Trace DNA collection—Performance of minitape and three different swabs

Evaluation of Low Trace DNA Recovery Techniques from Ridged Surfaces

“…There is also a study by Hansson et al investigating DNA recovery techniques from clothing [6] which also considered flocked swabs as well as tape lifts. This study also indicated that tape lifts recovered more DNA from clothing than flocked swabs; thus supporting the findings of this study, albeit on a different surface type.…”

“…This causes problems during the extraction phase as the swab itself is protecting the DNA from the detergent and proteinases. The flocked swabs overcome this by only having absorbent surfaces at the tip of each bristle, thus minimising any wicking effect and increasing the surface area for exposure to the proteinases [6].…”

Avoiding Errors and Pitfalls in Evidence Sampling for Forensic Genetics

“…Co-extraction of these swabs to enhance overall retrieval of DNA is recommended (Castella and Mangin, 2008;Pang and Cheung, 2007). The moistening agent can be sterile water, 0, 01% sodium dodecyl sulphate (Wickenheiser, 2002) or isopropanol (Hansson et al, 2009). The quantities of cellules retrieved depend also of the physical characteristics of the surface (Wickenheiser, 2002) and the use of different moistening agents for different surfaces may facilitate collection.…”

“…The quantities of cellules retrieved depend also of the physical characteristics of the surface (Wickenheiser, 2002) and the use of different moistening agents for different surfaces may facilitate collection. The quality of the swabs is also important, the quality should be DNA-free; cotton swabs are the most frequently used but other types such as foam may also be considered (Wickenheiser, 2002;Hansson et al, 2009;57, 111, 112). It has been shown that the yield of DNA from moist or frozen swabs are higher that from dried swabs.…”

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

“…Various types of swabs and tape are used [84,[86][87][88][89]. Retrieval of DNA depends on the sampling force-e.g., the pressure and actuation of the swab and contact intensity of adhesives-at the targeted area of devices and body, but may only partially pick up the available sample.…”