TP53 Polymorphism of Exon 4 at Codon 72 in Cutaneous Squamous Cell Carcinoma and Benign Epithelial Lesions of Renal Transplant Recipients and Immunocompetent Individuals: Lack of Correlation with Human Papillomavirus Status11This work was presented in part at the 19th International Papillomavirus Conference in Florianopolis, Brazil, September 1–7, 2001.

Abstract

A common polymorphism at codon 72 of exon 4 encoding either arginine or proline has been shown to confer a susceptibility to the development of skin tumor in renal transplant recipients. Moreover, this polymorphism may affect proteolytic degradation of p53 promoted by E6 protein from mucosal human papillomaviruses and represent a risk factor for human-papillomavirus-induced carcinogenesis. In this study, we analyzed the human papillomavirus presence and the TP53 allele distribution in cutaneous squamous cell carcinoma of renal transplant recipients and immunocompetent patients. Fifty-three squamous cell carcinomas from 40 renal transplant recipients, 50 benign epithelial skin lesions from 50 renal transplant recipients with no history of skin cancer, 51 squamous cell carcinomas from immunocompetent patients, and 29 blood samples from immunocompetent individuals without skin cancer were investigated. Human papillomavirus DNA was detected using polymerase chain reaction performed with two pairs of primers (MY09-MY11 and FAP59-FAP64). TP53 allele distribution was studied by denaturing gradient gel electrophoresis assay, followed by sequencing analysis. Human papillomavirus DNA was detected in 64% of squamous cell carcinoma and 79% of benign epithelial lesions from renal transplant recipients (NS) and only in 37% of squamous cell carcinoma from immunocompetent patients (p < 0.05). Mucosal oncogenic human papillomavirus types were predominant in squamous cell carcinoma from both renal transplant recipients and immunocompetent patients. Rate of arginine homozygosity in squamous cell carcinoma from renal transplant recipients was significantly higher (83%) than in immunocompetent patients with or without squamous cell carcinoma (60% and 59%, respectively). Our results suggest that TP53 arginine/arginine genotype could represent a potential risk factor for the development of squamous cell carcinoma in renal transplant recipients compared to immunocompetent patients. No association between TP53 arginine/arginine genotype and human papillomavirus status could be determined, however.

CD8+ T cell recognition of polymorphic wild-type sequence p5365–73 peptides in squamous cell carcinoma of the head and neck

“…Only 2/54 healthy donors tested encoded the P/P genotype, a finding consistent with its frequency in the population (3-8%, (24-27). Only one, however, was HLA-A2 + and suitable for this study.…”

Analysis of Tp53 Codon 72 Polymorphisms, Tp53 Mutations, and HPV Infection in Cutaneous Squamous Cell Carcinomas

“…Solid organ transplant patients also develop a large number of warts that carry a high risk of malignant transformation [18]. Studies focusing on cutaneous malignancies arising in these immunosuppressed patient populations have reported the lack of Tp53-72P to be an additional risk factor for developing squamous cell carcinoma [89], [90]. In our study population of elderly white males presumably with weakened immune systems, the lack of Tp53-72P is a risk factor for developing SCC frequently associated with HPV DNA.…”

The association between TP53 Arg72pro polymorphism and non-melanoma skin cancer risk: A meta-analysis including 7,107 subjects

“…The genotype distributions in the controls of all studies were in agreement with HWE except for three studies. [8913] The minor allele frequency of the Arg72 allele among controls ranged from 0.57 in the Asian population to 0.86 in a European population.…”