Tracking Follicle Cell Development

Soriano, Adrianna; Petit, Christopher J.; Ryan, Savannah; Jemc, Jennifer C.

doi:10.1007/978-1-0716-2970-3_8

Cited by 1 publication

(1 citation statement)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Testes from w 1118 (PhLP3 +/+ ) and PhLP3 -/- males were dissected and processed for fluorescent in situ hybridization combined with immunostaining as described previously [44]. Probes were prepared by Molecular Instruments and were conjugated to AlexaFluor 488.…”

Section: Methodsmentioning

confidence: 99%

The evolutionarily conserved PhLP3 is essential for sperm development inDrosophila melanogaster

Petit,

Kojak,

Webster

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Phosducin-like proteins (PhLP) are thioredoxin domain-containing proteins that are highly-conserved across unicellular and multicellular organisms. PhLP family proteins are hypothesized to function as co-chaperones in the folding of cytoskeletal proteins. Here, we present the initial molecular, biochemical, and functional characterization ofCG4511asDrosophila melanogaster PhLP3. We cloned the gene into a bacterial expression vector and produced enzymatically active recombinant PhLP3, which showed similar kinetics to previously characterized orthologues. A fly strain homozygous for a P-element insertion in the 5’ UTR of thePhLP3gene exhibited significant downregulation ofPhLP3expression. We found these male flies to be sterile. Microscopic analysis revealed altered testes morphology and impairment of spermiogenesis, leading to a lack of mature sperm. Among the most significant observations was the lack of actin cones during sperm maturation. Excision of the P-element insertion inPhLP3restored male fertility, spermiogenesis, and seminal vesicle size. Given the high level of conservation of PhLP3, our data suggests PhLP3 may be an important regulator of sperm development across species.

show abstract

Section: Methodsmentioning

confidence: 99%