Tracking down the different forms of nuclear actin

Jockusch, Brigitte M.; Schoenenberger, Cora‐Ann; Stetefeld, Jörg; Aebi, Ueli

doi:10.1016/j.tcb.2006.06.006

Cited by 93 publications

(79 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The most conspicuous cytoplasmic actin cytoskeleton in plant cells is essential for a wide range of cellular processes, including establishing and maintaining cell shape and polarity, tip growth, cytoplasmic streaming, organelle movement and repositioning, cell division, and responses to external signals (reviewed in Wasteneys and Galway, 2003;Smith and Oppenheimer, 2005;Hussey et al, 2006;Staiger and Blanchoin, 2006). In addition, as in vertebrates (Jockusch et al, 2006;McDonald et al, 2006;Vartiainen, 2008), actin has also been identified in the nucleus of plant cells (Skubatz et al, 2000;Cruz and Moreno Díaz de la Espina, 2009). The nuclear actin along with actin-related proteins and a host of other nuclear proteins is implicated in diverse nuclear processes, such as chromatin remodeling, regulation of gene expression, transcription, RNA processing, and nuclear export (Blessing et al, 2004;Miralles and Visa, 2006;Meagher et al, 2007;Vartiainen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for NormalArabidopsisDevelopment

2009

View full text Add to dashboard Cite

The relative significance of gene regulation and protein isovariant differences remains unexplored for most gene families, particularly those participating in multicellular development. Arabidopsis thaliana encodes three vegetative actins, ACT2, ACT7, and ACT8, in two ancient and highly divergent subclasses. Mutations in any of these differentially expressed actins revealed only mild phenotypes. However, double mutants were extremely dwarfed, with altered cell and organ morphology and an aberrant F-actin cytoskeleton (e.g., act2-1 act7-4 and act8-2 act7-4) or totally root-hairless (e.g., act2-1 act8-2). Our studies suggest that the three vegetative actin genes and protein isovariants play distinct subclass-specific roles during plant morphogenesis. For example, during root development, ACT7 was involved in root growth, epidermal cell specification, cell division, and root architecture, and ACT2 and ACT8 were essential for root hair tip growth. Also, genetic complementation revealed that the ACT2 and ACT8 isovariants, but not ACT7, fully rescued the root hair growth defects of single and double mutants. Moreover, we synthesized fully normal plants overexpressing the ACT8 isovariant from multiple actin regulatory sequences as the only vegetative actin in the act2-1 act7-4 background. In summary, it is evident that differences in vegetative actin gene regulation and the diversity in actin isovariant sequences are essential for normal plant development.

show abstract

Section: Introductionmentioning

confidence: 99%

A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for NormalArabidopsisDevelopment

2009

View full text Add to dashboard Cite

show abstract

“…The presence of ␤-actin in the nucleus was documented for the first time many years ago (Egly et al, 1984;Scheer et al, 1984), but the connection between ␤-actin and gene transcription has only recently been demonstrated (Olave et al, 2002;Pederson and Aebi, 2002;Fomproix and Percipalle, 2004;Grummt, 2006;Jockusch et al, 2006;Percipalle and Visa, 2006;Obrdlik et al, 2007). Nuclear ␤-actin associates with components of the ATP-dependent chromatin-remodeling complexes (Olave et al, 2002;Bettinger et al, 2004), with RNP particles (Percipalle and Visa, 2006), and with the three RNA polymerases in the eukaryotic cell nucleus, both in vitro and in vivo (Hu et al, 2004;Philimonenko et al, 2004;Kukalev et al, 2005;Wu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

Ferrai

Naum-Onganía

Longobardi

et al. 2009

MBoC

View full text Add to dashboard Cite

We have analyzed the role of actin polymerization in retinoic acid (RA)-induced HoxB transcription, which is mediated by the HoxB regulator Prep1. RA induction of the HoxB genes can be prevented by the inhibition of actin polymerization. Importantly, inhibition of actin polymerization specifically affects the transcription of inducible Hox genes, but not that of their transcriptional regulators, the RARs, nor of constitutively expressed, nor of actively transcribed Hox genes. RA treatment induces the recruitment to the HoxB2 gene enhancer of a complex composed of "elongating" RNAPII, Prep1, ␤-actin, and N-WASP as well as the accessory splicing components p54Nrb and PSF. We show that inhibition of actin polymerization prevents such recruitment. We conclude that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes.

show abstract

“…The fact that actin can also be found within the nucleus was long seen as controversial, since nuclear F-actin cannot be made visible cytochemically by phalloidin. In recent years, however, it became clear that nuclear actin can be found in many cell types as well as in neurons [2,10,18,25]. Moreover, a multitude of ABPs such as profilin, cofilin, thymosin [2,8,25] and actin related proteins (ARPs) [16] localise to the nucleus, suggesting dynamic regulation of nuclear actin.…”

Section: Excursus: Nuclear Actinmentioning

confidence: 99%

The cytoskeleton and nucleus: the role of actin as a modulator of neuronal gene expression

Knöll

Beck

2011

E-Neuroforum

View full text Add to dashboard Cite

Actin, arranged for example in stress fibres, provides a fundamental cytoskeletal framework function to all cell types. Notably, there is now mounting evidence that, in addition to cytoplasmic cytoskeletal regulation, actin treadmilling provides a signal modulating nuclear gene expression. In altering gene regulation, cytoplasmic and most likely also a nucleus-resident actin provides an additional (gene) regulatory twist to cell motility. So far, the transcription factor serum response factor (SRF) alongside its myocardin-related transcription factor (MRTF) cofactors has emerged as the main target of actin dynamics. In this review, we discuss the impact of actin signalling on nuclear gene expression in the nervous system, where the actin-MRTF-SRF module contributes to various processes including neuronal motility.

show abstract

Tracking down the different forms of nuclear actin

Cited by 93 publications

References 65 publications

A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for NormalArabidopsisDevelopment

A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for NormalArabidopsisDevelopment

Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization

The cytoskeleton and nucleus: the role of actin as a modulator of neuronal gene expression

Contact Info

Product

Resources

About