Tracheoplasty with cartilage-engineered esophagus environments

Komura, Makoto; Komura, Hiroko; Otani, Yushi; Suzuki, Keisuke; Satake, Ryosuke; Kodaka, Tetsuro; Terawaki, Kan; Yonekawa, Hironobu; Ikebukuro, Kenichi; Hoshi, Kazuto; Takato, Tsuyoshi; Tabata, Yasuhiko; Komuro, Hiroaki; Iwanaka, Tadashi

doi:10.1016/j.jpedsurg.2014.07.005

Cited by 10 publications

(11 citation statements)

References 30 publications

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“…[45] utilized GA in different concentrations as a crosslinker with no report of cytotoxicity. In our study, no destructive effects on chondrocytes were observed to be caused by the concentration of 0.8 wt% GA similar to the data reported by Komura and coworkers[46] based on crosslinking gelatin sponges with 0.16 wt% GA. GA concentration affects mechanical characteristics, swelling ratio, and degradation, which are significant features of an optimized scaffold. As it can be seen in the FTIR spectra (Fig.…”

Section: Discussionsupporting

confidence: 92%

Fabrication of Chitosan Silk-based Tracheal Scaffold Using Freeze-Casting Method

Nematollahi

Tafazzoli-Shadpour

Zamanian

et al. 2017

IBJ

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Background:Since the treatments of long tracheal lesions are associated with some limitations, tissue engineered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering.Methods:Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) and glutaraldehyde (GA) concentrations (0, 0.4, and 0.8 wt%). Samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments.Results:The pore sizes were highly influenced by the freezing rate and varied from 135.3×372.1 to 37.8×83.4 µm. Swelling and biodegradability behaviors were more affected by GA rather than freezing rate. Tensile strength raised from 120 kPa to 350 kPa by an increment of freezing rate and GA concentration. In addition, marked stiffening was demonstrated by increasing elastic modulus from 1.5 MPa to 12.2 MPa. Samples having 1 and 2°C/min of freezing rate and 0.8 wt% GA concentration made a non-toxic, porous structure with tensile strength and elastic modulus in the range of human trachea, facilitating the chondrocyte proliferation. The results of 21-day cell culture indicated that glycosaminoglycans content was significantly higher for the rate of 2°C/min (12.04 µg/min) rather than the other (9.6 µg/min).Conclusion:A homogenous porous structure was created by freeze drying. This allows the fabrication of a chitosan silk scaffold cross-linked by GA for cartilage tissue regeneration with application in tracheal regeneration.

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Section: Discussionsupporting

confidence: 92%

Fabrication of Chitosan Silk-based Tracheal Scaffold Using Freeze-Casting Method

Nematollahi

Tafazzoli-Shadpour

Zamanian

et al. 2017

IBJ

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“…In pre-clinical studies, the most common site for harvesting differentiated chondrocytes is auricular elastic cartilage [ 60 – 67 ]. Despite the hyaline nature of tracheal rings, there is some evidence that the origin of chondrocyte does not affect the success of tracheal hyaline regeneration in pre-clinical small patch tracheoplasties [ 67 – 69 ].…”

Section: Autologous Cell Choices For Tracheal Regenerationmentioning

confidence: 99%

“…Outcome measures in studies involving chondrocyte seeding have included the persistence of chondrocytes within scaffolds for up to 14 weeks post-operatively [ 60 , 69 , 76 – 80 ] and the detection of appropriate extracellular matrix protein deposition, specifically collagen, elastin, and GAGs [ 62 , 81 ]. Areas of “neocartilage” formation following chondrocyte seeding have been reported in several small pre-clinical studies of synthetic [ 60 , 68 , 78 , 81 ] and decellularized [ 70 ] grafts, though few of these reports include unseeded control groups, making data interpretation somewhat difficult. Moreover, these reports have not included comments on the functionality of the new cartilage in contributing to the mechanical stability of grafts, despite the particular relevance of this in studies where scaffolds are manufactured from biodegradable material.…”

Section: Autologous Cell Choices For Tracheal Regenerationmentioning

confidence: 99%

“…Without deep biopsy of the implanted grafts, it is not possible to comment on the persistence or eventual fate of the seeded MSCs or chondrocytes nor the role these cells played in the overall graft success in such patients. Despite pre-clinical studies [ 60 , 62 , 63 , 65 , 67 , 71 , 76 , 78 , 80 , 81 , 83 – 86 ], to our knowledge, there have been no clinical reports of the use of harvested differentiated chondrocytes in tracheal tissue engineering.…”

Section: Autologous Cell Choices For Tracheal Regenerationmentioning

confidence: 99%

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Autologous Cell Seeding in Tracheal Tissue Engineering

et al. 2017

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Purpose of ReviewThere is no consensus on the best technology to be employed for tracheal replacement. One particularly promising approach is based upon tissue engineering and involves applying autologous cells to transplantable scaffolds. Here, we present the reported pre-clinical and clinical data exploring the various options for achieving such seeding.Recent FindingsVarious cell combinations, delivery strategies, and outcome measures are described. Mesenchymal stem cells (MSCs) are the most widely employed cell type in tracheal bioengineering. Airway epithelial cell luminal seeding is also widely employed, alone or in combination with other cell types. Combinations have thus far shown the greatest promise. Chondrocytes may improve mechanical outcomes in pre-clinical models, but have not been clinically tested. Rapid or pre-vascularization of scaffolds is an important consideration. Overall, there are few published objective measures of post-seeding cell viability, survival, or overall efficacy.SummaryThere is no clear consensus on the optimal cell-scaffold combination and mechanisms for seeding. Systematic in vivo work is required to assess differences between tracheal grafts seeded with combinations of clinically deliverable cell types using objective outcome measures, including those for functionality and host immune response.Electronic supplementary materialThe online version of this article (10.1007/s40778-017-0108-2) contains supplementary material, which is available to authorized users.

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“…Thus, the use of personalized 3DP for TM remains to be defined. Potential therapies recently studied in animal models might include tissue-engineering techniques with collagenous connective tissue membranes (bio sheets) as a potential tracheal substitute material (21,22). These will have to be validated in humans prior to implementation in practice.…”

mentioning

confidence: 99%