Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

Turk, Madison; Naumenko, Victor; Mahoney, Douglas J.; Jenne, Craig N.

doi:10.3390/cells7070069

Cited by 12 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within tumour vessels intravital imaging approaches to visualise the leucocyte-endothelium interactions, Turk and co-workers identified few crawling Ly6G + neutrophils in the venules while CD8 + T cells crawled in both collecting and post-capillary venules, indicating different leucocyte subpopulations may crawl along distinct vascular structures or components within the TME (extracellular matrix, collagen, etc.) [ 75 ]. Further, it was also revealed that intratumoral leucocytes of both lymphoid and myeloid origin showed an active non-altered intraluminal migration in the mouse hepatocellular cancer model, and the locomotion velocity of leucocytes was also cell number-independent [ 76 ].…”

Section: Leucocyte Traffickingmentioning

confidence: 99%

The Tumour Vasculature as a Target to Modulate Leucocyte Trafficking

Zhao

Ting

Coleman

et al. 2021

Cancers

View full text Add to dashboard Cite

The effectiveness of immunotherapy against solid tumours is dependent on the appropriate leucocyte subsets trafficking and accumulating in the tumour microenvironment (TME) with recruitment occurring at the endothelium. Such recruitment involves interactions between the leucocytes and the endothelial cells (ECs) of the vessel and occurs through a series of steps including leucocyte capture, their rolling, adhesion, and intraluminal crawling, and finally leucocyte transendothelial migration across the endothelium. The tumour vasculature can curb the trafficking of leucocytes through influencing each step of the leucocyte recruitment process, ultimately producing an immunoresistant microenvironment. Modulation of the tumour vasculature by strategies such as vascular normalisation have proven to be efficient in facilitating leucocyte trafficking into tumours and enhancing immunotherapy. In this review, we discuss the underlying mechanisms of abnormal tumour vasculature and its impact on leucocyte trafficking, and potential strategies for overcoming the tumour vascular abnormalities to boost immunotherapy via increasing leucocyte recruitment.

show abstract

Section: Leucocyte Traffickingmentioning

confidence: 99%

The Tumour Vasculature as a Target to Modulate Leucocyte Trafficking

Zhao

Ting

Coleman

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…The most powerful research tool available to characterize neutrophil trafficking in vivo is intravital microscopy (IVM), which involves surgical exposure of an organ of interest (in a live anesthetized animal) and direct visualization with a microscope to observe neutrophil behavior in blood vessels and tissues [ 5 ]. Within the field of immuno-oncology, IVM has proven particularly powerful to study the trafficking and function of neutrophils within tumors as well as their contribution to cancer metastasis [ 6 , 7 , 8 , 9 ]. In this section, we provide a primer overview of the most common contemporary IVM modalities (confocal and two-photon imaging) and explore emerging IVM technologies that will expand our ability to discover new aspects of neutrophil biology in vivo.…”

Section: Seeing Is Believing—in Vivo Imaging Of Neutrophil Trafficking and Functionmentioning

confidence: 99%

A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond

Changirwa

Schlechte

McDonald

2021

Cancers

View full text Add to dashboard Cite

As key effector cells of the innate immune response, neutrophils are rapidly deployed to sites of inflammation where they deliver a payload of potent effector mechanisms that are essential for host defense against pathogens as well as tissue homeostasis. In addition, neutrophils are central contributors to the pathogenesis of a vast spectrum of inflammatory, degenerative, and neoplastic diseases. As our understanding of neutrophils in health and disease continually expands, so too does our appreciation of their complex and dynamic nature in vivo; from development, maturation, and trafficking to cellular heterogeneity and functional plasticity. Therefore, contemporary neutrophil research relies on multiple complementary methodologies to perform integrated analysis of neutrophil phenotypic heterogeneity, organ- and stimulus-specific trafficking mechanisms, as well as tailored effector functions in vivo. This review discusses established and emerging technologies used to study neutrophils, with a focus on in vivo imaging in animal models, as well as next-generation ex vivo model systems to study mechanisms of neutrophil function. Furthermore, we discuss how high-dimensional single-cell analysis technologies are driving a renaissance in neutrophil biology by redefining our understanding of neutrophil development, heterogeneity, and functional plasticity. Finally, we discuss innovative applications and emerging opportunities to integrate these high-dimensional, multi-modal techniques to deepen our understanding of neutrophils in cancer research and beyond.

show abstract

“…When pairing these fluorescent transgenic mice with an intravital microscope, it becomes possible to delve deep underneath dense tissue and potentially observe megakaryopoiesis and changes in ploidy and proplatelet formation and release into the blood stream (87,164). Fluorescently-labeled platelets have enabled studies of platelet migration and platelet interactions with other blood cells, in a number of physiology scenarios, including inflammation (139), infection (165), and cancer (166).…”

Section: Hemostasis and Thrombus Formation In Its Natural Microenviromentioning

confidence: 99%

“…The concept of in vivo IFC systems was developed to count, characterize, and image biological cells flowing in a living organism (in this case a mouse) at different time points, thus providing longitudinal information of biological events. The in vivo flow cytometer was first used to quantify the circulation lifetime of different tumor cells and monitor apoptotic cells in circulation (166,207,208) with single cell sensitivity. However, existing in vivo IFC approaches are limited by technological challenges that restrict 2D regions of interest to superficial layers of tissue, preventing the experimental interrogation of cellular and molecular events in major blood vessels.…”

Section: In Vivo Imaging Cytometrymentioning

confidence: 99%

Imaging Platelet Processes and Function—Current and Emerging Approaches for Imaging in vitro and in vivo

et al. 2020

View full text Add to dashboard Cite

Platelets are small anucleate cells that are essential for many biological processes including hemostasis, thrombosis, inflammation, innate immunity, tumor metastasis, and wound healing. Platelets circulate in the blood and in order to perform all of their biological roles, platelets must be able to arrest their movement at an appropriate site and time. Our knowledge of how platelets achieve this has expanded as our ability to visualize and quantify discreet platelet events has improved. Platelets are exquisitely sensitive to changes in blood flow parameters and so the visualization of rapid intricate platelet processes under conditions found in flowing blood provides a substantial challenge to the platelet imaging field. The platelet's size (∼2 µm), rapid activation (milliseconds), and unsuitability for genetic manipulation, means that appropriate imaging tools are limited. However, with the application of modern imaging systems to study platelet function, our understanding of molecular events mediating platelet adhesion from a single-cell perspective, to platelet recruitment and activation, leading to thrombus (clot) formation has expanded dramatically. This review will discuss current platelet imaging techniques in vitro and in vivo, describing how the advancements in imaging have helped answer/expand on platelet biology with a particular focus on hemostasis. We will focus on platelet aggregation and thrombus formation, and how platelet imaging has enhanced our understanding of key events, highlighting the knowledge gained through the application of imaging modalities to experimental models in vitro and in vivo. Furthermore, we will review the limitations of current imaging techniques, and questions in thrombosis research that remain to be addressed. Finally, we will speculate how the same imaging advancements might be applied to the imaging of other vascular cell biological functions and visualization of dynamic cell-cell interactions.

show abstract

Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

Cited by 12 publications

References 46 publications

The Tumour Vasculature as a Target to Modulate Leucocyte Trafficking

The Tumour Vasculature as a Target to Modulate Leucocyte Trafficking

A Multi-Modal Toolkit for Studying Neutrophils in Cancer and Beyond

Imaging Platelet Processes and Function—Current and Emerging Approaches for Imaging in vitro and in vivo

Contact Info

Product

Resources

About