Trace determination of tetrahydrocannabinol (THC) in cosmetic products by stir bar sorptive dispersive microextraction followed by liquid chromatography-tandem mass spectrometry

Azorín, Cristian; Benedé, Juan L.; Chisvert, Alberto; Salvador, Amparo

doi:10.1016/j.talanta.2022.123934

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…The Japanese Minister of Health, Labour and Welfare investigated these distributed CBD oil products and THC was detected (undisclosed quantitative values). Thus, we validate the evidence of the adequacy as follows: (1) an analytical strategy based on chromatographic technique is needed for an accurate and simple procedure (Tsujikawa et al, 2022, and Supporting Information); (2) the limit of quantification (LOQ) value should be indicated for high CBD contents and trace amounts of THC/other cannabinoids in oil products (Azorín et al, 2023); and (3) CBD stability should be investigated for the convertible THC in oil products with various additives at high temperatures (Takashina et al, 2022, and Supporting Information). Moreover, we have improved the liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay by adding Δ 8 ‐THC and tetrahydrocannabivarin (THCV), which is a pharmacological isomer warned to be harmful (Abioye et al, 2020, and Supporting Information).…”

Section: Introductionmentioning

confidence: 67%

Quality test of cannabidiol profiling in CBD oil products and evaluation of residual THC at high temperature based on liquid chromatography coupled with tandem mass spectrometry

Takashina

Minamoto

Nunome

et al. 2023

Biomedical Chromatography

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Cannabidiol (CBD) oil products are available in Japan as cosmetics, fragrances, food and items. Herein, quality testing of cannabinoid profiling in CBD oil products and the evaluation of possible residual tetrahydrocannabinol (THC) in these products using liquid chromatography–tandem mass spectrometry (LC–MS/MS) was conducted. A simple, sensitive, and selective LC–MS/MS assay (electrospray positive ion mode) was employed for the simultaneous quantification of eight cannabinoids. This quantification with three different oil samples showed that accuracy rates ranged from 87.7 to 106.9% (RSD > 3.5%). Furthermore, the quantification limit of THC is 0.001 mg/g of CBD oil products for suitable levels lower than the regulatory value. Notably, this method was used to evaluate CBD oil products from the Japanese market. Additionally, we investigated the THC conversion in CBD oil products at a high temperature (70°C) which has a minor effect on CBD stability in oil products with additives. Herein, the developed LC–MS/MS assay is applied to monitor the quality of CBD, trace THC and other components in CBD oil products.

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Section: Introductionmentioning

confidence: 67%

Quality test of cannabidiol profiling in CBD oil products and evaluation of residual THC at high temperature based on liquid chromatography coupled with tandem mass spectrometry

Takashina

Minamoto

Nunome

et al. 2023

Biomedical Chromatography

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“…As known from previous works, , THC can partially be adsorbed on polypropylene (PP) materials (such as micropipette tips, syringes, microcentrifuge tubes, etc.) when prepared in aqueous solutions due to its high hydrophobicity.…”

Section: Resultsmentioning

confidence: 94%

Coupling Miniaturized Stir Bar Sorptive Dispersive Microextraction to Needle-Based Electrospray Ionization Emitters for Mass Spectrometry: Determination of Tetrahydrocannabinol in Human Saliva as a Proof of Concept

López-Juan,

Millán-Santiago,

Benedé

et al. 2024

Anal. Chem.

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Direct coupling of sample preparation with mass spectrometry (MS) can speed up analysis, enabling faster decision-making. In such combinations, where the analysis time is mainly defined by the extraction procedure, magnetic dispersive solid-phase extraction emerges as a relevant technique because of its rapid workflow. The dispersion and retrieval of the magnetic sorbent are typically uncoupled stages, thus reducing the potential simplicity. Stir bar sorptive dispersive microextraction (SBSDME) is a novel technique that integrates both stages into a single device. Its miniaturization (mSBSDME) makes it more portable and compatible with low-availability samples. This article reports the direct combination of mSBSDME and MS using a needle-based electrospray ionization (NESI) emitter as the interface. This combination is applied to determine tetrahydrocannabinol in saliva samples, a relevant societal problem if the global consumption rates of cannabis are considered. The coupling requires only the transference of the magnet (containing the sorbent and the isolated analyte) from the mSBSDME to the hub of a hypodermic needle, where the online elution occurs. The application of 5 kV on the needle forms an electrospray on its tip, transferring the ionized analyte to the MS inlet. The excellent performance of mSBSDME-NESI-MS/MS relies on the sensitivity (limits of detection as low as 2.25 ng mL–1), the precision (relative standard deviation lower than 15%), and the accuracy (relative recoveries ranged from 87 to 127%) obtained. According to the results, the mSBSDME-NESI-MS/MS technique promises faster and more efficient chemical analysis in MS-based applications.

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“…While methanol has been employed for the extraction of THC from hair, it may lead to contamination, ultimately affecting the sensitivity of assay by LC-MS. Thus, ltration steps are very important before injecting the sample into an LC-MS. 67 Also, the use of acetonitrile, aqueous NaOH solution and certain enzymes may also require a ltration step to have sufficiently pure samples for LCMS analysis. 68 The available data suggest that the concentration of cannabis in human hair is usually low, and the hair matrix contains various contaminants, which necessitates a preconcentration step to increase the yield of the analyte and eliminate the contaminants.…”

Section: Digestion Of Hair Samplesmentioning

confidence: 99%

“…While methanol has been employed for the extraction of THC from hair, it may lead to contamination, ultimately affecting the sensitivity of assay by LC-MS. Thus, filtration steps are very important before injecting the sample into an LC-MS. 67 Also, the use of acetonitrile, aqueous NaOH solution and certain enzymes may also require a filtration step to have sufficiently pure samples for LCMS analysis. 68…”

Section: Procedures For Sample Preparationsmentioning

confidence: 99%

Analytical techniques for screening of cannabis and derivatives from human hair specimens

Kale,

Chaturvedi,

Dandekar

et al. 2024

Anal. Methods

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Trace determination of tetrahydrocannabinol (THC) in cosmetic products by stir bar sorptive dispersive microextraction followed by liquid chromatography-tandem mass spectrometry

Cited by 7 publications

References 25 publications

Quality test of cannabidiol profiling in CBD oil products and evaluation of residual THC at high temperature based on liquid chromatography coupled with tandem mass spectrometry

Quality test of cannabidiol profiling in CBD oil products and evaluation of residual THC at high temperature based on liquid chromatography coupled with tandem mass spectrometry

Coupling Miniaturized Stir Bar Sorptive Dispersive Microextraction to Needle-Based Electrospray Ionization Emitters for Mass Spectrometry: Determination of Tetrahydrocannabinol in Human Saliva as a Proof of Concept

Analytical techniques for screening of cannabis and derivatives from human hair specimens

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