Tracing Transduced Cells in Osteochondral Defects

Milbrandt, Todd A.; Berthoux, Lionel; Christenson, Vernon; Baumbusch, Clark; Rekosh, David; Balian, Gary; Diduch, David R.

doi:10.1097/01241398-200307000-00003

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…Transplanted MSCs in chondral defects showed that they decreased in number with time 18 . Although the cells might have lost the ability to produce GFP, their disappearance is more likely 19 . Other groups reported that transplanted cells survived for 10 days to 8 weeks in chondral defects 20,21 .…”

Section: Discussionmentioning

confidence: 99%

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Behavior of Transplanted Bone Marrow–Derived Mesenchymal Stem Cells in Periodontal Defects

Hasegawa

Kawaguchi

Hirachi

et al. 2006

Journal of Periodontology

157

132

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Section: Discussionmentioning

confidence: 99%

“…18 Although the cells might have lost the ability to produce GFP, their disappearance is more likely. 19 Other groups reported that transplanted cells survived for 10 days to 8 weeks in chondral defects. 20,21 Additional studies with short-and long-term observations will extend our knowledge on the exact fate of the transplanted MSCs.…”

Section: Discussionmentioning

confidence: 99%

Behavior of Transplanted Bone Marrow–Derived Mesenchymal Stem Cells in Periodontal Defects

Hasegawa

Kawaguchi

Hirachi

et al. 2006

Journal of Periodontology

157

132

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“…Transduction efficiencies reached up to 50% with adenoviral vectors [69] but transgene expression was seen only for a short period of time. Cell selection is generally a prerequisite when using retroviral vectors [72], although enhanced efficiencies have been reported with newly generated constructs [73]. Promising results were obtained based on the use of recombinant adeno-associated viral (rAAV) vectors [74][75][76].…”

Section: Gene Transfer Into Progenitor Cells In Vitromentioning

confidence: 99%

Gene therapy for cartilage defects

Cucchiarini

Madry

2005

The Journal of Gene Medicine

110

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Focal defects of articular cartilage are an unsolved problem in clinical orthopaedics. These lesions do not heal spontaneously and no treatment leads to complete and durable cartilage regeneration. Although the concept of gene therapy for cartilage damage appears elegant and straightforward, current research indicates that an adaptation of gene transfer techniques to the problem of a circumscribed cartilage defect is required in order to successfully implement this approach. In particular, the localised delivery into the defect of therapeutic gene constructs is desirable. Current strategies aim at inducing chondrogenic pathways in the repair tissue that fills such defects. These include the stimulation of chondrocyte proliferation, maturation, and matrix synthesis via direct or cell transplantation-mediated approaches. Among the most studied candidates, polypeptide growth factors have shown promise to enhance the structural quality of the repair tissue. A better understanding of the basic scientific aspects of cartilage defect repair, together with the identification of additional molecular targets and the development of improved gene-delivery techniques, may allow a clinical translation of gene therapy for cartilage defects. The first experimental steps provide reason for cautious optimism.

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“…33 EGFP-labeled allogeneic chondrocytes transplanted into a similar rabbit model could not be detected after 6 weeks anymore. 34 It was unclear whether a decrease in density of labeled cells was due to cell death, through rejection by the host cells, or due to loss of expression. Also, the process of transduction itself could have had an effect on the behavior of transplanted cells.…”

mentioning

confidence: 99%

In Vivo Matrix Production by Bone Marrow Stromal Cells Seeded on PLGA Scaffolds for Ligament Tissue Engineering

Eijk

Saris

Fedorovich

et al. 2009

Tissue Engineering Part A

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Ligament tissue engineering based on cell-seeded biomechanically functional constructs is a commonly studied strategy toward native anterior cruciate ligament replacement. Little is known about the survival and differentiation of the seeded cells after the transplantation. We applied retroviral genetic marking to trace implanted cells and studied their differentiation by species-specific immunolabeling of the extracellular matrix produced. Goat bone marrow stromal cells were transduced with a MoMuLV-based vector encoding the DeltaLNGFR gene. Transduced cells were seeded onto poly(lactic-co-glycolic acid) (PLGA) fibers and implanted subcutaneously into nude mice and left for various periods up to 6 weeks. Immunohistochemistry for LNGFR expression showed survival of the seeded cells after transplantation for up to 6 weeks. Immunohistochemistry for collagen type I and III showed the production of fibrous tissue inside the scaffolds. Moreover, using a goat-specific anti-collagen type III, donor-derived matrix could be demonstrated. We conclude that bone marrow stromal cells survived in vivo and at least partially differentiated after implantation.

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Tracing Transduced Cells in Osteochondral Defects

Cited by 11 publications

References 23 publications

Behavior of Transplanted Bone Marrow–Derived Mesenchymal Stem Cells in Periodontal Defects

Behavior of Transplanted Bone Marrow–Derived Mesenchymal Stem Cells in Periodontal Defects

Gene therapy for cartilage defects

In Vivo Matrix Production by Bone Marrow Stromal Cells Seeded on PLGA Scaffolds for Ligament Tissue Engineering

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