Tracing the <i>In Vivo</i> Fate of Nanoparticles with a “Non-Self” Biological Identity

Mohammad‐Beigi, Hossein; Scavenius, Carsten; Jensen, Pia Bomholt; Kjær-Sørensen, Kasper; Oxvig, Claus; Boesen, Thomas; Enghild, Jan J.; Sutherland, Duncan S.; Hayashi, Yuya

doi:10.1021/acsnano.0c05178

Cited by 13 publications

(10 citation statements)

References 60 publications

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“…[32] This suggests that the biological responses and therapeutic outcomes observed in vitro may not be directly translated to clinical use. [33][34] While similar effects of the protein source on corona composition have been reported and-at least in part-characterized, much less is known on potential effects related to the presence of excess free proteins in solution. Previous studies showed that the addition of human serum in the medium (as opposed to FBS) usually decreases nanoparticle uptake and a lower uptake is observed when serum concentration is increased.…”

Section: Introductionmentioning

confidence: 79%

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Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins

Yang

Reker‐Smit

Stuart

et al. 2021

Adv Healthcare Materials

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Corona formation in biological fluids strongly affects nanomedicine interactions with cells. However, relatively less is known on additional effects from the free proteins in solution. Within this context, this study aims to gain a better understanding of nanomaterial-cell interactions in different biological fluids and, more specifically, to disentangle effects due to corona composition and those from the free proteins in solution. To this aim, the uptake of liposomes in medium with bovine and human serum are compared. Uptake efficiency in the two media differs strongly, as also corona composition. However, in contrast with similar studies on other nanomaterials, despite the very different corona, when the two corona-coated liposomes are exposed to cells in serum free medium, their uptake is comparable. Thus, in this case, the observed differences in uptake depend primarily on the presence and source of the free proteins. Similar results are obtained when testing the liposomes on different human cells, as well as in murine cells and in the presence of murine serum. Overall, these results show that the protein source affects nanomedicine uptake not only due to effects on corona composition, but also due to the presence and composition of the free proteins in solution.

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Section: Introductionmentioning

confidence: 79%

“…[ 32 ] This suggests that the biological responses and therapeutic outcomes observed in vitro may not be directly translated to clinical use. [ 33–34 ]…”

Section: Introductionmentioning

confidence: 99%

Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins

Yang

Reker‐Smit

Stuart

et al. 2021

Adv Healthcare Materials

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“…There have been increasing evidences that nanomaterials can induce inflammatory responses by their conversion into protein corona complexes [ 29 – 31 ]. Therefore, we tested the proinflammatory effect of Fe-Cur NCPs on macrophages.…”

Section: Resultsmentioning

confidence: 99%

Ultrasmall Coordination Polymers for Alleviating ROS-Mediated Inflammatory and Realizing Neuroprotection against Parkinson’s Disease

Cheng

Liu

et al. 2022

Research

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Parkinson’s disease (PD) is the second most common neurodegenerative disease globally, and there is currently no effective treatment for this condition. Excessive accumulation of reactive oxygen species (ROS) and neuroinflammation are major contributors to PD pathogenesis. Herein, ultrasmall nanoscale coordination polymers (NCPs) coordinated by ferric ions and natural product curcumin (Cur) were exploited, showing efficient neuroprotection by scavenging excessive radicals and suppressing neuroinflammation. In a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse PD model, such ultrasmall Fe-Cur NCPs with prolonged blood circulation and BBB traversing capability could effectively alleviate oxidative stress, mitochondrial dysfunction, and inflammatory condition in the midbrain and striatum to reduce PD symptoms. Thus, this study puts forth a unique type of therapeutics-based NCPs that could be used for safe and efficient treatment of PD with potential in clinical translation.

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Section: Analytical Techniques For Probing the Protein Coronamentioning

confidence: 99%

Chemical and Biophysical Signatures of the Protein Corona in Nanomedicine

et al. 2022

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An inconvenient hurdle in the practice of nanomedicine is the protein corona, a spontaneous collection of biomolecular species by nanoparticles in living systems. The protein corona is dynamic in composition and may entail improved water suspendability and compromised delivery and targeting to the nanoparticles. How much of this nonspecific protein ensemble is determined by the chemistry of the nanoparticle core and its surface functionalization, and how much of this entity is dictated by the biological environments that vary spatiotemporally in vivo? How do we “live with” and exploit the protein corona without significantly sacrificing the efficacy of nanomedicines in diagnosing and curing human diseases? This article discusses the chemical and biophysical signatures of the protein corona and ponders challenges ahead for the field of nanomedicine.

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Tracing the In Vivo Fate of Nanoparticles with a “Non-Self” Biological Identity

Cited by 13 publications

References 60 publications

Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins

Effects of Protein Source on Liposome Uptake by Cells: Corona Composition and Impact of the Excess Free Proteins

Ultrasmall Coordination Polymers for Alleviating ROS-Mediated Inflammatory and Realizing Neuroprotection against Parkinson’s Disease

Chemical and Biophysical Signatures of the Protein Corona in Nanomedicine

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