Tracking down the elusive early endosome

Lam, Sheung Kwan; Tse, Yu Chung; Robinson, David G.; Jiang, Liwen

doi:10.1016/j.tplants.2007.09.001

Cited by 91 publications

(110 citation statements)

References 77 publications

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“…Another variable to consider is changes in redoxosome size; our estimates for the flux of O 2 -are based on a diameter of 200 nm (Table 1). This estimate is consistent with the literature for early endosomes (7,8,28,43) and with our own studies evaluating redoxosomes by EM (42). However, endosome size is very dynamic and changes rapidly as endosomes traffic and fuse with other compartments.…”

Section: Lane 5 Vs 8) No Il-1r1=supporting

confidence: 92%

“…Using this method, a standard curve of peak height vs. nanomolar DMPO-OH was generated. Although these short scan times for experimental samples gave rise to lower signal-to-noise ratios than previously observed for isolated redoxosomes produced by longer scan times that average a greater number of spectra (28), the shorter scan times were necessary to minimize potential loss of the DMPO-OH adduct due to unknown enzymatic activity present in the samples.…”

Section: Calculations Of Endosomal Loss During Isolation Proceduresmentioning

confidence: 84%

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Endosomal Nox2 Facilitates Redox-Dependent Induction of NF-κB by TNF-α

Spencer

Oakley

et al. 2009

Antioxidants & Redox Signaling

105

133

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Growing evidence suggests that NADPH oxidase (Nox)-derived reactive oxygen species (ROS) play important roles in regulating cytokine signaling. We have explored how TNF-a induction of Nox-dependent ROS influences NF-kB activation. Cellular stimulation by TNF-a induced NADPH-dependent superoxide production in the endosomal compartment, and this ROS was required for IKK-mediated activation of NF-kB. Inhibiting endocytosis reduced the ability of TNF-a to induce both NADPH-dependent endosomal superoxide and NF-kB, supporting the notion that redox-dependent signaling of the receptor occurs in the endosome. Molecular analyses demonstrated that endosomal H 2 O 2 was critical for the recruitment of TRAF2 to the TNFR1=TRADD complex after endocytosis. Studies using both Nox2 siRNA and Nox2-knockout primary fibroblasts indicated that Nox2 was critical for TNF-a-mediated induction of endosomal superoxide. Redox-active endosomes that form after TNF-a or IL-1b induction recruit several common proteins (Rac1, Nox2, p67 phox , SOD1), while also retaining specificity for ligand-activated receptor effectors. Our studies suggest that TNF-a and IL-1b signaling pathways both can use Nox2 to facilitate redox activation of their respective receptors at the endosomal level by promoting the redox-dependent recruitment of TRAFs. These studies help to explain how cellular compartmentalization of redox signals can be used to direct receptor activation from the plasma membrane.

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Section: Lane 5 Vs 8) No Il-1r1=supporting

confidence: 92%

Section: Calculations Of Endosomal Loss During Isolation Proceduresmentioning

confidence: 84%

Endosomal Nox2 Facilitates Redox-Dependent Induction of NF-κB by TNF-α

Spencer

Oakley

et al. 2009

Antioxidants & Redox Signaling

105

133

View full text Add to dashboard Cite

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“…In plant cells, most vacuolar proteins are sorted at the TGN and transported to the vacuole through PVCs (Lam et al, 2007a). However, the underlying mechanism of PVC-to-vacuole transport is largely unknown in plants.…”

Section: The Mon1-ccz1 Complex Mediates Rab5-to-rab7 Conversion On Pvmentioning

confidence: 99%

Activation of the Rab7 GTPase by the MON1-CCZ1 Complex Is Essential for PVC-to-Vacuole Trafficking and Plant Growth in Arabidopsis

et al. 2014

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“…Previous studies suggested that the TGN serves as a major sorting station for biosynthetic cargos in plants (Lam et al, 2007a;Robinson et al, 2008). Cytological evidence from pea (Pisum sativum) and Arabidopsis thaliana show that sorting of storage proteins occurs early in the cis-cisternae of the Golgi apparatus (Hillmer et al, 2001;Otegui et al, 2006), where two types of putative vacuolar sorting determinants (VSRs), the BP-80 family members and the receptor homology region transmembrane domain RING H2 MOTIF PROTEIN1 (RMR1), most probably act as receptors for soluble storage proteins (Robinson et al, 2005;Shen et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

et al. 2014

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In seed plants, a major pathway for sorting of storage proteins to the protein storage vacuole (PSV) depends on the Golgi-derived dense vesicles (DVs). However, the molecular mechanisms regulating the directional trafficking of DVs to PSVs remain largely elusive. Here, we report the functional characterization of the rice (Oryza sativa) glutelin precursor accumulation3 (gpa3) mutant, which exhibits a floury endosperm phenotype and accumulates excess proglutelins in dry seeds. Cytological and immunocytochemistry studies revealed that in the gpa3 mutant, numerous proglutelin-containing DVs are misrouted to the plasma membrane and, via membrane fusion, release their contents into the apoplast to form a new structure named the paramural body. Positional cloning of GPA3 revealed that it encodes a plant-specific kelch-repeat protein that is localized to the trans-Golgi networks, DVs, and PSVs in the developing endosperm. In vitro and in vivo experiments verified that GPA3 directly interacts with the rice Rab5a-guanine exchange factor VPS9a and forms a regulatory complex with Rab5a via VPS9a. Furthermore, our genetic data support the notion that GPA3 acts synergistically with Rab5a and VPS9a to regulate DV-mediated post-Golgi traffic in rice. Our findings provide insights into the molecular mechanisms regulating the plant-specific PSV pathway and expand our knowledge of vesicular trafficking in eukaryotes.

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Tracking down the elusive early endosome

Cited by 91 publications

References 77 publications

Endosomal Nox2 Facilitates Redox-Dependent Induction of NF-κB by TNF-α

Endosomal Nox2 Facilitates Redox-Dependent Induction of NF-κB by TNF-α

Activation of the Rab7 GTPase by the MON1-CCZ1 Complex Is Essential for PVC-to-Vacuole Trafficking and Plant Growth in Arabidopsis

GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

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