Trabecular thickness measurement in cancellous bones: postmortem rat studies with the zoom-in micro-tomography technique

Abstract

Using the cross-sectional images taken with the zoom-in micro-tomography technique, we measured trabecular thicknesses of femur bones in postmortem rats. Since the zoom-in micro-tomography technique is capable of high resolution imaging of a small local region inside a large subject, we were able to measure the trabecular thickness without extracting bone samples from the rats. For the zoom-in micro-tomography, we used a micro-tomography system consisting of a micro-focus x-ray source, a 1248 x 1248 flat-panel x-ray detector and a precision scan mechanism. To compensate for the limited spatial resolution in the zoom-in micro-tomography images, we used the fuzzy distance transform for the calculation of the trabecular thickness. To validate the trabecular thickness measurement with the zoom-in micro-tomography images, we compared the measurement results with those obtained from the conventional micro-tomography images of the extracted bone samples. The difference between the two types of measurement results was less than 2.5%.

Determination of proline–hydroxyproline dipeptide in rat urine by high-performance anion-exchange chromatography coupled with pulsed amperometric detection

“…A calibration curve was constructed using six different concentrations (1,5,10,25,50, and 100 M) of Pro, Hyp, and PHP, and excellent linearity was obtained. The equations and correlation coefficients (r 2 ) of the calibration curves were as follows: Pro, y = 0.0294x + 0.0033, r 2 = 0.9999; Hyp, y = 0.0418x + 0.1071, r 2 = 0.9991; and PHP, y = 0.0113x + 0.0185, r 2 = 0.9990.…”

“…The induction of osteoporosis was confirmed 22 weeks after ovariectomy based on two-dimensional imaging of the trabecular bone of the left femoral neck. The images were obtained using micro-computed tomography (Micro-CT) according to a previously published method [10]. Mean trabecular thickness was used as the bone morphometric parameter.…”

Royal Jelly Increases Collagen Production in Rat Skin After Ovariectomy

“…Three-dimensional tomographic images were taken by micro-CT according to the previously published method. 32 Rats were anesthetized (1.5% isofluorane, 70% N 2 O, and 30% O 2 gas) and maintained on anesthetic gases for the duration of each measurement. The 3D images (512 · 512 · 512) were reconstructed from 450 projection data and the isotropic pixel resolution was 25 lm.…”

Sparse-view CT imaging of trabecular bones: Comparison of image reconstruction methods

“…Particularly, trabecular bone imaging with a micro-CT is believed to be very useful for osteoporosis studies [5][6][7][8][9][10]. Longitudinal imaging of trabecular bone structure for a long time period through the life span of the animal subjects is often desired for evaluating the efficacy of new drugs or new therapeutic methods [11].…”