TQFL12, a novel synthetic derivative of TQ, inhibits triple‐negative breast cancer metastasis and invasion through activating AMPK/ACC pathway

Wei, Chunli; Zou, Hui; Xiao, Ting; Liu, Xiaoyan; Wang, Qian Qian; Cheng, Jingliang; Fu, Shangyi; Peng, Jiangzhou; Xie, Xin; Fu, Junjiang

doi:10.1111/jcmm.16945

Cited by 16 publications

(20 citation statements)

References 45 publications

(51 reference statements)

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“…3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to examine the cell viability ( 15 , 16 ). Cells were plated with 3,000~5,000 cells/well in a 96-well microplate for overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Matrigel was seeded on the upper chamber wells of CMI plates before invasion assay. Five micromoles or 10 μM of CD or DMSO was added after 10 h (cell growth) or 20 h (cell migration and invasion) of cell growth ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

“…Then, the primary antibodies were incubated at 4°C overnight. Next day, after being washed with TBST for three times and incubated in secondary antibodies for 1~2 h at room temperature, the membranes were detected after washing with TBST for the intensity of each band by an imaging scanner ( 16 ). Each experiment was repeated for three times.…”

Section: Methodsmentioning

confidence: 99%

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Cordycepin Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Regulating EMT-TFs SLUG, TWIST1, SNAIL1, and ZEB1

Wei

Cheng

et al. 2022

Front. Oncol.

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Cancer metastasis is the most important cause of cancer-related death, and epithelial-to-mesenchymal transition (EMT) plays crucial roles in cancer metastasis. Cordycepin (CD) is highly enriched in the medicinally used Cordyceps mushroom. In this study, we conducted the antimetastatic activities of CD, specifically focusing on its regulatory effects on EMT-inducing transcription factors (EMT-TFs) in triple-negative breast cancer (TNBC). Our study showed CD to inhibit the growth, migration, and invasion of BT549 and 4T1 cancer cell lines, by employing cell viability assay and real-time cell analyses. The protein levels of N-Cadherin and E-Cadherin, as well as their transcription factors TWIST1, SLUG, SNAIL1, and ZEB1 in BT549 and 4T1 cells, were estimated by Western blot assays. Results from dual-luciferase reporter assays demonstrated that CD is capable of inactivating the EMT signaling pathway by inhibiting TWIST1 and SLUG expression. Furthermore, in vivo studies with mice carrying cancer cell-derived allograft tumors showed the inhibitory effect of CD on cancer cell growth and metastasis. Furthermore, the additive/synergistic anti-metastasis effect of CD and thymoquinone (TQ), another natural product with promising anticancer roles, was demonstrated by combinational treatment. The results from this research indicate that CD would be a promising therapeutic molecule against TNBC by targeting EMT-TFs, possibly in SLUG, TWIST1, SNAIL1, and ZEB1.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cordycepin Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Regulating EMT-TFs SLUG, TWIST1, SNAIL1, and ZEB1

Wei

Cheng

et al. 2022

Front. Oncol.

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“…It is an energy-sensitive molecule and is activated under conditions of cellular stress, a common phenomenon in cancer cells. However, a few reports argue this activation may actually protect cancer cells [41]; nevertheless, there is ample evidence to suggest the therapeutic action of AMPK activation on cancer stem cells, particularly breast cancer stem cells [31,[42][43][44][45][46][47]. Paclitaxel, a well-known and commonly used drug for the treatment of breast and lung cancers, involves the activation of AMPK during its therapeutic pathway [48].…”

Section: Ampk Activation For the Treatment Of Breast Cancer Stem Cellsmentioning

confidence: 99%

Targeting Breast Cancer and Their Stem Cell Population through AMPK Activation: Novel Insights

Uprety

Abrahamse

2022

Cells

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Despite some significant advancements, breast cancer has become the most prevalent cancer in the world. One of the main reasons for failure in treatment and metastasis has been attributed to the presence of cancer initiating cells—cancer stem cells. Consequently, research is now being focussed on targeting cancer cells along with their stem cell population. Non-oncology drugs are gaining increasing attention for their potent anticancer activities. Metformin, a drug commonly used to treat type 2 diabetes, is the best example in this regard. It exerts its therapeutic action by activating 5′ adenosine monophosphate-activated protein kinase (AMPK). Activated AMPK subsequently phosphorylates and targets several cellular pathways involved in cell growth and proliferation and the maintenance of stem-like properties of cancer stem cells. Therefore, AMPK is emerging as a target of choice for developing effective anticancer drugs. Vanadium compounds are well-known PTP inhibitors and AMPK activators. They find extensive applications in treatment of diabetes and obesity via PTP1B inhibition and AMPK-mediated inhibition of adipogenesis. However, their role in targeting cancer stem cells has not been explored yet. This review is an attempt to establish the applications of insulin mimetic vanadium compounds for the treatment of breast cancer by AMPK activation and PTP1B inhibition pathways.

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“…TQFL12, a new derivative, is more effective compared to thymoquinone. TQFL12 caused shrinkage of tumor xenografts in mice orthotopically implanted with 4T1 cancer cells [ 104 ].…”

Section: Metastasis Inhibitory Role Of Thymoquinone: Animal Model Stu...mentioning

confidence: 99%

Anticancer and Anti-Metastatic Role of Thymoquinone: Regulation of Oncogenic Signaling Cascades by Thymoquinone

Farooqi

Attar

2022

IJMS

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Cancer is a life-threatening and multifaceted disease. Pioneering research works in the past three decades have mechanistically disentangled intertwined signaling networks which play contributory roles in carcinogenesis and metastasis. Phenomenal strides have been made in leveraging our scientific knowledge altogether to a new level of maturity. Rapidly accumulating wealth of information has underlined a myriad of transduction cascades which can be pharmaceutically exploited for cancer prevention/inhibition. Natural products serve as a treasure trove and compel interdisciplinary researchers to study the cancer chemopreventive roles of wide-ranging natural products in cell culture and preclinical studies. Experimental research related to thymoquinone has gradually gained momentum because of the extra-ordinary cancer chemopreventive multifunctionalities of thymoquinone. In this mini-review, we provide an overview of different cell signaling cascades reported to be regulated by thymoquinone for cancer chemoprevention. Essentially, thymoquinone efficacy has also been notably studied in animal models, which advocates for a rationale-based transition of thymoquinone from the pre-clinical pipeline to clinical trials.

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TQFL12, a novel synthetic derivative of TQ, inhibits triple‐negative breast cancer metastasis and invasion through activating AMPK/ACC pathway

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 16 publications

References 45 publications

Cordycepin Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Regulating EMT-TFs SLUG, TWIST1, SNAIL1, and ZEB1

Cordycepin Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Regulating EMT-TFs SLUG, TWIST1, SNAIL1, and ZEB1

Targeting Breast Cancer and Their Stem Cell Population through AMPK Activation: Novel Insights

Anticancer and Anti-Metastatic Role of Thymoquinone: Regulation of Oncogenic Signaling Cascades by Thymoquinone

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