“…In order to mitigate interferences, CFS samples are usually diluted before their analysis via ICP-MS. 4,8,[20][21][22][23][24][25][26][27][28][29][30][31][32] The dilution factor goes from 1:4 to 1:50, depending on the element to be quantified. According to some studies, a CFS digestion should be performed in order to decompose the organic CSF concomitants.…”
Section: Resultsmentioning
confidence: 99%
“…8,12 Trace elements have been determined in CSF through voltammetry 13 , atomic absorption spectrometry (AAS), 9,14-19 inductively coupled plasma optical emission spectrometry (ICP-OES) [20][21][22][23][24] and inductively coupled plasma mass spectrometry (ICP-MS). 5,9, [21][22][23][24][25][26][27][28][29][30][31][32][33] The last technique is the most suitable to perform this kind of analysis, due to its multi-element capability, high sensitivity and wide dynamic range.…”
Section: -10mentioning
confidence: 99%
“…35 Fortunately, these unwanted phenomena can be minimized or even removed by using a reaction and/or collision cell in a quadrupole based ICP-MS instrument. 24,28,29,36 Alternatively, interferences are overcome by sample dilution, which degrades the sensitivity. 20 In any case, it should be considered that the total CSF volume in adults is estimated to be about 100-150 mL.…”
A total consumption low sample introduction system has been applied for the first time to the multielemental analysis of non-diluted cerebrospinal fluids (CSF) by means of inductively coupled plasma mass spectrometry (ICP-MS). A 2.5 µL sample volume has been injected into an air carrier stream in agreement with the air-segmented injection principle. The sample plug has been turned out into an aerosol by means of a high efficiency nebulizer (HEN) and further introduced into the so-called high temperature torch integrated sample introduction system (hTISIS). A transient signal has been thus obtained. For spiked CSF real samples it has been verified that the higher the temperature, the greater the sensitivity. Under optimized conditions, the hTISIS provides peak areas around four times higher than those provided by the spectrometer default device (i.e., a double pass spray chamber). Additional advantages incorporated by the former system include limits of detection up to 6 times lower and narrower peaks as compared to those reported for the double pass spray chamber.Furthermore, the use of the hTISIS is not detrimental from the point of view of neither oxide production nor doubly charged ion generation. Regarding the extent of nonspectral interferences caused by the CSF matrix, it has been verified that, with the hTISIS, recoveries for spiked real samples were close to 100% for a set of 14 different elements (V, Cr, Mn, Co, Ni, Cu, As, Se, Mo, Cd, Sb, Ba, Tl and Pb). Meanwhile, in segmented flow injection mode, the reference system provided recoveries from 200 to 500%, depending on the element and the sample, thus demonstrating the occurrence of matrix effects.
“…In order to mitigate interferences, CFS samples are usually diluted before their analysis via ICP-MS. 4,8,[20][21][22][23][24][25][26][27][28][29][30][31][32] The dilution factor goes from 1:4 to 1:50, depending on the element to be quantified. According to some studies, a CFS digestion should be performed in order to decompose the organic CSF concomitants.…”
Section: Resultsmentioning
confidence: 99%
“…8,12 Trace elements have been determined in CSF through voltammetry 13 , atomic absorption spectrometry (AAS), 9,14-19 inductively coupled plasma optical emission spectrometry (ICP-OES) [20][21][22][23][24] and inductively coupled plasma mass spectrometry (ICP-MS). 5,9, [21][22][23][24][25][26][27][28][29][30][31][32][33] The last technique is the most suitable to perform this kind of analysis, due to its multi-element capability, high sensitivity and wide dynamic range.…”
Section: -10mentioning
confidence: 99%
“…35 Fortunately, these unwanted phenomena can be minimized or even removed by using a reaction and/or collision cell in a quadrupole based ICP-MS instrument. 24,28,29,36 Alternatively, interferences are overcome by sample dilution, which degrades the sensitivity. 20 In any case, it should be considered that the total CSF volume in adults is estimated to be about 100-150 mL.…”
