Track A

Frey, Christoph; Sales, Adrià; Athanasopulu, Kiriaki; Spatz, Joachim P.; Kemkemer, Ralf

doi:10.1515/bmt-2014-5000

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…Results that do exist for silicone electrospinning have shown fiber diameters of approximately 20 μm, well above the desired range of the BVM lab 170 .…”

Section: Silicone (Medical Grade)mentioning

confidence: 85%

Identifying and Reducing Variability, Improving Scaffold Morphology, and Investigating Alternative Materials for the Blood Vessel Mimic Lab Electrospinning Process

Dowey¹

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The work of the Cal Poly Tissue Engineering Lab is primarily focused on the fabrication, characterization, and improvement of "Blood Vessel Mimics" (BVMs), tissue engineered constructs used to evaluate cellular response to vascular medical devices. Currently, cells are grown onto fibrous, porous tubes made using an in-house electrospinning process from PLGA, a biocompatible co-polymer. The adhesion and proliferation of cells in a BVM is reliant on the micro-scale structure of the PLGA scaffold, and as such it is of great importance for the electrospinning process to consistently produce scaffolds of similar morphologies. Additionally, it has been shown that cell proliferation increases with scaffolds of smaller fibers and pores than the current electrospinning protocol can produce. Finally, the Tissue Engineering Lab has interest in testing devices in more tortuous BVM bioreactor designs, however the use of relatively rigid PLGA scaffolds has severely limited the ability to construct more complicated vessel geometries. v protocol were identified and subsequently altered to improve consistency and usability. Specifically, this included redefining the precision with which PLGA mass was measured, repositioning electrical equipment to reduce the effect of stray electrostatic forces on the polymer solution jet, attempting to control the temperature and humidity inside the electrospinning enclosure, and improving the ease with which scaffolds are removed from their mandrels through alternative mandrel surface treatments. In addition to overall process variability, the issue of scaffold shrinkage during BVM use was investigated and two possible treatments, exposure to either ethanol or elevated temperatures, were proposed based on previous electrospinning literature results. Each was tested for their effectiveness in mitigating shrinkage through exposure to BVM setup-mimicking conditions. It was found that both treatments reduced scaffold shrinkage compared to control samples when exposed to BVM setup-mimicking conditions. Finally, 3 flexible polymers were selected and electrospun to compare against typical PLGA results and to conduct a kink radius test as a metric for measuring flexibility as it pertains to the proposed BVM lab application. It was concluded that two types of thermoplastic polyurethane (tPU) were not acceptable electrospinning materials for use in the BVM lab. Additionally, while polycaprolactone (PCL) could be successfully electrospun it could not undergo the amount bending required for more tortuous BVM bioreactor designs without kinking.Overall, the work in this thesis provided insight into multiple scaffold characterization techniques, reduced overall electrospinning variability in the fabrication and use of PLGA scaffolds, and defined processing parameters that have been shown to yield scaffolds with smaller morphological features than all prior Tissue Engineering Lab work. By creating better, more effective scaffolds, researchers in the Tissue Engineering Lab can more accurately mimic the structur...

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“…Results that do exist for silicone electrospinning have shown fiber diameters of approximately 20 μm, well above the desired range of the BVM lab 170 .…”

Section: Silicone (Medical Grade)mentioning

confidence: 85%

Identifying and Reducing Variability, Improving Scaffold Morphology, and Investigating Alternative Materials for the Blood Vessel Mimic Lab Electrospinning Process

Dowey¹

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Camouflaged angiogenic BMP-2 functions exposed by pico-paracrine biohybrids

Jennissen

2023

Front. Bioeng. Biotechnol.

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The constant release of human bone morphogenetic protein 2 (rhBMP-2) in the picomolar range (Pico-Stat) from PDLLA-biohybrids led to the detection of intrinsic novel pro- and anti-angiogenic functions of this cytokine. As integrant part in this perspective of previous work, first evidence for the binding of rhBMP-2, as an inverse agonist, to allosteric angiogenic receptors in cocultures of human endothelial cells is reported.

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Track A

Cited by 2 publications

References 20 publications

Identifying and Reducing Variability, Improving Scaffold Morphology, and Investigating Alternative Materials for the Blood Vessel Mimic Lab Electrospinning Process

Identifying and Reducing Variability, Improving Scaffold Morphology, and Investigating Alternative Materials for the Blood Vessel Mimic Lab Electrospinning Process

Camouflaged angiogenic BMP-2 functions exposed by pico-paracrine biohybrids

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