Trace analysis of carbonyl compounds by liquid chromatography–mass spectrometry after collection as 2,4-dinitrophenylhydrazine derivatives

Differential effects of quercetin on hippocampus-dependent learning and memory in mice fed with different diets related with oxidative stress

“…ROS production was expressed as relative light units (RLUs). Carbonyls of proteins were determined immunochemically as adducts of 2,4-dinitrophenylhydrazine method [24].…”

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

“…Since the development of a reliable commercial electron spray LC/MS, it has become the mainstream method for the analysis of RCC-DNPH adducts. For example, subppb sensitivity and good reproducibility were obtained for RCC-DNPH adducts, including formaldehyde, acetaldehyde, acrolein, and acetone, analyzed using LC/MS [113]. Disinfected water (from outdoor swimming pools after chlorination) was analyzed for aldehydes, including RCCs, as DNPH-adducts using HPLC/electronspray ionization (ESI)/tandem mass spectrometry (LC/MS/MS) without sample pre-concentration.…”

High‐performance liquid chromatography and capillary electrophoresis: Methodological challenges for the determination of biologically relevant low‐aliphatic aldehydes in human saliva

“…Carbonyl compounds, including the low-aliphatic aldehydes formaldehyde, acetaldehyde and acrolein, have been shown to be major air pollutants since they can be generated directly from incomplete fossil fuel combustion or indirectly by photochemical oxidation of volatile organic compounds emitted by various industrial sources [2,3]. Given the toxic potential of these molecules, sensitive techniques are needed to detect them even at low concentrations and indeed a series of reliable chromatographic (HPLC) and capillary electrophoretic (CE) approaches have already been developed for their exact quantification in air and other environmental samples [4][5][6][7][8][9][10] and in human exhaled breath condensate [11]. In addition, since the above-mentioned aldehydes are also major components of cigarette smoke, gas and liquid chromatographic techniques have been specifically designed for determining their amount in sidestream and mainstream cigarette smoke [12][13][14].…”