2010
DOI: 10.1039/c0an00509f
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Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy

Abstract: Aimed at developing accurate, reliable and cost-saving analytical techniques for drugs screening we evaluated the potential of Fourier Transform (FT) InfraRed (IR) microspectroscopy (microFTIR) as a quantitative pre-diagnostic approach for the rapid identification of IR signatures of drugs targeting specific molecular pathways causing Chronic Myeloid Leukemia (CML). To obtain reproducible FTIR absorbance spectra at the necessary spatial resolution we optimized sample preparation and acquisition parameters on a… Show more

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Cited by 89 publications
(112 citation statements)
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“…[92,106] As such, recent attention has been focussed on the use of SR-FTIR microspectroscopy for the study of biomolecular changes that occur following the administration of drugs or potential therapeutic agents in diseased cells. [92,[108][109][110][111][112] Importantly, macromolecules associated with biological samples produce signature IR bands that can be readily discerned from the IR spectra. [94,99,[113][114] Typical spectral features include: amide I ( 1650 cm 1 ) and amide II ( 1549 cm 1 ) bands representative of proteins; symmetric (1080 cm 1 ) and anti-symmetric PO 2  (1232 cm 1 ) bands associated with DNA; C-H stretches of -CH 3 ( as 2955 cm 1 ) and -CH 2 ( as 2850 cm 1 ), the ester band (1740 cm 1 ) and the C-O-P (1080 cm 1 ) band associated with lipids/phospholipids; and C-O stretches (1050 cm 1 ) of carbohydrates.…”
Section: Synchrotron Radiation -Fourier Transform Infrared Microspectmentioning
confidence: 99%
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“…[92,106] As such, recent attention has been focussed on the use of SR-FTIR microspectroscopy for the study of biomolecular changes that occur following the administration of drugs or potential therapeutic agents in diseased cells. [92,[108][109][110][111][112] Importantly, macromolecules associated with biological samples produce signature IR bands that can be readily discerned from the IR spectra. [94,99,[113][114] Typical spectral features include: amide I ( 1650 cm 1 ) and amide II ( 1549 cm 1 ) bands representative of proteins; symmetric (1080 cm 1 ) and anti-symmetric PO 2  (1232 cm 1 ) bands associated with DNA; C-H stretches of -CH 3 ( as 2955 cm 1 ) and -CH 2 ( as 2850 cm 1 ), the ester band (1740 cm 1 ) and the C-O-P (1080 cm 1 ) band associated with lipids/phospholipids; and C-O stretches (1050 cm 1 ) of carbohydrates.…”
Section: Synchrotron Radiation -Fourier Transform Infrared Microspectmentioning
confidence: 99%
“…The interest in the use of this technique stems from an urgent demand for the development of accurate and cost-saving analytical techniques that can be applied to the screening of new drug candidates. [109] The analysis involves the collection of spectra from large numbers of cells (generally >100 cells per sample) following exposure to the therapeutic agent, and the objective analysis of the resultant data through statistical procedures such as principal component analysis (PCA) and hierarchical cluster analysis. [92] While changes in spectral features can often be visually observed in average spectra, tools such as PCA remove subjectivity and add credence to the evaluation of any biomolecular differences/similarities in the spectra of cell or tissue samples.…”
Section: Synchrotron Radiation -Fourier Transform Infrared Microspectmentioning
confidence: 99%
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