TPV1, the first virus isolated from the hyperthermophilic genus <i>Thermococcus</i>

Gorlas, Aurore; Koonin, Eugene V.; Bienvenu, Nadège; Prieur, Daniël; Geslin, Claire

doi:10.1111/j.1462-2920.2011.02662.x

Cited by 69 publications

(90 citation statements)

References 68 publications

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“…For example, APSV1 encodes four other proteins with homologues in tailless spindle- shaped viruses, including the DnaA-like AAA ϩ ATPase believed to be involved in genome replication (45). Similarly, in addition to the MCP, PAV1 shares a three-gene cassette with A3-VLP (23,46), while with TPV1, it shares two other genes for putative minor structural proteins (3). Notably, our analysis has shown that all spindle-shaped viruses encode AAA ϩ ATPases.…”

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confidence: 99%

“…Over the years, a number of spindle-shaped viruses that could not be assigned to the existing taxa based on genome similarity have been isolated from phylogenetically distant archaeal lineages, including Thermococcales (Thermococcus prieurii virus 1 [TPV1] [3] and PAV1 [2,23] (22). We used this information to perform an in-depth genome analysis of all known unclassified spindle-shaped viruses.…”

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confidence: 99%

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Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea

Krupovìč

Quemin

Bamford

et al. 2014

J Virol

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Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horses" of the virosphere and segregate all spindle-shaped viruses into two distinct evolutionary lineages, corresponding to Bicaudaviridae and Fuselloviridae. Our results illuminate the utility of structure-based virus classification and bring additional order to the virosphere.

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confidence: 99%

mentioning

confidence: 99%

Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea

Krupovìč

Quemin

Bamford

et al. 2014

J Virol

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“…Functional studies proved to be highly challenging due to the lack of similarity between the protein sequences and structures of archaeal viruses and those from other viruses and cellular organisms (6)(7)(8)(9)(10). Among the morphotypes that are exclusively associated with archaea, spindle-shaped viruses are particularly widespread (11) and have been isolated from highly different environments, including deep-sea hydrothermal vents (12)(13)(14), hypersaline environments (15)(16)(17)(18), anoxic freshwaters (19), cold Antarctic lakes (20), terrestrial hot springs (21)(22)(23), and acidic mines (24).…”

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confidence: 99%

Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids

Quemin

Pietilä

Oksanen

et al. 2015

J Virol

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Geothermal and hypersaline environments are rich in virus-like particles, among which spindle-shaped morphotypes dominate. Currently, viruses with spindle-or lemon-shaped virions are exclusive to Archaea and belong to two distinct viral families. The larger of the two families, the Fuselloviridae, comprises tail-less, spindle-shaped viruses, which infect hosts from phylogenetically distant archaeal lineages. Sulfolobus spindle-shaped virus 1 (SSV1) is the best known member of the family and was one of the first hyperthermophilic archaeal viruses to be isolated. SSV1 is an attractive model for understanding virus-host interactions in Archaea; however, the constituents and architecture of SSV1 particles remain only partially characterized. Here, we have conducted an extensive biochemical characterization of highly purified SSV1 virions and identified four virus-encoded structural proteins, VP1 to VP4, as well as one DNA-binding protein of cellular origin. The virion proteins VP1, VP3, and VP4 undergo posttranslational modification by glycosylation, seemingly at multiple sites. VP1 is also proteolytically processed. In addition to the viral DNA-binding protein VP2, we show that viral particles contain the Sulfolobus solfataricus chromatin protein Sso7d. Finally, we provide evidence indicating that SSV1 virions contain glycerol dibiphytanyl glycerol tetraether (GDGT) lipids, resolving a long-standing debate on the presence of lipids within SSV1 virions. A comparison of the contents of lipids isolated from the virus and its host cell suggests that GDGTs are acquired by the virus in a selective manner from the host cytoplasmic membrane, likely during progeny egress. Viruses infecting extremophilic archaea have evolved to withstand very high temperatures, low or high pH, or near-saturating salt concentrations (1-5). Remarkably, most of these viruses do not seem to be evolutionarily related to viruses of bacteria or eukaryotes and display a considerable diversity of unique virion morphotypes (3, 4). Indeed, 11 novel viral families have been established by the International Committee for the Taxonomy of Viruses (ICTV) for the classification of archaeal viruses, emphasizing the uniqueness of rod-shaped, spindle-shaped, dropletshaped, or even bottle-shaped particles that have never been observed among viruses infecting bacteria or eukaryotes (3). Functional studies proved to be highly challenging due to the lack of similarity between the protein sequences and structures of archaeal viruses and those from other viruses and cellular organisms (6-10). Among the morphotypes that are exclusively associated with archaea, spindle-shaped viruses are particularly widespread (11) and have been isolated from highly different environments, including deep-sea hydrothermal vents (12-14), hypersaline environments (15-18), anoxic freshwaters (19), cold Antarctic lakes (20), terrestrial hot springs (21-23), and acidic mines (24).Recently, we refined the evolutionary relationships among spindle-shaped viruses by assessing the morph...