A total consumption low sample introduction system has been applied for the first time to the multielemental analysis of non-diluted cerebrospinal fluids (CSF) by means of inductively coupled plasma mass spectrometry (ICP-MS). A 2.5 µL sample volume has been injected into an air carrier stream in agreement with the air-segmented injection principle. The sample plug has been turned out into an aerosol by means of a high efficiency nebulizer (HEN) and further introduced into the so-called high temperature torch integrated sample introduction system (hTISIS). A transient signal has been thus obtained. For spiked CSF real samples it has been verified that the higher the temperature, the greater the sensitivity. Under optimized conditions, the hTISIS provides peak areas around four times higher than those provided by the spectrometer default device (i.e., a double pass spray chamber). Additional advantages incorporated by the former system include limits of detection up to 6 times lower and narrower peaks as compared to those reported for the double pass spray chamber.Furthermore, the use of the hTISIS is not detrimental from the point of view of neither oxide production nor doubly charged ion generation. Regarding the extent of nonspectral interferences caused by the CSF matrix, it has been verified that, with the hTISIS, recoveries for spiked real samples were close to 100% for a set of 14 different elements (V, Cr, Mn, Co, Ni, Cu, As, Se, Mo, Cd, Sb, Ba, Tl and Pb). Meanwhile, in segmented flow injection mode, the reference system provided recoveries from 200 to 500%, depending on the element and the sample, thus demonstrating the occurrence of matrix effects.
“…It is reported that zinc concentration in CSF is in the range 150-380 nM. [32][33][34] If it is the same as zinc concentration in the brain extracellular fluid, a large portion of zinc is not free ion in the brain extracellular fluid under the basal (static) condition. At zincergic synapses such as Shaffer collateral and mossy fiber synapses, extracellular Zn 2+ levels are dynamically modified by the degree of activity-dependent Zn 2+ release, which is required for learning and memory via synaptic plasticity as described below.…”
The basal levels of extracellular Zn 2 are in the range of low nanomolar concentrations in the hippocampus and perhaps increase age-dependently. Extracellular Zn 2 dynamics is critical for cognitive activity and excess influx of extracellular Zn 2 into hippocampal neurons is a known cause of cognitive decline. The dentate gyrus is vulnerable to aging in the hippocampus and affected in the early stage of Alzheimer's disease (AD). The reasons remain unclear. Neurogenesis-related apoptosis may induce non-specific neuronal depolarization by efflux of intracellular K in the dentate gyrus and be markedly increased along with aging. Extracellular Zn 2 influx into dentate granule cells via high K -induced perforant pathway excitation leads to cognitive decline. Modified extracellular Zn 2 dynamics in the dentate gyrus of aged rats is linked with vulnerability to cognitive decline. Amyloid-β 1-42 (Aβ 1-42 ) is a causative candidate for AD pathogenesis. When Aβ 1-42 concentration reaches picomolar in the extracellular compartment in the dentate gyrus, Zn-Aβ 1-42 is formed in the extracellular compartment and rapidly taken up into dentate granule cells, followed by Aβ 1-42 -induced cognitive decline that is due to Zn 2 released from Aβ 1-42 , suggesting that dentate granule cells are sensitive to extracellular Zn 2 -dependent Aβ 1-42 toxicity. This paper deals with proposed vulnerability of the dentate gyrus to aging and Aβ 1-42 neurotoxicity.
“…Likewise reported Mg levels from Norway and China were lower than the values in this study (46.23µg/mL). The differences may be due to difference in geographical locations, dietary habits, genetic variations, socioeconomic status, and traditions among others (Eyad et al, 2006, Gellein et al, 2008, Moreno et al, 1999, Mumtaz, 1999, Raja et al 2009, Xilei et al 1988.…”
Section: Fig 4 Mean Metals Concentration (µG/ml) In Blood Of Pregnantmentioning
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