Trace Analysis of Methanol in Rat Serum by Headspace High Resolution Gas Chromatography/Selected Ion Monitoring

Abstract

A high resolution headspace gas chromatography/selected ion monitoring analytical method has been developed for the quantitation of methanol in body fluids. The addition of tetradeuterated methanol as internal standard gives this method high accuracy and reproducibility. The high sensitivity of the mass spectrometer permits analysis of methanol in aqueous solutions at concentrations down to 0.012 mg/l. The analytical procedure described was used to measure serum methanol in rats given 5 mg/kg of this alcohol orally. The method proved suitable for analysis of endogenous levels of methanol and of variations close to endogenous levels.

A rapid and accurate method for determination of methanol in alcoholic beverage by direct injection capillary gas chromatography

“…They used packed column and had to distil large amount of sample (about 100 mL) to obtain enough methanol for analysis. Subsequent GC method to determine methanol was modified to reduce the needed sample volume and to increase sensitivity using combinations of various packing materials (Sims, 1976;Segura et al, 1985), converting to capillary column (Wilson et al, 1991;Pollack and Kawagoa, 1991) and using more sensitive detectors (Davoli et al, 1986). The AOAC qualified GC method to determine methanol suggests using packed column (AOAC, 1990).…”

“…The working period for packed column is also known to be comparatively shorter than with capillary column. However, notwithstanding the use of packed column or capillary column, many current methods for determination of methanol still suggest pre-treatment of samples with solid-phase extraction (Pereira et al, 1999), head-space equilibrium (Davoli et al, 1986;Cheung and Lin, 1987), solvent extraction (Pollack and Kawagoa, 1991) and/or distillation (Blanch et al, 1992). These pre-treatments are the most time-consuming steps and sources of frequent errors.…”

Determination of Methanol by 4-[N-Methyl,N-(1-naphthylmethyl)]-amino-4-oxo-butanoic Acid in Presence of 4-Dimethylaminopyridine and 1-Ethyl-3-(3-dimethylaminopropyl) Carbodiimide Hydrochloride as Catalysts

“…[1][2][3][4][5][6] Methanol is often used as a solvent for the extraction of plant components. A number of methods, including enzymatic, [13][14] colorimetric, [15] gas chromatographic (GC) [16][17][18][19][20] and HPLC [21][22][23][24] techniques, have been reported for the determination of methanol in various matrices. So it must be removed finally from plant extract medicine.…”