Trace element analysis by means of particle induced X-ray emission with triggered beam pulsing

Koenig, W.; Richter, F.-W.; Steiner, Urs; Stock, R.; Thielmann, R.; Wätjen, U.

doi:10.1016/0029-554x(77)90832-1

Cited by 38 publications

(2 citation statements)

References 2 publications

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“…To decrease the number of low energy events registered in the X-ray detector, 155 pm of mylar was placed in front of the detector window. To diminish the effect of spurious peaks in the X-ray spectra, caused by pulse pileup, a triggered beam pulsing system was employed [ 19], The setup has been described in detail earlier [16).…”

Section: Pixe Analysismentioning

confidence: 99%

Zinc in the Anterior Pituitary of Rat: A Histochemical and Analytical Work

Thorlacius‐Ussing¹

1987

Neuroendocrinology

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Recent studies have suggested zinc to be a possible modulator of hormone secretion from the anterior pituitary. The three aims of the present study were: (1) to estimate the total amount of zinc in the gland by instrumental analysis; (2) to visualize the zinc at light and electron microscopical levels, and (3) to estimate the amount of zinc that can be visualized by histochemical techniques. PIXE measurement showed the total amount of zinc to be 74 ng/mg dry weight in males and 100 ng/mg dry weight in females, which is a highly significant difference. Histochemically, the zinc was shown by a modified Timm method and the selenium method to be localized to the secretory granules, and to a smaller extent to the Golgi apparatus of the somatotrophs, corticotrophs and thyrotrophs. Chelation of tissue zinc by intravital dithizone treatment effectively blocked subsequent selenium and Timm staining in the secretory granules, and PIXE assays of the chelated metal (extracted into CCU) showed it constitued less than 5% of the total zinc in the tissue. It is concluded from the study that zinc is present in the anterior pituitary of rats in rather high amounts and that loosely bound zinc, which is suggested to exert a biological effect by itself, can be located to the parts of the cells responsible for production, storing and release of hormones.

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Section: Pixe Analysismentioning

confidence: 99%

Zinc in the Anterior Pituitary of Rat: A Histochemical and Analytical Work

Thorlacius‐Ussing¹

1987

Neuroendocrinology

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“…The pulsed beam technique (Koenig et al, 1977) removes the beam after each detector pulse to permit complete recovery of the pulse proces sor and greatly alleviates the above problems. The pulsed beam technique (Koenig et al, 1977) removes the beam after each detector pulse to permit complete recovery of the pulse proces sor and greatly alleviates the above problems.…”

Section: Fig 3 X-ray Spectrum From a 30 μ\ Blood Serum Sample Excitmentioning

confidence: 99%