Tracing the skeletal progenitor transition during postnatal bone formation

Shu, Hui Sophie; Liu, Yiming Liam; Tang, Xinyu Thomas; Zhang, Xinyi Shirley; Zhou, Bin; Zou, Weiguo; Zhou, Bo

doi:10.1016/j.stem.2021.08.010

Cited by 96 publications

(98 citation statements)

References 35 publications

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“…Previous genetic lineage tracing studies have demonstrated that most HCs can survive at the chondro-osseous junction and further convert into OLCs, adipocytes, or bSMCs (Giovannone et al, 2019; Long et al, 2022; Mizuhashi et al, 2019; Mizuhashi et al, 2018; Park et al, 2015; Shu et al, 2021; Yang et al, 2014a; Yang et al, 2014b; Zhou et al, 2014b). Given that most HC progeny are enriched in the metaphysis, it is conceivable that HC is the cellular source of the putative SSC at the metaphysis.…”

Section: Resultsmentioning

confidence: 99%

Identification of the Metaphyseal Skeletal Stem Cell

Guan

et al. 2022

Preprint

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Identification of novel regional skeletal stem cells (SSCs) will provide a new cellular paradigm for bone physiology and dysfunction. Several populations of SSCs have been identified at distinct skeletal sites. However, a bona fide SSC population has not yet been formally characterized in the bone marrow. Here, we identify a metaphyseal SSCs (mpSSCs) population whose transcriptional landscape is distinct from other bone mesenchymal stromal cells (bMSCs) in the bone marrow. These mpSSCs emerge at the postnatal stage and reside just underneath the growth plate, consistent with the fact that these mpSSCs are exclusively derived from hypertrophic chondrocytes (HCs). These mpSSCs possess SSC properties such as self-renewal and multipotency in vitro and in vivo, stand at the top of the HC de-differentiation path, and produce most HC progeny. Genetic block of the conversion from HCs to mpSSCs significantly compromises trabecular bone formation and bone regeneration. Thus, metaphysis houses a unique HC-derived SSC population, which is a major source of osteoblasts and bMSCs supporting postnatal trabecular bone formation.

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Section: Resultsmentioning

confidence: 99%

Identification of the Metaphyseal Skeletal Stem Cell

Guan

et al. 2022

Preprint

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“…In addition, Chondrocytes and Lepr + BMSCs mediate longitudinal growth and transverse thickening of the bone before and after adolescence, respectively. ( Shu et al, 2021 ) reported that osteoblasts are mainly emerged from Acan + chondrocytes in the growth plate to realize bone lengthening before adolescence, whereas after adolescence, they primarily arise from LepR + BMSCs to achieve bone thickening.…”

Section: Scrna-seq-based Msc Identification and Function Assessmentmentioning

confidence: 99%

“…This technology has been applied to reveal the developmental origin of hepatic vasculature and the cell fate of club cells, AT2 cells and bronchoalveolar stem cells in the process of lung repair and regeneration ( Zhang et al, 2016 ; Liu et al, 2020 ). However, few MSC-related studies are using DeaLT technology ( Shu et al, 2021 ). The combined application of DeaL technology and single-cell technology will bring more precise and reliable interpretations on the heterogeneity of MSCs, and facilitate understanding of the regulatory network that controls cell state, which exhibits extensive application prospects and warrant further exploration.…”

Section: Scrna-seq On Cre-targeted Mscs In Transgenic Micementioning

confidence: 99%

Recent Advances in Single-Cell View of Mesenchymal Stem Cell in Osteogenesis

Shen

Shi

2022

Front. Cell Dev. Biol.

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Osteoblasts continuously replenished by osteoblast progenitor cells form the basis of bone development, maintenance, and regeneration. Mesenchymal stem cells (MSCs) from various tissues can differentiate into the progenitor cell of osteogenic lineage and serve as the main source of osteoblasts. They also respond flexibly to regenerative and anabolic signals emitted by the surrounding microenvironment, thereby maintaining bone homeostasis and participating in bone remodeling. However, MSCs exhibit heterogeneity at multiple levels including different tissue sources and subpopulations which exhibit diversified gene expression and differentiation capacity, and surface markers used to predict cell differentiation potential remain to be further elucidated. The rapid advancement of lineage tracing methods and single-cell technology has made substantial progress in the characterization of osteogenic stem/progenitor cell populations in MSCs. Here, we reviewed the research progress of scRNA-seq technology in the identification of osteogenic markers and differentiation pathways, MSC-related new insights drawn from single-cell technology combined with experimental technology, and recent findings regarding the interaction between stem cell fate and niche in homeostasis and pathological process.

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“…Leptin receptor (LepR)‐expressing bone marrow stromal cells have been validated as major source of osteoprogenitor cells in adult mice. They can give rise to most bone and adipocytes, and thus are responsible for adult long bone homeostasis maintaining as well as fracture healing (Shu et al, 2021; B. O. Zhou, Yue, Murphy, Peyer, & Morrison, 2014). In jaw bone, Zhang et al used a tooth extraction model to verify its role in alveolar bone healing (D. Zhang et al, 2020).…”

Section: In Vivo Identification Of Periodontal Stem Cellsmentioning

confidence: 99%

Advances in periodontal stem cells and the regulating niche: From in vitro to in vivo

Liu

Men

et al. 2022

Genesis

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Summary Periodontium possesses stem cell populations for its self‐maintenance and regeneration, and has been proved to be an optimal stem cell source for tissue engineering. In vitro studies have shown that stem cells can be isolated from periodontal ligament, alveolar bone marrow and gingiva. In recent years, more studies have focused on identification of periodontal stem cells in vivo. Multiple genetic markers, including Gli1, Prx1, Axin2, αSMA, and LepR, were identified with the lineage tracing approaches. Characteristics, functions, and regulatory mechanisms of specific populations expressing one of these markers have been investigated. In vivo studies also revealed that periodontal stem cells can be regulafrted by different niche and mechanisms including intercellular interactions, ECM and multiple secreted factors. In this review, we summarized the current knowledge of in vitro characteristics and in vivo markers of periodontal stem cells, and discussed the specific regulating niche.

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Tracing the skeletal progenitor transition during postnatal bone formation

Cited by 96 publications

References 35 publications

Identification of the Metaphyseal Skeletal Stem Cell

Identification of the Metaphyseal Skeletal Stem Cell

Recent Advances in Single-Cell View of Mesenchymal Stem Cell in Osteogenesis

Advances in periodontal stem cells and the regulating niche: From in vitro to in vivo

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