Tracking novel adenovirus in environmental and human clinical samples: no evidence of endemic human adenovirus type 58 circulation in Córdoba city, Argentina

Ferreyra, Leonardo; Giordano, Miguel O.; Martı́nez, Laura C.; Barril, Patricia A.; Masachessi, Gisela; Isa, Marı́a Beatrı́z; Poma, R.; Rajal, Verónica Beatriz; Biganzoli, Patricia; Nates, Silvia; Pavan, Jorge Victorio

doi:10.1017/s0950268814002192

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…This creates a risk to human health via waterborne enteric viruses. A high occurrence of infections, produced by NoV, group A RV (RVA), HAV, HEV, and human EV, has been reported recently in Argentina in a variety of water environment matrices (3)(4)(5)(6)(7)(8).…”

Section: Importancementioning

confidence: 99%

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Masachessi

Pisano

Prez

et al. 2018

Appl Environ Microbiol

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Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment. The study shows the impact of anthropic contamination of one of the most important tourist water resources in Argentina. This course of recreational water would be a favorable scenario for infection, as well as a reservoir for the enteric viruses, creating a risk for the population exposed to these waters. The results obtained could alert the health systems and environmental sanitation to make decisions for the control and prevention of viral diseases in this environment.

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Section: Importancementioning

confidence: 99%

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Masachessi

Pisano

Prez

et al. 2018

Appl Environ Microbiol

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“…Adenovirus belongs to the family Adenoviridae and the genus Mastadenovirus. There are 58 types of adenoviruses identified to date, which are grouped into 7 species, A to G. They are grouped based on neutralisation tests or genome analysis (8).…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea

Çolak¹,

Bozdayı²,

Altay³

et al. 2017

Turk J Med Sci

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Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons.Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method.Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing. Conclusion:AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.

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“…Adenoviruses belong to the family Adenoviridae and genus Mastadenovirus . To date there are over 60 types of adenoviruses identified [ 9 ], grouped into seven species A to G on the basis of their resistance to neutralisation by antisera to other known human adenoviruses [ 10 ],[ 11 ] or genome analysis [ 11 ]. The disease pattern of adenoviruses varies according to species.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study

et al. 2014

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BackgroundHuman adenovirus (HAdV) causes acute diarrhoea sporadically, as well as in outbreaks. Understanding the prevalence and types of HAdV in diarrhoea is important for control and preventive measures, especially in the African region where there is a high burden of diarrhoeal disease. The present study assessed the prevalence, molecular characteristics, seasonality and associated clinical features of HAdV infection Tanzanian children below two years of age with and without diarrhoea between 2010–2011.MethodsStool specimens, demographic and clinical information were collected in 690 cases and 545 controls. All stool samples were screened for HAdV-antigen using ELISA. Positive samples subsequently underwent real-time PCR and sequencing for molecular typing.ResultsHAdV was detected in 37 children, corresponding to a prevalence of 3.5% (24/690) in diarrhoeic and 2.4% (13/545) in non-diarrhoeic children (P > 0.05). Among HAdV-infected children, the median age was significantly lower in diarrhoeic than in non-diarrhoeic children (10 vs. 14 months, P˂0.001). More than half of HAdV infected (54.2%) were dehydrated as compared to diarrhoeic children without HAdV (45.8%, P = 0.01). The proportion of the enteric HAdV type 40/41 in diarrhoeic and non-diarrhoeic children was (50.0%, 12/24) and (46.2%, 6/13) respectively. Other HAdV types detected were; 1, 2, 7, 18, 19 and 31. The prevalence of adenovirus was not significantly different between rainy and dry seasons. HAdV was not detected in the 33 known HIV positive children. There was no significant association between HAdV infection and gender, nutritional status of the child and parent educational level.ConclusionThe present study provides further evidence of the contribution of adenovirus in causing gastroenteritis in young children, with symptomatic infection being significantly more prevalent in children below one year. We found similar prevalence of adenovirus in non-diarrhoeic children and in diarrhoeic children. This first report on molecular epidemiology of human adenovirus in Tanzania observed diversity of HAdV types that circulate the study setting. The study findings suggest that HAdV is not an important cause of diarrhoea in young HIV-positive children.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-014-0666-1) contains supplementary material, which is available to authorized users.

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Tracking novel adenovirus in environmental and human clinical samples: no evidence of endemic human adenovirus type 58 circulation in Córdoba city, Argentina

Cited by 22 publications

References 28 publications

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea

Prevalence and molecular characterisation of human adenovirus in diarrhoeic children in Tanzania; a case control study

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