystis. Thus, ethylene and SynEtr1 may affect phototaxis by altering pilin biogenesis that could lead to alterations in adhesion to surfaces or aggregation, which are factors that affect rates of motility (Burriesci and Bhaya, 2008).…”

Section: Application Of Ethylene Affects Pilus Formationmentioning

confidence: 99%

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Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

2016

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Ethylene is a plant hormone that plays a crucial role in the growth and development of plants. The ethylene receptors in plants are well studied, and it is generally assumed that they are found only in plants. In a search of sequenced genomes, we found that many bacterial species contain putative ethylene receptors. Plants acquired many proteins from cyanobacteria as a result of the endosymbiotic event that led to chloroplasts. We provide data that the cyanobacterium Synechocystis (Synechocystis sp. PCC 6803) has a functional receptor for ethylene, Synechocystis Ethylene Response1 (SynEtr1). We first show that SynEtr1 directly binds ethylene. Second, we demonstrate that application of ethylene to Synechocystis cells or disruption of the SynEtr1 gene affects several processes, including phototaxis, type IV pilus biosynthesis, photosystem II levels, biofilm formation, and spontaneous cell sedimentation. Our data suggest a model where SynEtr1 inhibits downstream signaling and ethylene inhibits SynEtr1. This is similar to the inverse-agonist model of ethylene receptor signaling proposed for plants and suggests a conservation of structure and function that possibly originated over 1 billion years ago. Prior research showed that SynEtr1 also contains a light-responsive phytochrome-like domain. Thus, SynEtr1 is a bifunctional receptor that mediates responses to both light and ethylene. To our knowledge, this is the first demonstration of a functional ethylene receptor in a nonplant species and suggests that that the perception of ethylene is more widespread than previously thought.

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Section: Discussionmentioning

confidence: 99%

Section: Application Of Ethylene Affects Pilus Formationmentioning

confidence: 99%

Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

2016

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“…There are also reports of T4P-mediated "gliding" motility in the clostridia, notably C. perfringens and Clostridium beijerinckii (397). Motility can be random or directed; for example, the cyanobacterium Synechocystis uses its T4P to move toward light sources, while M. xanthus and P. aeruginosa twitch up phospholipid gradients (54,66,284). Motility, or pilus retraction, is an important trait for many pathogens, as piliated but retraction-deficient mutants are attenuated in virulence (48,107,166,280,428).…”

Section: Motilitymentioning

confidence: 99%

Type IV Pilin Proteins: Versatile Molecular Modules

Giltner

Nguyen

Burrows

2012

Microbiol Mol Biol Rev

398

519

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SUMMARYType IV pili (T4P) are multifunctional protein fibers produced on the surfaces of a wide variety of bacteria and archaea. The major subunit of T4P is the type IV pilin, and structurally related proteins are found as components of the type II secretion (T2S) system, where they are called pseudopilins; of DNA uptake/competence systems in both Gram-negative and Gram-positive species; and of flagella, pili, and sugar-binding systems in the archaea. This broad distribution of a single protein family implies both a common evolutionary origin and a highly adaptable functional plan. The type IV pilin is a remarkably versatile architectural module that has been adopted widely for a variety of functions, including motility, attachment to chemically diverse surfaces, electrical conductance, acquisition of DNA, and secretion of a broad range of structurally distinct protein substrates. In this review, we consider recent advances in this research area, from structural revelations to insights into diversity, posttranslational modifications, regulation, and function.

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“…In the spherical unicellular cyanobacterium Synechocystis sp. strain PCC6803 (here Synechocystis), twitching depends on TFP that are arranged around the cell surface, and reversals in the twitching direction are not observed (31,32). Chemotaxis signaling via a system named Tax was proposed to function to polarize TFP activity or modulate TFP localization around the cells to bias movement in the direction of incident light (33).…”

Section: Chemotaxis Signaling and Motility Apparatusmentioning

confidence: 99%

“…Microscopically, these aggregates correspond to groups of motile cells that move at a greater speed than that of smaller aggregates or single cells (31,33). Aggregate sizes are initially variable, suggesting that initial cell-cell contacts are transient and dynamic, with some aggregates becoming sufficiently large to form finger-like projections (31,70). The role of chemotaxis in regulating aggregation is illustrated by the observation that mutations in tax genes cause a null phototaxis phenotype due to a lack of aggregation and failure to form fingerlike projections (22).…”

Section: Chemotaxis Control Of Twitching and Aggregation In Cyanobactmentioning

confidence: 99%

Chemotaxis Control of Transient Cell Aggregation

Alexandre

2015

J Bacteriol

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Tracking phototactic responses and modeling motility of Synechocystis sp. strain PCC6803

Cited by 42 publications

References 19 publications

Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

Ethylene Regulates the Physiology of the Cyanobacterium Synechocystis sp. PCC 6803 via an Ethylene Receptor

Type IV Pilin Proteins: Versatile Molecular Modules

Chemotaxis Control of Transient Cell Aggregation

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