Trace cobalt speciation in bacteria and at enzymic active sites using emission Mössbauer spectroscopy

Kamnev, Alexander A.; Antonyuk, L. P.; Smirnova, Victoria; Serebrennikova, Oksana B.; Куликов, Л. А.; Perfiliev, Yu. D.

doi:10.1007/s00216-001-1116-7

Cited by 21 publications

(3 citation statements)

References 26 publications

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“…The yield of the stabilized 57 Fe III components in samples 1 and 2, resulting from aftereffects of the 57 Co→ 57 Fe nuclear transformation15, 16 (22–26%; see Table I) is much lower than for dilute rapidly frozen (80 K) aqueous 57 CoCl 2 solutions,15 reflecting a firm binding of parent 57 Co II in the intersubunit spaces of the GS oligomer4 to the organic ligands with higher electron–donor properties.…”

Section: Resultsmentioning

confidence: 97%

Structural characterization of glutamine synthetase from Azospirillum brasilense

et al. 2004

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CD spectroscopic study of the secondary structure of partly adenylylated glutamine synthetase (GS) of the bacterium Azospirillum brasilense showed both the native and cation-free (EDTA-treated) enzyme to be highly structured (58 and 49% as alpha-helices, 10 and 20% as beta-structure, respectively). Mg(2+), Mn(2+), or Co(2+), when added to the native GS, had little effect on its CD spectrum, whereas their effects on the cation-free GS were more pronounced. Emission ((57)Co) Mössbauer spectroscopic (EMS) study of (57)Co(2+)-doped cation-free GS in frozen solution and in the dried state gave similar spectra and Mössbauer parameters for the corresponding spectral components, reflecting the ability of the Co(2+)-enzyme complex to retain its properties upon drying. The EMS data show that (a) A. brasilense GS has 2 cation-binding sites per active center and (b) one site has a higher affinity to Co(2+) than the other, in line with the data on other bacterial GSs.

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Section: Resultsmentioning

confidence: 97%

Structural characterization of glutamine synthetase from Azospirillum brasilense

et al. 2004

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“…This technology allowed elements in biological samples to be monitored at their physiological (trace) concentrations. It showed that Co-57(II)-activated glutamine synthetase had 2 different Co (II) forms at its active sites (Kamnev et al 2002a).…”

Section: Production Of Enzymes and Enzymatic Studiesmentioning

confidence: 99%

Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003)

Bashan¹,

Holguín²,

de‐Bashan³

2004

Can. J. Microbiol.

810

441

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This review presents a critical and comprehensive documentation and analysis of the developments in agricultural, environmental, molecular, and physiological studies related to Azospirillum cells, and to Azospirillum interactions with plants, based solely on information published between 1997 and 2003. It was designed as an update of previous reviews (Bashan and Levanony 1990; Bashan and Holguin 1997a), with a similar scope of interest. Apart from an update and critical analysis of the current knowledge, this review focuses on the central issues of Azospirillum research today, such as, (i) physiological and molecular studies as a general model for rhizosphere bacteria; (ii) co-inoculation with other microorganisms; (iii) hormonal studies and re-consideration of the nitrogen contribution by the bacteria under specific environmental conditions; (iv) proposed Azospirillum as a non-specific plant-growth-promoting bacterium; (v) re-introduction of the "Additive Hypothesis," which suggests involvement of multiple mechanisms employed by the bacteria to affect plant growth; (vi) comment on the less researched areas, such as inoculant and pesticide research; and (vii) proposes possible avenues for the exploitation of this bacterium in environmental areas other than agriculture.

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“…(Macías-Vázquez and Calvo-de-Anta, 2009)], could lead to a deficiency of this essential trace element with a broad range of biological functions (Kamnev et al, 2002), including key steps in protein synthesis (Williams and Fraústo da Silva, 2000), N 2 fixation by Azotobacter and nitrate reductase activity, crucial for microbial NO 3 --N assimilation (Nicholas et al, 1962). Conversely, in rocky areas, where soil pH (6.14) under natural vegetation was already below the optimum range, the acidification triggered by C. edulis invasion (down to pH 4.97) was strong enough to significantly decrease Cu, Mg and Zn availability.…”

Section: Discussionmentioning

confidence: 99%

Effects of Carpobrotus edulis invasion on main litter and soil characteristics in backdune and rocky coastal habitats with oceanic climate

Vieites-Blanco

González-Prieto

2018

Plant Soil

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Background and aims: Carpobrotus edulis invades coastal areas throughout the world, decreasing plant diversity and hampering restoration efforts by changing soil properties. Some of its effects on soils are known but there is a knowledge gap about the effects in rocky areas and micronutrients that we aimed to fill for dunes and rocky habitats with temperate-humid climate. Methods: We compared invaded vs non-invaded paired plots in two backdune and two rocky areas by measuring 18 variables in litter and 24 in soils (0-5 and 5-10 cm layers). Results: Invasion effects increased with the accumulated alien necromass, decreased with soil depth and are substrate-dependent: soil pH, Al, Fe and P increased in dunes, while these variables and Mg, Cu and Zn decreased in rocky sites. Carpobrotus necromass is richer in Mg and Ca and poorer in Al, Co, Cu, Fe, Ni and Zn than native necromass. Conclusions: Invader effects on soils are largely mediated by its necromass, which has contrasting characteristics with the autochtonous necromass. Carpobrotus edulis ability to discriminate against Al uptake, while favouring Mg and Ca uptake, and its lower requirement (or higher resorption) of key micronutrients (Co, Cu, Fe, Ni, Zn) than native vegetation could partly explain its invasiveness.

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Trace cobalt speciation in bacteria and at enzymic active sites using emission Mössbauer spectroscopy

Cited by 21 publications

References 26 publications

Structural characterization of glutamine synthetase from Azospirillum brasilense

Structural characterization of glutamine synthetase from Azospirillum brasilense

Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003)

Effects of Carpobrotus edulis invasion on main litter and soil characteristics in backdune and rocky coastal habitats with oceanic climate

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