Trace contamination with dioxin-like chemicals: evaluation of bioassay-based TEQ determination for hazard assessment and regulatory responses

Overmeire, Ilse Van; Clark, George C.; Brown, D. J. A.; Chu, Michael D.; Cooke, W.M.; Denison, Michael S.; Baeyens, Willy; Srebrnik, S.; Goeyens, Léo

doi:10.1016/s1462-9011(01)00037-5

Cited by 34 publications

(16 citation statements)

References 68 publications

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“…Often different techniques are used (CALUX rat cells, CALUX mouse cells, GC-HRMS and analysis with or without separation of PCDD/Fs and dioxin-like PCBs), which makes clear interpretation and comparison of the results difficult. Also differences between clean up methods [19], the used dilution factor employed, different methods for quantification using CALUX standard curves [20] and the choice of TEF/REP values for quantification with GC-HRMS [21] all have an important influences on the quantification of the final result. Therefore, it is difficult to compare different studies with different protocols unless a correlation between the two methods has been established.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

Croes

Langenhove

Hond

et al. 2011

Talanta

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Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the throughput analysis of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in human serum with the new sensitive H1L7.5c1 mouse hepatoma cell line was optimized. Sample dilution factors of 5 and 2.4 were selected for routine analysis of respectively the PCDD/Fs and dl-PCBs. The validation studies showed that repeatability and within-lab reproducibility for the QC standard were within the in-house criteria. A long-term within-lab reproducibility of 25% for the PCDD/F fraction and 41% for the dl-PCB fraction for the analysis of pooled serum samples, expressed as pg BEQ/g fat, was determined. CALUX recoveries of the spiked procedural blanks were within the acceptable in-house limits of 80–120% for both fractions and the LOQ was 30.3 pg BEQ/g fat for the PCDD/Fs and 14.5 pg BEQ/g fat for the dl-PCBs. The GC-HRMS recovery of a C13-spiked pooled serum sample was between 60–90 % for all PCDD/F congeners and between 67–82 % for the non-ortho PCBs. An adequate separation between both fractions was found. The CALUX/GC-HRMS ratio for a pooled serum sample was respectively 2.0 and 1.4 for the PCDD/Fs and the dl-PCBs, indicating the presence of additional AhR active compounds. As expected, a correlation was found between human serum samples analyzed with both the new H1L7.5c1 cell line and the more established H1L6.1c3 cell line. The geometric mean CALUX-BEQ values, reported for the adolescents of the second Flemish Environment and Health Study (FLEHS II) recruited in 2009–2010, were 108 (95% CI: 101–114) pg CALUX-BEQ/g fat for the PCDD/Fs and 32.1 (30.1–34.2) pg CALUX-BEQ/g fat for the dioxin-like PCBs.

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Section: Conclusion and Discussionmentioning

confidence: 99%

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

Croes

Langenhove

Hond

et al. 2011

Talanta

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“…Toxic equivalents (TEQs) expressed as nanograms of TCDD per gram of sediment were calculated from EC50 values by use of the equieffective approach described by Villeneuve et al [28]. Toxic equivalents of TCDD (TEQs) were calculated using the toxicity equivalence factors determined for the CALUX bioassay [32].…”

Section: Discussionmentioning

confidence: 99%

Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling

Novák

Beníšek

Pachernı́k

et al. 2007

Enviro Toxic and Chemistry

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Abstract-Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, such as malformations in frogs or changes in metabolism of birds, could be related to disruption of the retinoid signaling pathway by exposure to organic contaminants. A new reporter gene assay has been established for evaluation of the modulation of retinoid signaling by individual chemicals or environmental samples. The bioassay is based on the pluripotent embryonic carcinoma cell line P19 stably transfected with the firefly luciferase gene under the control of a retinoic acid-responsive element (clone P19/ A15). The cell line was used to characterize the effects of individual chemicals and sediments extracts on retinoid signaling pathways. The extracts of sediments from the River Kymi, Finland, which contained polychlorinated dioxins and furans and polycyclic aromatic hydrocarbons (PAHs), significantly increased the potency of all-trans retinoic acid (ATRA), while no effect was observed with the extract of the sediment from reference locality. Considerable part of the effect was caused by the labile fraction of the sediment extracts. Also, several individual PAHs potentiated the effect of ATRA; on the other hand, 2,3,7,8-tetrachlorodibenzo-p-dioxin and several phthalates showed slightly inhibiting effect. These results suggest that PAHs could be able to modulate the retinoid signaling pathway and that they could be responsible for a part of the proretinoid activity observed in the sediment extracts. However, the effects of PAHs on the retinoic acid signaling pathways do not seem to be mediated directly by crosstalk with aryl hydrocarbon receptor.

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“…For this assay, AhR activity was evaluated using the chemical activated luciferase gene expression (CALUX) system, which was originally designed to detect DXNs [7]. The CALUX assay has been widely used for screening for dioxins in food and feed [8,9]. It is important to know the dioxin-like activity for various compounds in food to understand the possible influence of these compounds on our screening assay.…”

Section: Introductionmentioning

confidence: 99%

Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay

Amakura

Tsutsumi

Yoshimura

et al. 2016

Foods

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The purpose of this study was to examine whether a simple bioassay used for the detection of dioxins (DXNs) could be applied to detect trace amounts of harmful DXN-like substances in food products. To identify substances with possible DXN-like activity, we assessed the ability of various compounds in the environment to bind the aryl hydrocarbon receptor (AhR) that binds specifically to DXNs. The compounds tested included 19 polycyclic aromatic hydrocarbons (PAHs), 20 PAH derivatives (nitrated, halogenated, and aminated derivatives), 23 pesticides, six amino acids, and eight amino acid metabolites. The AhR binding activities (AhR activity) of these compounds were measured using the chemical activated luciferase gene expression (CALUX) reporter gene assay system. The majority of the PAHs exhibited marked AhR activity that increased in a concentration-dependent manner. Furthermore, there was a positive link between AhR activity and the number of aromatic rings in the PAH derivatives. Conversely, there appeared to be a negative correlation between AhR activity and the number of chlorine residues present on halogenated PAH derivatives. However, there was no correlation between AhR activity and the number and position of substituents among nitrated and aminated derivatives. Among the pesticides tested, the indole-type compounds carbendazim and thiabendazole showed high levels of activity. Similarly, the indole compound tryptamine was the only amino acid metabolite to induce AhR activity. The results are useful in understanding the identification and characterization of AhR ligands in the CALUX assay.

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Trace contamination with dioxin-like chemicals: evaluation of bioassay-based TEQ determination for hazard assessment and regulatory responses

Cited by 34 publications

References 68 publications

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish Environment and Health Study (FLEHS II)

Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling

Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay

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