Trace analysis by injection-generated gradient ion-pair chromatography with micro-bore column high-performance liquid chromatography

Sˇlais, Karel; Krejcˇi, Milosˇ; Kourˇilová, D.

doi:10.1016/s0021-9673(01)83378-1

Journal of Chromatography A

1986

DOI: 10.1016/s0021-9673(01)83378-1

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Trace analysis by injection-generated gradient ion-pair chromatography with micro-bore column high-performance liquid chromatography

Karel Sˇlais

Milosˇ Krejcˇi

D. Kourˇilová

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Cited by 14 publications

References 23 publications

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The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of pharmaceuticals in biofluids

Plumb¹,

Dear²,

Mallett³

et al. 1999

Rapid Commun. Mass Spectrom.

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Fast gradient capillary high performance liquid chromatography (HPLC) coupled to a mass spectrometer has been successfully used for the analysis of pharmaceutical compounds from biological matrices, in the femtogram on column range. In the work reported in this paper, the use of capillary HPLC, on the 180-micron internal diameter scale, has shown a 30-fold improvement in detection limits when compared to conventional 2-mm scale chromatography. The use of fast gradient elution resulted in a generic methodology which gave excellent chromatographic reproducibility and column longevity. This technique has been used in conjunction with simple protein precipitation, with no deleterious effect on either the column life or the chromatographic performance. The use of capillary HPLC in bioanalysis has the potential to give a significant increase in assay sensitivity with the equipment currently in use. In this paper the authors also present a modification to the current PE-Sciex ion-spray source which allows excellent spray adjustment in three dimensional accessibility, which is important when working with low flow rates, as well as reducing the inherent system dead volume.

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The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of pharmaceuticals in biofluids

Plumb¹,

Dear²,

Mallett³

et al. 1999

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

Microcolumn liquid chromatography with sample induced internal pH gradient

Šlais

1991

J Microcolumn Separations

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Abstract. An internal pH gradient of a wide range of pH can be developed using the pumped mobile phase containing only a few [3][4][5][6] simple buffering salts which differ in pka values by about 1 pk, unit or less. The use of several types of surface ionizable groups allows the internal pH gradient to be effective not only for separation of ampholytes but also for separation of anions of both strong and weak acids. The internal pH gradient can be induced simultaneously with solute introduction into the separation column. For this new method, the term "sample induced internal gradient" (SIIG) of pH was suggested. The method is demonstrated by separation of model mixtures of UV detectable anions and ampholytes on a dynamically modified microcolumn.

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Comparison of injection-generated gradient and isocratic ion-pair chromatography on short microbore column in HPLC

1986

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Trace analysis by injection-generated gradient ion-pair chromatography with micro-bore column high-performance liquid chromatography

Cited by 14 publications

References 23 publications

The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of pharmaceuticals in biofluids

The application of fast gradient capillary liquid chromatography/mass spectrometry to the analysis of pharmaceuticals in biofluids

Microcolumn liquid chromatography with sample induced internal pH gradient

Comparison of injection-generated gradient and isocratic ion-pair chromatography on short microbore column in HPLC

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