TPEF Cardiomyocyte Image 3-D Cutting and Volume Measuring Based on ImageJ

Abstract

Abstract.ImageJ is an open source image processing and analysis platform developed with Java. It can be applied to the analysis of clinical and scientific image effectively. A group of adult SD rat cardiac myocytes image using two-photon excitation fluorescence (TPEF) polarization imaging system is processed with ImageJ: 1)To achieve a panoramic display of the cardiomyocytes, we adopt 3D Viewer plugin to construct the 3D model; 2)Then, cutting the myocardial cell in any directions allows ones to observe the cell's internal structure; 3) With the following image processing, contrast enhancement, noise reduction, threshold segmentation, clear boundary of the object is presented; 4) Region of interest (ROI) of each slice is then calculated. The entire volume of the myocardial cell is accumulation of the product of area and scan step. The physical volume of the myocardial cell can be calculated with the relationship of physical unit and image pixel, such as pixels-per-inch (ppi).