Tracer Study of Pollen-Tube Growth in Cross-Sterile Maize

House, L. R.; Nelson, Oliver E.

doi:10.1093/oxfordjournals.jhered.a106755

Cited by 29 publications

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“…In our experiments, pollen was a non-limiting factor since pollen viability was controlled before pollination, and the pollination technique was the same for each spikelet. We found rates of in vitro pollen-tube growth in the silks similar to those previously reported in vivo; these range from 2 to 12 mm/h for silk lengths from 3 to 25 cm and for various genotypes (Randolph 1936;House and Nelson 1958;Kumar and Sarkar 1980). Furthermore, the length of the silks can be defined precisely using the in vitro pollination fertilization technique, and this leads to a more precise definition of the pollen-tube growth rate.…”

Section: Discussionsupporting

confidence: 85%

“…An autoradiographic labelling technique (House and Nelson 1958;Kumar and Sarkar 1980) was used to follow the pathway of the pollen tubes in silks after in vitro pollination. On the first day of anthesis tassels were removed from the plant, and the cut stalk was immersed in distilled water containing radioactive 32p in the form of phosphoric acid (activity 500 gCi/5 ml).…”

Section: P_labeliing Of Pollen Tube Growthmentioning

confidence: 99%

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In vitro pollination as a model for studying fertilization in maize (Zea mays L.)

Dupuis

Dumas

1989

Sexual Plant Reprod

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In vitro pollination was conducted using excised segments of maize female spikelets to determine the effects of age and silk length on fertilization efficiency and developmental pattern. Ovary development after 15 days resulted in: (1)normal kernels, (2)abnormal kernels and (3)enlarged ovaries; the percentages of each class varied with age. Evidence of double fertilization was observed in both normal and abnormal kernels. In vitro fertilization was traced using silk excision and autoradiography with 32p-radiolabelled pollen and occurred between 4 and 7 h after the pollination of 4.5-cm-long silks. This study supports the validity of the in vitro pollination method for studying fertilization and emphasizes the importance of using a developmentally sensitive index (silk length) for establishing female developmental stage.

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Section: Discussionsupporting

confidence: 85%

Section: P_labeliing Of Pollen Tube Growthmentioning

confidence: 99%

In vitro pollination as a model for studying fertilization in maize (Zea mays L.)

Dupuis

Dumas

1989

Sexual Plant Reprod

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“…In roses, the few authors who have used in vitro germination assays as a viability criterion have only considered germination rates (Visser et al 1977;Pearson and Harney 1984). The use of only this criterion for pollen quality evaluation has been criticized by Knox et al (1986) as methods that enable in situ pollen growth evaluation (see House and Nelson 1958;Kumar and Sarkar 1980) have cleary established that this last factor is better correlated to the achievement of gametic union; Dumas et al (1988) consider it to be "the only significant measurement of pollen function".…”

Section: Introductionmentioning

confidence: 99%

Influence of season on rose pollen quality

Gudin¹,

Aréne²,

Bulard³

1991

Sexual Plant Reprod

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In vitro tests of pollen germination were carried out at different periods during an annual cycle in order to study environmental influence on the quality of Rosa hybrida L. pollen during its maturation process. This quality was evaluated by taking into account the rate of germination as well as the average length of emitted pollen tubes. In addition, during an annual hybridization period, a few pollinations were carried out in vivo with pollen of the same origin in order to study the evolution of the fertilization results, as attested by number of achenes per resulting hips. During the period covered by the experiments, the evolution of the pollen quality detected in vitro can be related to that of seed setting success. Of the two criteria used in vitro to evaluate pollen quality, the factor most liable to influence in vivo fertilization success seems to be the average length of emitted pollen tubes.

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“…With a more appropriate staining technique, the pollen-tube growth inside the silks can be observed. 32 The use of radioactive P tracer technique for the study of pollen-tube growth in live silks has been suggested by House and Nelson (1958). However, the necessary equipment required for this technique is not available at this time.…”

Section: E Preliminary Studies On Pollen Germination and Pollen-tubementioning

confidence: 99%

Nonreciprocal partial cross-incompatibility in maize

Sukhapinda¹,

Petérson²

1983

Can. J. Genet. Cytol.

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A case of cross-incompatibility among crosses in our genetics nursery was first observed in 1975. These crosses though involving controlling elements in maize (Zea mays L.) do not necessarily implicate these elements. When the derivative genotypes from the genetic stocks were crossed by specific male parents, ears with reduced seed set (RSS) were produced. In the reciprocal cross where these same genetic stocks were used as the male parent, ears with normal seed set (NSS) were obtained. The RSS effect is a heritable character caused by an incompatibility interaction between the female tissue and a specific pollen type. The genetic control of the incompatibility interaction has been hypothesized to be influenced by a cytoplasmic–chromosomal interaction in the female and a chromosomal factor in the pollen. The site of the incompatibility interaction is not on the silk surface because the incompatible pollen germinated and developed normally on the silk surface. Thus, the incompatibility interaction can occur either inside the silk or the ovule.

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Tracer Study of Pollen-Tube Growth in Cross-Sterile Maize

Cited by 29 publications

References 0 publications

In vitro pollination as a model for studying fertilization in maize (Zea mays L.)

In vitro pollination as a model for studying fertilization in maize (Zea mays L.)

Influence of season on rose pollen quality

Nonreciprocal partial cross-incompatibility in maize

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