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“…Homologs of replication proteins have been identified in the genomes of various archaeal circular dsDNA viruses. These include minichromosome maintenance (MCM) proteins (TPV1), highly putative DnaA (SSV1), and Cdc6 (APBV1) homologs (16,97,98). However, the biochemical properties of these proteins are unclear.…”

Section: Genome Replicationmentioning

confidence: 99%

“…Two thermococcal viruses have been obtained so far, PAV1 and Thermococcus prieurii virus 1 (TPV1) (98,229). Interestingly, the two euryarchaeal viruses share only two ORFs that are implicated in viral infection.…”

Section: Virus-plasmid Interactionsmentioning

confidence: 99%

Archaeal Extrachromosomal Genetic Elements

Wang

Peng

Shah

et al. 2015

Microbiol Mol Biol Rev

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SUMMARY Research on archaeal extrachromosomal genetic elements (ECEs) has progressed rapidly in the past decade. To date, over 60 archaeal viruses and 60 plasmids have been isolated. These archaeal viruses exhibit an exceptional diversity in morphology, with a wide array of shapes, such as spindles, rods, filaments, spheres, head-tails, bottles, and droplets, and some of these new viruses have been classified into one order, 10 families, and 16 genera. Investigation of model archaeal viruses has yielded important insights into mechanisms underlining various steps in the viral life cycle, including infection, DNA replication and transcription, and virion egression. Many of these mechanisms are unprecedented for any known bacterial or eukaryal viruses. Studies of plasmids isolated from different archaeal hosts have also revealed a striking diversity in gene content and innovation in replication strategies. Highly divergent replication proteins are identified in both viral and plasmid genomes. Genomic studies of archaeal ECEs have revealed a modular sequence structure in which modules of DNA sequence are exchangeable within, as well as among, plasmid families and probably also between viruses and plasmids. In particular, it has been suggested that ECE-host interactions have shaped the coevolution of ECEs and their archaeal hosts. Furthermore, archaeal hosts have developed defense systems, including the innate restriction-modification (R-M) system and the adaptive CRISPR (clustered regularly interspaced short palindromic repeats) system, to restrict invasive plasmids and viruses. Together, these interactions permit a delicate balance between ECEs and their hosts, which is vitally important for maintaining an innovative gene reservoir carried by ECEs. In conclusion, while research on archaeal ECEs has just started to unravel the molecular biology of these genetic entities and their interactions with archaeal hosts, it is expected to accelerate in the next decade.

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TPV1, the first virus isolated from the hyperthermophilic genus Thermococcus

Cited by 69 publications

References 68 publications

Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea

Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea

Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids

Archaeal Extrachromosomal Genetic Elements

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