TPE‐conjugated biomimetic and biodegradable polymeric micelle for AIE active cell imaging and cancer therapy

Abstract

Smart nanocarrier for simultaneous drug delivery and cellular imaging is ideal for both cancer therapy and diagnosis. In this work, polymeric micelles based on the tetraphenylethene (TPE) conjugated poly(N6-carbobenzyloxy-L-lysine)-block-poly(2-methacryloyloxyethyl phosphorylcholine) (TPE-PLys-b-PMPC) copolymer are successfully prepared. Such biomimetic and biodegradable TPE-PLys-b-PMPC micelles exhibit remarkable aggregation-induced emission (AIE) feature and great biocompatibility, showing great potential for bioimaging application. In addition, anticancer drug doxorubicin (DOX) can be incorporated into the core of micelles and the intracellular release of DOX can be furthermore traced through the fluorescent imaging of these AIE micelles. As expected, this DOX-loading polymeric micelle shows significant growth inhibition against HeLa cells and 4T1 cells and such TPE-PLys-b-PMPC micelles would be a promising drug carrier for potential cancer therapy and bioimaging. V C 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2018, 135, 45651.

Oxidation-Responsive and Aggregation-Induced Emission Polymeric Micelles with Two-Photon Excitation for Cancer Therapy and Bioimaging

“…In this work, we develop a new oxidation-responsive nanoscale polymeric micellar drug carrier based on poly­(ethylene glycol)- b -poly­( l -glutamic acid)-two-photon AIE fluorophores (mPEG- b -PLG (Se)-TP) amphiphilic copolymer with selenide group (Se) conjugated and a two-photon AIE fluorogen (TP) on the terminal group of PLG segments. The amphiphilic diblock copolymer can self-assemble into uniform and stable micelles with curcumin (Cur) − encapsulated in aqueous solution, whereas the micellar structure would be quickly destroyed by 0.1% H 2 O 2 , resulting in accelerated drug release. Moreover, the new invented two-photon fluorogen exhibited great AIE active two-photon cellular and tissue imaging with imaging depth up to 150 μm.…”

Redox-Responsive Biomimetic Polymeric Micelle for Simultaneous Anticancer Drug Delivery and Aggregation-Induced Emission Active Imaging

“…4-Nitrophenyl chloroformate-activated TPE (TPE-NO 2 ) was prepared according to previous work. 40 PMPC-b-PLAsp-SS-NH 2 (0.4 g, 0.033 mmol) and TPE-NO 2 (0.4 g, 0.781 mmol) were dissolved in a mixture of DMF (12 mL) and MeOH (10 mL) at room temperature. 200 μL of TEA (1.446 mmol) was added under the protection of argon.…”

“…The polymeric micelles were prepared by the solvent exchange method. In brief, the TPE-conjugated copolymer (10 mg) and DOX (2 mg) (dehydrochlorinated as in previous work 40 ) were dissolved in a mixture of DMF/MeOH solvent (2 mL, v/v, 1/ 1). The mixture solution was added dropwise to 5 mL of RO water under strong stirring.…”

Tunable Fluorescence-Responsive Double Hydrophilic Block Polymers Induced by the Formation of Pseudopolyrotaxanes with Cucurbit[7]Uril

“…Then, the solution was centrifuged and lyophilized. Drug-loading efficacy (DLE) was analyzed by using UV spectroscopy in accordance with the literature [64,65]. The blank micelles of the pseudopolyrotaxanes were prepared in the same way as the DOX-loaded pseudopolyrotaxanes micelles, but without the addition of the drug.…”