The utility of a Markov chain Monte Carlo algorithm is, in large part, determined by the size of the spectral gap of the corresponding Markov operator. However, calculating (and even approximating) the spectral gaps of practical Monte Carlo Markov chains in statistics has proven to be an extremely difficult and often insurmountable task, especially when these chains move on continuous state spaces.In this paper, a method for accurate estimation of the spectral gap is developed for general state space Markov chains whose operators are non-negative and trace-class. The method is based on the fact that the second largest eigenvalue (and hence the spectral gap) of such operators can be bounded above and below by simple functions of the power sums of the eigenvalues. These power sums often have nice integral representations. A classical Monte Carlo method is proposed to estimate these integrals, and a simple sufficient condition for finite variance is provided. This leads to asymptotically valid confidence intervals for the second largest eigenvalue (and the spectral gap) of the Markov operator. In contrast with previously existing techniques, our method is not based on a near-stationary version of the Markov chain, which, paradoxically, cannot be obtained in a principled manner without bounds on the spectral gap. On the other hand, it can be quite expensive from a computational standpoint. The efficiency of the method is studied both theoretically and empirically. want to approximate the integralare the first m elements of a well-behaved Markov chain with stationary density π(·). Unlike classical Monte Carlo estimators,Ĵ m is not based on iid random elements. Indeed, the elements of the chain are typically neither identically distributed nor independent. Given var π f, the variance of f (·) under the stationary distribution, the accuracy ofĴ m is primarily determined by two factors: (i) the convergence rate of the Markov chain, and (ii) the correlation between the f (Φ k )s when the chain is stationary. These two factors are related, and can be analyzed jointly under an operator theoretic framework.The starting point of the operator theoretic approach is the Hilbert space of functions that are square integrable with respect to the target pdf, π(·). The Markov transition function that gives rise to Φ = {Φ k } ∞ k=0 defines a linear (Markov) operator on this Hilbert space. (Formal definitions are given in Section 2.) If Φ is reversible, then it is geometrically ergodic if and only if the corresponding Markov operator admits a positive spectral gap (Roberts and Rosenthal , 1997;Kontoyiannis and Meyn, 2012). The gap, which is a real number in (0, 1], plays a fundamental role in determining the mixing properties of the Markov chain, with larger values corresponding to better performance. For instance, suppose Φ 0 has pdf π 0 (·) such that dπ 0 /dπ is in the Hilbert space, and let d(Φ k ; π) denote the total variation distance between the distribution of Φ k and the chain's stationary distribution. Then, if δ denotes the s...